Article
Multidisciplinary Sciences
Bojian Ding, Heidy Y. Narvaez-Ortiz, Yuvraj Singh, Glen M. Hocky, Saikat Chowdhury, Brad J. Nolen
Summary: Arp2/3 complex nucleates branched actin filaments and provides pushing forces for cellular processes. This study reveals the contacts between Arp2/3 complex and the mother actin filament, suggesting that actin filaments stimulate subunit flattening for complex activation. However, limited contact between the bottom half of the complex and the mother filament may explain why actin filaments are required but insufficient to trigger nucleation during WASP-mediated activation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Steven Z. Chou, Moon Chatterjee, Thomas D. Pollard
Summary: By analyzing the images collected by electron cryo-microscopy, we reconstructed the structure of actin filament branch junctions formed by fission yeast Arp2/3 complex. The formation of this structure requires a conformational change of the Arp2/3 complex and the acquisition of a new buried surface area to maintain the stability of the branch.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Biochemistry & Molecular Biology
Scott B. Thompson, Monique M. Waldman, Jordan Jacobelli
Summary: T cells utilize actin network remodeling to generate shape changes and mechanical forces for migration through diverse tissue environments. By studying actin polymerization effectors, we gain a deeper understanding of the mechanisms underlying T-cell migration.
Article
Biology
Rikki M. Garner, Julie A. Theriot
Summary: Animal cell migration is driven by the polymerization of actin filaments, but the noisy nature of this process poses challenges for understanding cellular behavior. By studying the shape fluctuations of HL-60 cells, we discovered a novel noise-suppression mechanism based on system geometry and explained the conservation of lamellipodial shape.
Article
Biochemistry & Molecular Biology
Shashi Prakash Singh, Peggy Paschke, Luke Tweedy, Robert H. Insall
Summary: PKB and SGK kinases play crucial roles in pseudopodia formation and cell motility, particularly in actin polymerization and recruitment of the Arp2/3 complex by the Scar/WAVE complex.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Cell Biology
Vanessa Dimchev, Ines Lahmann, Stefan A. Koestler, Frieda Kage, Georgi Dimchev, Anika Steffen, Theresia E. B. Stradal, Franz Vauti, Hans-Henning Arnold, Klemens Rottner
Summary: This study investigated the effects of acute Arp2/3 complex removal on the actin cytoskeleton regulation, showing both direct and indirect impacts on cell morphology, migration directionality, and chemotaxis. Depletion of Arp2/3 complex resulted in altered cell behavior and cytoskeletal dynamics, highlighting the complexities of actin network maintenance and cellular motility.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Emmanuel Martin, Magali Suzanne
Summary: This review discusses the role of the actin nucleation complex Arp2/3 in epithelial tissue, including its influence on cell-cell cooperation and tissue morphogenesis. It plays a significant role in regulating cell-cell adhesion, intracellular trafficking, and protrusion formation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Cell Biology
Thomas C. A. Blake, Jennifer L. Gallop
Summary: Filopodia are dynamic cell surface protrusions used for cell motility, pathogen infection, and tissue development. The molecular mechanisms determining filopodia growth and retraction are complex and involve integration of mechanical forces, membrane curvature, extracellular signaling, and the state of the cytoskeleton. Advances in technology have improved our understanding of filopodia through in vitro and in vivo studies.
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Meng-Hao Pan, Rui Xu, Yiqian Zhang, Lu Yin, Ruoyu Li, Dongxu Wen, Sihai Lu, Yan Gao, Xiaoe Zhao, Qiang Wei, Bin Han, Baohua Ma
Summary: Our study demonstrates that the Arp2/3 complex is essential for maintaining cytoskeletal dynamics and mitochondrial function during goat oocyte maturation. Disruption of the Arp2/3 complex leads to impaired F-actin assembly, spindle organization, ROS accumulation, and increased early apoptosis in goat oocytes.
Article
Cell Biology
Carsten Schwan, Alexander E. Lang, Andreas Schlosser, Setsuko Fujita-Becker, Abdulatif AlHaj, Rasmus R. Schroeder, Jan Faix, Klaus Aktories, Hans Georg Mannherz
Summary: Researchers found that binary toxins produced by Clostridioides bacteria can ADP-ribosylate the Arp2/3 complex and its associated proteins, thereby inhibiting their activity. This discovery reveals the mechanism by which Clostridioides affects the cellular cytoskeleton.
Article
Genetics & Heredity
Virginia L. King, Nathan K. Leclair, Alyssa M. Coulter, Kenneth G. Campellone
Summary: The actin cytoskeleton plays an important role in vital cellular processes, with the study uncovering the involvement of WASP family members JMY and WHAMM in DNA damage-induced apoptosis. These proteins contribute to a p53-dependent cell death pathway by promoting mitochondrial permeability, caspase cleavage, and actin nucleation. Additionally, the study reveals changes in gene expression upon JMY loss, including upregulation of the G-protein RhoD which impacts cell survival.
Article
Biochemistry & Molecular Biology
Jungsik Noh, Tadamoto Isogai, Joseph Chi, Kushal Bhatt, Gaudenz Danuser
Summary: The regulatory network governing lamellipodial and lamellar actin structures is complex, with multiple actin regulators and overlapping functions. By applying Granger causal inference to image fluctuations, we reconstructed the cause-effect relations among actin regulators and revealed their roles in controlling edge motion.
Review
Oncology
Dejana Jovanovic, Shen Yan, Martin Baumgartner
Summary: The finely tuned integration of intra- and extracellular cues by components of the MAPK signaling pathways controls uncontrolled growth and tumor cell dissemination. MAP4K4 plays a role as an upstream integrator of extracellular cues involved in proliferation and cell motility control. Its involvement in regulating cytoskeleton dynamics and cell motility is particularly relevant in understanding its role in cancer metastasis. However, it also acts as an activator of the Hippo tumor suppressor pathway, complicating its classification as an oncogenic driver kinase. Understanding the diverse functions of MAP4K4 and their implications in oncogenesis and tumor progression is important for developing potential therapeutic strategies.
FRONTIERS IN ONCOLOGY
(2022)
Article
Cell Biology
Shuting Zhang, Dimitrios Vavylonis
Summary: The nucleation of actin filament branches by the Arp2/3 complex involves activation through nucleation promotion factors (NPFs), recruitment of actin monomers, and binding of the complex to the side of actin filaments. Our molecular dynamics simulations using a coarse-grained model revealed stable configurations of the Arp2/3 complex with actin monomers and the activating VCA domain of the NPF Wiskott-Aldrich syndrome protein, supporting prior structural studies and providing insight into mechanisms proposed in previous studies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Pengxiang Zhao, Hongbo Han, Xiang Wu, Jian Wu, Zhuqing Ren
Summary: This study identified the molecular mechanisms underlying LD motility in cancer cells and revealed the impact of ARP2/3 on the prognosis of patients with cervical squamous cell carcinoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Casey A. Ydenberg, Shae B. Padrick, Meredith O. Sweeney, Meghal Gandhi, Olga Sokolova, Bruce L. Goode
Article
Cell Biology
Joanna Mathis Paterson, Casey A. Ydenberg, Mark D. Rose
JOURNAL OF CELL BIOLOGY
(2008)
Article
Cell Biology
Casey A. Ydenberg, Mark D. Rose
JOURNAL OF CELL BIOLOGY
(2009)
Article
Cell Biology
Casey A. Ydenberg, Richard A. Stein, Mark D. Rose
MOLECULAR BIOLOGY OF THE CELL
(2012)
