Article
Biotechnology & Applied Microbiology
Yangyang Liu, Qihao Sun, Mengyang Hao, Wen-Song Tan, Haibo Cai
Summary: A novel magnetically controlled bioreactor was developed to support the ex vivo expansion of NK cells. The results showed that the expansion of NK-92 cells in the bioreactor was significantly higher than that in traditional T25 culture flasks, and the function and cell killing activity of expanded cells were not significantly different, indicating the potential of the magnetically controlled bioreactor as an effective strategy for NK cell production.
BIORESOURCES AND BIOPROCESSING
(2022)
Article
Biotechnology & Applied Microbiology
Yan Fu, Yuying Chen, Zhepei Xie, Huimin Huang, Wen-Song Tan, Haibo Cai
Summary: NK-92 cell expansion for immunotherapy is crucial, and this study optimized a vitamin combination to promote the ex vivo expansion of NK-92 cells. The study found that NK-92 cells underwent metabolic reprogramming in the optimized medium, shifting towards the PPP and TCA cycle, and relying more on mitochondrial respiration. This metabolic shift provided more metabolites and energy, accelerating cell expansion. The results of this study provide valuable insights for large-scale ex vivo expansion of clinically available NK-92 cells.
BIORESOURCES AND BIOPROCESSING
(2022)
Review
Chemistry, Multidisciplinary
Bohwa Han, Yeonju Song, Jeehun Park, Junsang Doh
Summary: This review discusses the use of nanomaterials to address the limitations of CAR-T cell therapy, including methods to improve expansion, gene delivery, and enhance antitumor functions. Understanding the biological principles behind various nanomaterials is crucial for their application in ex vivo engineering of T/NK cells.
JOURNAL OF CONTROLLED RELEASE
(2022)
Article
Oncology
Yuan Wang, Ryohichi Sugimura
Summary: Hematopoietic stem cells (HSCs) are promising for treating various hereditary and hematologic diseases by differentiating into mature blood cells. However, the low population of HSCs in cord blood and bone marrow has led to investigations on ex vivo HSC expansion. This review surveys the current techniques for HSC self-renewal and summarizes the effects of intrinsic and extrinsic factors on HSC expansion, as well as the challenges and opportunities in this field.
EXPERIMENTAL CELL RESEARCH
(2023)
Review
Chemistry, Multidisciplinary
Jingyi Wang, Ting Lei
Summary: This article discusses the separation methods for enriching large-diameter s-SWCNTs and their advantages in electronic applications, including CPW, GC, ATPE, and DGU technologies. By separating based on the electronic type and chirality of SWCNTs, important references can be provided for the future development of SWCNT-based electronic devices.
Review
Pharmacology & Pharmacy
Jiaxing Li, Xiao Wang, Jiayu Ding, Yasheng Zhu, Wenjian Min, Wenbing Kuang, Kai Yuan, Chengliang Sun, Peng Yang
Summary: Hematopoietic stem cell transplantation is the only curative therapy for many diseases. Umbilical cord blood (UCB) source of HSCs has advantages over bone marrow, but has limited dose. Small molecule-mediated HSC expansion has shown promise in overcoming this limitation.
ACTA PHARMACEUTICA SINICA B
(2022)
Article
Biochemistry & Molecular Biology
Yoichi Shida, Tsutomu Nakazawa, Ryosuke Matsuda, Takayuki Morimoto, Fumihiko Nishimura, Mitsutoshi Nakamura, Ryosuke Maeoka, Shuichi Yamada, Ichiro Nakagawa, Young-Soo Park, Motoaki Yasukawa, Takashi Tojo, Takahiro Tsujimura, Hiroyuki Nakase
Summary: GiNKs, as a promising cell-based immunotherapy, show potential in treating GBM by prolonging the survival of NOG mice carrying U87MG-derived tumors. Although PD-L1 is expressed on U87MG and T98G cells, the expression levels vary significantly. PD-1 blocking antibodies did not have an additional effect on prolonging survival.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Biomedical
Hye Sung Kim, Tzu-Chieh Ho, Moshe J. Willner, Michael W. Becker, Hae-Won Kim, Kam W. Leong
Summary: In this study, we propose an ex vivo T cell expansion system that mimics natural antigen-presenting cells for adoptive cell therapy. The system utilizes microfiber scaffolds coated with dendritic cell membrane to replicate the physicochemical properties of dendritic cells, leading to greater expansion and functionality of T cells. This scalable and customizable platform holds promise for future research and application in cell therapy.
