Review
Dermatology
J. Karim Ead, David G. Armstrong
Summary: GM-CSF is a glycoprotein with immunomodulatory properties derived from both hemopoietic and nonhemopoietic sources. In patients with diabetic foot ulcers, GM-CSF activation is impaired, leading to impaired inflammatory response. Correcting macrophage immune dysfunction with exogenous GM-CSF may help restore immune balance and promote wound healing.
INTERNATIONAL WOUND JOURNAL
(2023)
Article
Immunology
Zheng-Yan Li, Jin-Tao Wang, Gang Chen, Zhi-Guo Shan, Ting-Ting Wang, Yang Shen, Jun Chen, Zong-Bao Yan, Liu-Sheng Peng, Fang-Yuan Mao, Yong-Sheng Teng, Jin-Shan Liu, Yuan-Yuan Zhou, Yong-Liang Zhao, Yuan Zhuang
Summary: In gastric cancer tumors, neutrophils expressing CD54 and B7-H3 are closely associated with changes in GM-CSF levels, activating through the JAK-STAT3 signaling pathway. B7-H3+ neutrophils may potentially serve as biomarkers in gastric cancer, increasing with tumor progression and predicting overall survival.
CLINICAL IMMUNOLOGY
(2021)
Article
Virology
Sarah Stonedahl, J. Smith Leser, Penny Clarke, Huntington Potter, Timothy D. D. Boyd, Kenneth L. L. Tyler
Summary: West Nile virus (WNV) is the main cause of epidemic arboviral encephalitis in the United States. Depleting microglia in WNV-infected mice enhances viral replication and tissue injury, while augmenting microglial activation through GM-CSF administration reduces viral titers and apoptotic activity and increases survival. Stimulation of microglial activation may be a viable therapeutic approach for WNV neuroinvasive disease.
JOURNAL OF VIROLOGY
(2023)
Article
Allergy
Sharon Van Nevel, Jozefien Declercq, Gabriele Holtappels, Bart N. Lambrecht, Claus Bachert
Summary: This study aimed to identify underlying molecular pathways of type 2-low chronic rhinosinusitis (CRS) and improve personalized treatments. The results showed that G-CSF is an important cytokine regulating neutrophils in type 2-low CRS and has potential in the diagnosis and therapy of the disease.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2022)
Article
Immunology
An-ran Xi, Yi-jun Luo, Jin-Tao Guan, Wei-Jie Wang, Zheng-Hao Xu
Summary: This study analyzed the efficacy and safety of granulocyte-macrophage colony-stimulating factor (GM-CSF) antibodies in COVID-19 patients. The results showed that GM-CSF antibody treatment can reduce the incidence of invasive mechanical ventilation and have slight but not significant effects on mortality and ventilation improvement. Sensitivity analysis revealed statistically significant results for mortality and ventilation improvement rate when one study was excluded. Furthermore, GM-CSF antibodies did not increase the risks of secondary infection and adverse events.
INFLAMMOPHARMACOLOGY
(2023)
Article
Medicine, General & Internal
Slawomir Lewicki, Monika Lesniak, Agnieszka Sobolewska-Ruta, Aneta Lewicka, Marta Grodzik, Eugeniusz K. Machaj, Marek Saracyn, Jacek Z. Kubiak, Zygmunt Pojda
Summary: Encapsulation of cytokines in liposomes is an efficient method to deliver cytokines to cells at working concentrations without affecting cell morphology or proliferation. This method has great potential and important applications in tissue regeneration and other fields.
ARCHIVES OF MEDICAL SCIENCE
(2022)
Article
Pharmacology & Pharmacy
Jin-Tao Guan, Wei-Jie Wang, Du Jin, Xiao-Yue Mou, Shan-Shan Lei, Zheng-Hao Xu
Summary: This meta-analysis showed that GM-CSF antibody treatment in COVID-19 patients could reduce the risk of death and increase ventilation, without significantly increasing the risk of secondary infection or ICU admission. Older age and length of stay may have an impact on these outcomes.
