Article
Multidisciplinary Sciences
Matteo Bernardello, Emilio J. Gualda, Pablo Loza-Alvarez
Summary: Light-sheet fluorescence microscopy is an important tool used in biological and biomedical research. However, the sample mounting procedure can be cumbersome and time-consuming. This article presents a new imaging paradigm for LSFM that offers multimodal imaging and a straightforward sample mounting strategy, enhancing the flexibility and throughput of the system.
SCIENTIFIC REPORTS
(2022)
Review
Nanoscience & Nanotechnology
Shizheng Zhou, Bingbing Chen, Edgar S. Fu, Hong Yan
Summary: This paper reviews the integration of microfluidic chips and computer vision in life sciences and biology research, specifically in the analysis of cell imaging data. Microfluidic chips generate large amounts of visual data at the single-cell level, while computer vision techniques rapidly process and analyze these data to extract valuable information about cellular health and function. This integrative approach allows for noninvasive and low-damage cellular characterization and provides a highly controlled environment for cell growth and manipulation, enhancing data analysis accuracy. It is expected that the combination of microelectromechanical devices, microfluidic chips, and computer vision will enable label-free, automatic, low-cost, and fast cellular information recognition and high-throughput analysis of cellular responses to different compounds for various applications in drug discovery, diagnostics, and personalized medicine.
MICROSYSTEMS & NANOENGINEERING
(2023)
Article
Medicine, General & Internal
Julien Demagny, Camille Roussel, Mailys Le Guyader, Eric Guiheneuf, Veronique Harrivel, Thomas Boyer, Momar Diouf, Michael Dussiot, Yohann Demont, Loic Garon
Summary: This study presents a method combining imaging flow cytometry and artificial intelligence for the direct label-free and operator-independent quantification of schistocytes in whole blood. By training a support vector machine blood elements' classifier, the method can reliably diagnose thrombotic microangiopathy syndrome in emergency situations.
Article
Biology
Ziqi Zhang, Kelvin C. M. Lee, Dickson M. D. Siu, Michelle C. K. Lo, Queenie T. K. Lai, Edmund Y. Lam, Kevin K. Tsia
Summary: This article introduces an image-based approach that can quantify multiple biophysical fractal-related properties of single cells at subcellular resolution. This technique has high-throughput single-cell imaging performance and offers sufficient statistical power for cell classification, drug response assays, and cell-cycle progression tracking.
COMMUNICATIONS BIOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Masashi Ugawa, Sadao Ota
Summary: This study introduces a high-throughput 3D-imaging flow cytometry technique based on light-sheet microscopy, which can rapidly screen a large number of cells and obtain subcellular structural information.
Article
Multidisciplinary Sciences
Yannik Severin, Benjamin D. Hale, Julien Mena, David Goslings, Beat M. Frey, Berend Snijder
Summary: Phenotypic plasticity in the immune system is influenced by donor age, gender, and blood pressure, resulting in distinct polarization and activation-associated phenotypes across immune cell classes. The morphology of T cells is associated with their transcriptional state, with inflammation-associated polarized T cell morphology and age-associated loss of mitochondria observed. This study reveals that immune cell phenotypes reflect both molecular and personal health information, providing new perspectives for deep immune phenotyping in individuals' health and disease.
Review
Chemistry, Analytical
Minruihong Wang, Hongyan Liang, Xiao Chen, Deyong Chen, Junbo Wang, Yuan Zhang, Jian Chen
Summary: Single-cell analysis plays a crucial role in scientific research and clinical diagnosis, especially in the study of dynamic immune processes and tumor research. The development of optoelectronic flow cytometry technology offers new possibilities for complete blood cell counting and is expected to become the standard for future blood analysis.
