期刊
CYTOTECHNOLOGY
卷 63, 期 4, 页码 415-423出版社
SPRINGER
DOI: 10.1007/s10616-011-9362-9
关键词
Pig; IPEC-J2; Boyden chamber; Model system
资金
- Fund for Scientific Research Flanders (Fonds Wetenschappelijk Onderzoek-Vlaanderen-Belgium) [G026307N]
The high similarity between pigs and humans makes pigs a good gastrointestinal (GI) model for humans. Recently an epithelial cell line originating from the jejunum of pig (IPEC-J2) became available. Once validated, this model can be used to investigate the complex interactions occurring in the intestine. The advantages of using IPEC-J2 as in vitro model of the GI tract are the high resemblance between humans and pigs, and the ease of extrapolating in vitro to in vivo characteristics. In this study, the IPEC-J2 cells were functionally characterized by measuring the trans-epithelial electrical resistance (TEER), and by histological and ultrastructural studies. IPEC-J2 cells grown on six different permeable support systems, were investigated. The Transwell(A (R))-COL collagen-coated membrane (1.12 cm(2)) showed the best results concerning time efficiency and TEER values. The optimum seeding density of 12 x 10(5) cells/mL ensured that after 9 days of differentiation a confluent monolayer was formed. The decrease in TEER values after a maximum had been reached, coincided with the ultrastructural development of apical microvilli. We conclude that IPEC-J2 cells grown on collagen-coated membranes represent a valuable in vitro model system for the small intestinal epithelium which can be of great interest for intestinal research.
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