THERAPEUTIC ADVANCES IN CHRONIC DISEASE
(2021)
Article
Immunology
Peilin Chen, Chunyu Huang, Qing Sun, Huixian Zhong, Feng Xiong, Su Liu, Zhihong Yao, Zhiqiang Liu, Caiyun Wan, Yong Zeng, Lianghui Diao
Summary: This study investigated the concentration of GM-CSF in human single-blastocyst conditioned medium (SBCM) and its association with embryo quality and pregnancy outcome. GM-CSF was found to be a potential biomarker for predicting pregnancy outcome and selecting embryos with high developmental potential for transfer in assisted reproductive technology (ART).
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Bing Chen, Yong Yang, Congwen Yang, Jiaxiang Duan, Lin Chen, Kaizhi Lu, Bin Yi, Yang Chen, Duo Xu, He Huang
Summary: Hepatopulmonary syndrome (HPS) significantly increases mortality of patients, yet its pathogenesis is not fully understood. This study compared CBDL and TAA rat models to investigate the mechanisms of HPS. Neutrophil recruitment in the early stage of rat HPS leads to pulmonary vascular dilatation and hypoxemia, while accumulation of M2 macrophages induced by GM-CSF/GM-CSFR and MCP1/CCR2 promotes pulmonary fibrosis and vascular dilatation, ultimately resulting in HPS development.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Cell & Tissue Engineering
Sara Bucar, Andre Dargen de Matos Branco, Marcia F. Mata, Joao Coutinho Milhano, Iris Caramalho, Joaquim M. S. Cabral, Ana Fernandes-Platzgummer, Claudia L. da Silva
Summary: The study compared the capacity of MSC from different sources to expand UCB CD34(+) cells ex vivo, with AT-derived MSC showing superior expansion potential and maintenance of primitive cells. UCM-derived MSC demonstrated inferior hematopoietic supportive capacity compared to MSC from adult tissues. Further research is needed to determine the impact of HPL on the hematopoietic supportive capacity of MSC.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Yiqi Yang, Bihui Zhang, Junye Xie, Jingsheng Li, Jia Liu, Rongzhan Liu, Linhao Zhang, Jinting Zhang, Zijian Su, Fu Li, Leisheng Zhang, An Hong, Xiaojia Chen
Summary: The CH02 peptide is effective in ex vivo expansion of CD34(+) umbilical cord blood hematopoietic stem/progenitor cells and promotes wound healing in diabetic mice through bidirectional orchestration of proinflammatory and anti-inflammatory factors.
ACTA BIOCHIMICA ET BIOPHYSICA SINICA
(2023)
Review
Biochemistry & Molecular Biology
Gustavo de Souza Vieira, Talita de Carvalho Kimura, Joao Figueira Scarini, Reydson Alcides de Lima-Souza, Luccas Lavareze, Carolina Emerick, Mayara Trevizol Goncalves, Ingrid Iara Damas, Tayna Figueiredo-Maciel, Raisa Sales de Sa, Iara Goncalves Aquino, Joao Paulo Goncalves de Paiva, Patricia Maria Fernandes, Moises Willian Aparecido Goncalves, Luiz Paulo Kowalski, Albina Altemani, Gary Chris Fillmore, Fernanda Viviane Mariano, Erika Said Abu Egal
Summary: This article provides an in-depth review of the role of hematopoietic cytokines in the tumor microenvironment of head and neck cancers, and summarizes the current clinical trials and findings on the therapeutic application of these cytokines.
Article
Biotechnology & Applied Microbiology
Rui Chen, Yujie Li, Yangyang Zhuang, Yiming Zhang, Hailong Wu, Tao Lin, Shixuan Chen
Summary: This study combines GM-CSF and 3D nanofiber scaffolds to promote tissue regeneration. Through subcutaneous implantation in humanized mice, it was found that GM-CSF could accelerate cell migration and cytokine release, and ultimately enhance tissue regeneration. Therefore, GM-CSF loaded 3D nanofiber scaffolds have potential applications in tissue regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Oncology
Lan Huang, Guisi Chen, Ying Chen, Wanwen Wu, Changli Tao, Hongwei Shao, Shulin Huang, Han Shen
Summary: Tumor immunotherapy targeting tumor-associated antigens and the impact of GM-CSF and CCL17 on tumor immunity have been widely studied. Combining survivin-specific CTL with CCL17 and GM-CSF genes can inhibit tumor growth more effectively through stable cell lines expressing these genes.