BIOACTIVE MATERIALS
(2023)
Article
Oncology
Renata Scopim-Ribeiro, Michael M. Lizardo, Hai-Feng Zhang, Anne-Chloe Dhez, Chistopher S. Hughes, Poul H. Sorensen
Summary: Ewing sarcoma is a highly malignant bone and soft tissue tumor with poor 5-year overall survival rates. Successful implementation of the PuMA model for EwS cells using NOD-SCID-IL2 receptor gamma null mice showed high engraftment rates, suggesting potential immune permissiveness needed by EwS cells.
FRONTIERS IN ONCOLOGY
(2021)
Article
Immunology
Kerstin H. Mair, Assiatu J. Crossman, Bettina Wagner, Susanna Babasyan, Leela Noronha, Patricia Boyd, Dante Zarlenga, Maria Stadler, Katinka A. van Dongen, Wilhelm Gerner, Armin Saalmueller, Joan K. Lunney
Summary: NK cells play a vital role in the innate immune system of vertebrates, rapidly eliminating tumors or virus infected cells. The NKp30, NKp44, and NKp46 receptors are important mediators of NK cell cytotoxicity. In this study, porcine-specific anti-NKp44 antibodies were generated and tested on different age groups of pigs. The results showed that NKp44 expression was detected on resting NK cells and increased upon in vitro stimulation. This study provides valuable insights into the differentiation, functionality, and activation of porcine NK cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Lena Rauch-Wirth, Alexander Renner, Kubra Kaygisiz, Tatjana Weil, Laura Zimmermann, Armando A. Rodriguez-Alfonso, Desiree Schuetz, Sebastian Wiese, Ludger Staendker, Tanja Weil, Dominik Schmiedel, Jan Muench
Summary: Peptide nanofibrils (PNFs) are transduction enhancers that can improve the transduction rate of chimeric antigen receptor (CAR)-T cell therapy for cancer. By binding to negatively charged virions, PNFs facilitate virion attachment to cells, leading to increased gene transfer rates. In this study, a short peptide called RM-8 was identified using in silico screening, which effectively boosted T cell transduction rates. The subsequent refinement, D4 peptide, showed stable assembly and superior transduction rates in primary T and NK cells.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Andrew B. Burns, Corinna Doris, Kevin Vehar, Vinit Saxena, Cameron Bardliving, Parviz A. Shamlou, M. Ian Phillips
Summary: The study demonstrates that a novel bioreactor design can successfully cultivate bone marrow-derived hMSCs, with high cell yield, purity, and differentiation potential.
Article
Chemistry, Multidisciplinary
Ozgun Kocabiyik, Valeria Cagno, Paulo Jacob Silva, Yong Zhu, Laura Sedano, Yoshita Bhide, Joelle Mettier, Chiara Medaglia, Bruno Da Costa, Samuel Constant, Song Huang, Laurent Kaiser, Wouter L. J. Hinrichs, Anke Huckeriede, Ronan Le Goffic, Caroline Tapparel, Francesco Stellacci
Summary: This study describes a new class of broad-spectrum anti-influenza macromolecules that meet the criteria of targeting conserved viral domains and being virucidal with low toxicity. These compounds based on cyclodextrin core show effective inhibition of viruses and irreversibility of inhibition. In vitro efficacy is demonstrated against various human or avian influenza strains, as well as a 2009 pandemic strain ex vivo. Furthermore, therapeutic efficacy is shown in mice with one compound providing 90% survival when administered post-infection.
Article
Cell & Tissue Engineering
Kajal Chaudhry, Ehsan Dowlati, Mark D. Long, Ashley Geiger, Haili Lang, Eduardo C. Gomez, Nethaji Muniraj, Carlos E. Sanchez, Prashant K. Singh, Song Liu, Catherine M. Bollard, Conrad Russell Y. Cruz
Summary: Researchers found that ex vivo expansion can overcome the immune suppression effects of exogenous TGF-beta on PB- and CB-derived NK cell therapy products. They also discovered that exposure to exogenous TGF-beta leads to upregulation of inhibitory receptors on NK cells, thus creating a novel mechanism of immune suppression.
Article
Immunology
Paul D. Bates, Alexander L. Rakhmilevich, Monica M. Cho, Myriam N. Bouchlaka, Seema L. Rao, Joanna M. Hales, Rimas J. Orentas, Terry J. Fry, Stephen D. Gilles, Paul M. Sondel, Christian M. Capitini
Summary: Using allogeneic hematopoietic stem cell transplant (HSCT) for high-risk neuroblastoma (NBL) can increase survival rates, and combining specific immunocytokines and ex vivo activated NK cells can control tumor growth, decrease peripheral effector cell subsets, but must be balanced with the risk of inducing cytokine release syndrome (CRS).
FRONTIERS IN IMMUNOLOGY
(2021)