Article
Biochemical Research Methods
Madeline E. McCarthy, William B. Dodd, Xiaoming Lu, Daniel J. Pritko, Nishi D. Patel, Charlotte V. Haskell, Hugo Sanabria, Mark A. Blenner, Marc R. Birtwistle
Summary: We propose a theory for high-throughput genetic interaction screens, which extends the Multiplexing using Spectral Imaging and Combinatorics (MuSIC) approach to create 100,000 spectrally unique MuSIC barcodes. Experimental testing of this theory could revolutionize genetic perturbation technology and knowledge of genetic function. Additionally, a genome-scale spectral barcode library could have widespread applications in genetic screening and lineage tracing.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Joshua A. Welsh, Bryce Killingsworth, Julia Kepley, Tim Traynor, Kathy McKinnon, Jason Savage, Deven Appel, Kenneth Aldape, Kevin Camphausen, Jay A. Berzofsky, Alexander R. Ivanov, Ionita H. Ghiran, Jennifer C. Jones
Summary: Evidence is increasing for the clinical utility of extracellular vesicles (EVs) as translational biomarkers, but there is a lack of methods for removing unbound labels. By developing a multi-modal resin method called EV-Clean, improvements have been demonstrated in flow cytometric EV analysis.
Review
Chemistry, Analytical
Zheng Zhou, Yao Chen, Shu Zhu, Linbo Liu, Zhonghua Ni, Nan Xiang
Summary: Inertial microfluidics, proposed in 2007, has been extensively studied and applied in the biomedical field, offering advantages such as high throughput, simplicity, and low cost. This technology is promising for rapid sample processing, cell analysis, and detection, playing a crucial role in cellular sample pretreatment.
Review
Chemistry, Analytical
Majood Haddad, Alex N. Frickenstein, Stefan Wilhelm
Summary: Understanding nanoparticle-cell interactions at single-nanoparticle and single-cell resolutions is crucial to improving the design of next-generation nanoparticles for safer, more effective, and more efficient applications in nanomedicine. This review focuses on recent advances in the continuous high-throughput analysis of nanoparticle-cell interactions at the single-cell level, discussing methods such as flow cytometry and inductively coupled plasma mass spectrometry, as well as their intersection in mass cytometry. The review also explores the challenges and opportunities in current single-cell analysis approaches and proposes directions for innovation in high-throughput analysis of nanoparticle-cell interactions.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Physics, Applied
Julie Martin-Wortham, Steffen M. Recktenwald, Marcelle G. M. Lopes, Lars Kaestner, Christian Wagner, Stephan Quint
Summary: The proposed method combines spatial optical modulation and deep learning to detect cells label-free at high throughput while providing their brightfield images. It requires minimal optical components, suitable for lab-on-chip devices, and has been benchmarked with a dataset of red blood cell images.
APPLIED PHYSICS LETTERS
(2021)
Article
Chemistry, Analytical
Gerjen H. Tinnevelt, Kristiaan Wouters, Geert J. Postma, Rita Folcarelli, Jeroen J. Jansen
Summary: White blood cells play a crucial role in protecting the body from diseases, but can also be related to chronic inflammation, auto immune diseases, or leukemia. High-throughput analytical instruments have been developed to measure multiple proteins on millions of single cells to study the identity and function of different white blood cell types. Multivariate statistics are essential for fully extracting the information-rich biochemistry data. The process involves analyzing the study design, formulating a research question, preparing the data, converting single cells into a cellular distribution, and using clustering methods or models for analysis to differentiate cell (sub)types between groups.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Multidisciplinary
Jan Schlegel, Bartlomiej Porebski, Luca Andronico, Leo Hanke, Steven Edwards, Hjalmar Brismar, Ben Murrell, Gerald M. McInerney, Oscar Fernandez-Capetillo, Erdinc Sezgin
Summary: Speed is crucial in dealing with infectious disease outbreaks, including quickly identifying host binding factors to pathogens. The complexity of the host plasma membrane often hinders fast and accurate determination of these binding factors and high-throughput screening for antimicrobial drug targets. This study presents a multiparametric and high-throughput platform that overcomes this barrier, allowing for rapid screening of host binding factors and discovery of new antiviral drug targets. The platform's sensitivity and robustness were demonstrated by successfully blocking SARS-CoV-2 particles using nanobodies and IgGs from human serum samples.