Article
Dermatology
K. Wolk, T. -C. Brembach, D. Simaite, E. Bartnik, S. Cucinotta, A. Pokrywka, M. L. Irmer, J. Triebus, E. Witte-Haendel, G. Salinas, T. Leeuw, H. -D. Volk, K. Ghoreschi, R. Sabat
Summary: The expression of G-CSF is high in HS skin, especially in inflamed nodules and abscesses. IL-1 and IL-17 induce G-CSF production, and G-CSF blood levels correlate with the severity of HS.
BRITISH JOURNAL OF DERMATOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ivan Martinez-Monge, Carlos Martinez, Marianne Decker, Isuru A. Udugama, Igor Marin de Mas, Krist Gernaey, Lars K. Nielsen
Summary: A new soft-sensor-based fed-batch strategy has been developed to maintain substrate concentration at optimal levels in cell cultures.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Michael A. MacDonald, Craig Barry, Teddy Groves, Veronica S. Martinez, Peter P. Gray, Kym Baker, Evan Shave, Stephen Mahler, Trent Munro, Esteban Marcellin, Lars K. Nielsen
Summary: This study used CRISPR-Cas9 technology to create gene knockouts in CHO cells and observed the cells' response to apoptotic stimuli. The results showed that Bak1 and Bax have a synergistic antiapoptotic effect, while the deletion of Bok had no significant impact on cell death. Importantly, the study demonstrated the importance of a delay function in accurately modeling the cell death response.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Review
Biotechnology & Applied Microbiology
Michael A. MacDonald, Matthias Nobel, Dinora Roche Recinos, Veronica S. Martinez, Benjamin L. Schulz, Christopher B. Howard, Kym Baker, Evan Shave, Yih Yean Lee, Esteban Marcellin, Stephen Mahler, Lars Keld Nielsen, Trent Munro
Summary: Over the past 30 years, the biopharmaceutical industry's success has been largely dependent on products derived from Chinese Hamster Ovary (CHO) cell lines, with perfusion processes playing a key role in improving mammalian cell cultures for large-scale fed-batch processes. Recent interest has been regained in perfusion-based bioprocess research, driven by high volumetric titers and a small footprint, especially during the recent pandemic. The future of perfusion bioprocesses looks promising, with emerging trends in media optimization, continuous processing, and cell line engineering holding the greatest potential.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Craig P. Barry, Rosemary Gillane, Gert H. Talbo, Manual Plan, Robin Palfreyman, Andrea K. Haber-Stuk, John Power, Lars K. Nielsen, Esteban Marcellin
Summary: The study demonstrates that utilizing a multi-omics approach in combination with antiSMASH allows for the assessment of the secondary metabolic potential of a Streptomyces strain producing mannopeptimycin, an important cyclic peptide effective against Gram-positive infections. By combining genomics, proteomics, and metabolomics, it is possible to rapidly evaluate a strain's performance in bioreactors for industrial production of secondary metabolites.
Review
Biochemical Research Methods
Esteban Marcellin, Largus T. Angenent, Lars K. Nielsen, Bastian Molitor
Summary: This review explores the potential of gas fermentation and synthetic biology to transform food production and achieve sustainability.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Medicine, Research & Experimental
Michael A. MacDonald, Matthias Nobel, Veronica S. Martinez, Kym Baker, Evan Shave, Peter P. Gray, Stephen Mahler, Trent Munro, Lars K. Nielsen, Esteban Marcellin
Summary: The development of death-resistant cell lines using CRISPR technology has proven to be beneficial for monoclonal antibody production. These cell lines showed advantages in fed-batch and perfusion bioreactors, with higher cell densities or longer culture durations in fed-batch, and the ability to culture without bleed in perfusion. This study highlights the potential of rationally engineered cell lines for improving mAb manufacturing processes.
Article
Biochemical Research Methods
Veronica S. Martinez, Pedro A. Saa, Jason Jooste, Kanupriya Tiwari, Lake-Ee Quek, Lars K. Nielsen
Summary: The topology of metabolic networks is modular, with weak connections between modules. This study defines metabolic modules using network topology and validates them using genome-scale metabolic models. The study also explores the role of reversible reactions in metabolic flexibility.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Microbiology
Lorena Azevedo de Lima, Kristina Reinmets, Lars Keld Nielsen, Esteban Marcellin, Audrey Harris, Michael Kopke, Kaspar Valgepea
Summary: RNA sequencing (RNA-seq) is a widely used technique for transcriptome analysis, but the choice of library preparation kits can significantly impact the results. Different commercial products show significant differences in rRNA removal, strand specificity, and transcript abundance distribution. Selecting appropriate sample processing workflows and products is crucial for RNA-seq studies.
