期刊
CYTOTHERAPY
卷 16, 期 7, 页码 915-926出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.jcyt.2014.02.006
关键词
adipose-derived mesenchymal stem cells; bone marrow-derived mesenchymal stem cells; mesenchymal stromal cells; Mesencult-XF; serum-free medium; clinical cell culture
类别
资金
- Karolinska Institutet Foundation
- Vinnova [2010-00501]
Background. There is a growing interest in mesenchymal stem cells (MSCs) because they are regarded as good candidates for cell therapy. Adipose tissue represents an easily accessible source to derive mesenchymal stem cells (Ad-MSCs) non-invasively in large numbers. The aim of this study was to evaluate a defined serum-free medium for in vitro expansion of MSCs as a prerequisite for their clinical use. Methods. Adipose tissue was isolated from healthy donors. Cells were isolated and expanded for five passages in serum-free medium (Mesencult-XF) and Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum (DMEM-FBS). MSC morphology, marker expression, viability, population doubling time and differentiation potential toward osteogenic and adipogenic lineages were evaluated. Bone marrow MSCs were included as controls. Results. Ad-MSCs cultured in Mesencult-XF had shorter population doubling time (33.3 +/- 13.7 h) compared with those cultured in DMEM-FBS (54.3 +/- 41.0 h, P < 0.05). Ad-MSCs cultured in Mesencult-XF displayed a stable morphology and surface marker expression and a higher differentiation potential in comparison to Ad-MSCs cultured in DMEM-FBS. Conclusions. The defined serum-free and xeno-free Mesencult-XF media appear to be a good choice for Ad-MSCs, but it is not as good in supporting culture of bone marrow MSCs when the cells are to be used for clinical purposes.
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