MICROBIOLOGY SPECTRUM
(2022)
Article
Engineering, Chemical
Sarang S. Nath, Lars K. Nielsen, John Villadsen
Summary: A novel methodology is developed to convert chemical reactions into electrical circuits, allowing for the study of complex cyclic bionetworks. The approach is demonstrated using a pyramidal reaction network catalyzed by an important enzyme in cancer therapy. The analysis identifies the key mechanisms contributing to the overall reaction rate.
CHEMICAL ENGINEERING SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Tae Kwang Ha, Andreu Odena, Karen Julie la Cour Karottki, Che Lin Kim, Hooman Hefzi, Gyun Min Lee, Helene Faustrup Kildegaard, Lars K. Nielsen, Lise Marie Grav, Nathan E. Lewis
Summary: The use of a dual selection method can greatly improve the efficiency of Chinese hamster ovary (CHO) cell lines in producing biotherapeutic proteins, although it may lead to reduced growth rate and viability. By knocking out the Gs/Aspg genes, a novel dual selectable system was successfully constructed, which showed significantly improved productivity and titer compared to the standard Gs system under the same selection conditions. With further optimization of culture medium and selection conditions, this approach is expected to be an effective addition to industrial workflows for producing recombinant biotherapeutics.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Hanne H. Henriksen, Igor Marin de Mas, Lars K. Nielsen, Joseph Krocker, Jakob Stensballe, Sigurour T. Karvelsson, Niels H. Secher, Ottar Rolfsson, Charles E. Wade, Par I. Johansson
Summary: In trauma patients, shock-induced endotheliopathy (SHINE) is associated with poor prognosis. This study identified four metabolic phenotypes in trauma patients and analyzed their intracellular metabolic profiles using a computational network analysis. One of the phenotypes, characterized by an inability to maintain redox balance, was associated with increased mortality. This research highlights the importance of understanding metabolic patterns in trauma patients for prognosis and potential therapeutic interventions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Carmen Bedia, Nuria Dalmau, Lars K. Nielsen, Roma Tauler, Igor Marin de Mas
Summary: Although the dose-effect relationship between Endocrine disruptors (EDs) and tumor malignancy is supported by numerous studies, the impact of chronic exposure to non-lethal concentrations of EDs in cancer remains unknown. This study investigates the molecular processes underlying malignancy in prostate cancer using two complementary computational approaches. Experimental validation confirms predictions, revealing a disruption in metabolic and regulatory pathways.
Article
Biotechnology & Applied Microbiology
Igor Marin de Mas, Helena Herand, Jorge Carrasco, Lars K. Nielsen, Par I. Johansson
Summary: Genome-scale metabolic models (GEMs) are valuable tools for understanding human metabolism and have applications in studying diseases and designing metabolic engineering. This paper presents a novel algorithm-aided protocol that allows for the continuous updating of highly curated GEMs, overcoming the limitations of existing methods.
BIOENGINEERING-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Marina de Leeuw, Marta R. A. Matos, Lars Keld Nielsen
Summary: Kinetic models are important for understanding metabolic systems. This paper presents a detailed kinetic model for the central carbon metabolism of Escherichia coli, which has been validated and can be used for studying cellular metabolism and metabolic pathway design.
METABOLIC ENGINEERING
(2023)
Article
Engineering, Environmental
James K. Heffernan, Chun-Yu Lai, R. Axayacatl Gonzalez-Garcia, Lars Keld Nielsen, Jianhua Guo, Esteban Marcellin
Summary: Biogas production from anaerobic digestion is important for energy generation, nutrient recovery, and waste resource valorisation. Removing or altering carbon dioxide content facilitates biogas use as biomethane and can contribute to transitioning into a low carbon and circular economy. Gas fermentation utilising acetogens and renewable H2 offers an alternative for biogas upgrading, enriching the biogas and transforming CO2 into valuable fuels and chemicals in a one-step process.
CHEMICAL ENGINEERING JOURNAL
(2023)