Review
Pharmacology & Pharmacy
Chelsea B. Pratt, Matt Alexander
Summary: The development of CAR-T cells is crucial for the success of adoptive T-cell immunotherapy. While the process of producing autologous CAR-T cells is time-consuming and expensive, the exploration of cost-effective allogeneic CAR-T cells is essential. High-throughput tools are used to monitor the entire process of CAR-T cell design, testing, manufacturing, and patient monitoring to ensure safety and efficacy.
DRUG DISCOVERY TODAY
(2022)
Article
Infectious Diseases
Maude F. Leveque, Delphine Chiffre, Chloe Galtier, Sahar Albaba, Christophe Ravel, Laurence Lachaud, Anais Iemmi, Pierre Flori, Yvon Sterkers
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2019)
Article
Immunology
Corinne Loeuillet, Anais Mondon, Salima Kamche, Veronique Curri, Jean Boutonnat, Pierre Cavailles, Marie-France Cesbron-Delauw
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2019)
Article
Microbiology
Laurence Braun, Marie-Pierre Brenier-Pinchart, Pierre-Mehdi Hammoudi, Dominique Cannella, Sylvie Kieffer-Jaquinod, Julien Voltaire, Veronique Josserand, Bastien Touquet, Yohann Coute, Isabelle Tardieux, Alexandre Bougdour, Mohamed-Ali Hakimi
NATURE MICROBIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Corinne Loeuillet, Bastien Touquet, Jean Francois Guichou, Gilles Labesse, Denis Sereno
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Oncology
Christopher Montemagno, Laurent Dumas, Pierre Cavailles, Mitra Ahmadi, Sandrine Bacot, Marlene Debiossat, Audrey Soubies, Loic Djaileb, Julien Leenhardt, Nicolas De Leiris, Maeva Dufies, Gilles Pages, Sophie Hernot, Nick Devoogdt, Pascale Perret, Laurent Riou, Daniel Fagret, Catherine Ghezzi, Alexis Broisat
Article
Cell Biology
Souad Amiar, Nicholas J. Katris, Laurence Berry, Sheena Dass, Samuel Duley, Christophe-Sebastien Arnold, Melanie J. Shears, Camille Brunet, Bastien Touquet, Geoffrey McFadden, Yoshiki Yamaryo-Botte, Cyrille Y. Botte
Article
Mycology
F. Schein, H. Munoz-Pons, C. Mahinc, R. Grange, P. Cathebras, P. Flori
JOURNAL DE MYCOLOGIE MEDICALE
(2020)
Article
Infectious Diseases
Marie-Laure Gillardie, Oussama Babba, Caroline Mahinc, Maureen Duthel, Claire de Bengy, Clotilde Morineaud, Elisabeth Rivollier, Pierre Flori
Summary: The study evaluated diagnostic methods for urogenital schistosomiasis and found that PCR-based diagnosis is more reliable than traditional microscopic examination. The specificity of antigenic technique is not sufficient for use in low-endemic areas. The results suggest that combining direct microscopic examination with PCR technology can improve the accuracy of diagnosing urogenital schistosomiasis.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Cell Biology
Caroline Cazin, Yasmine Neirijnck, Corinne Loeuillet, Lydia Wehrli, Francoise Kuehne, Isabelle Lordey, Selima Fourati Ben Mustapha, Amin Bouker, Raoudha Zouari, Nicolas Thierry-Mieg, Serge Nef, Christophe Arnoult, Pierre F. Ray, Zine-Eddine Kherraf
Summary: The genetic landscape of male infertility is complex and not all genes highly expressed in testes are essential for spermatogenesis. This study investigates two cases of idiopathic non-obstructive azoospermia and concludes that the genes C1orf185 and CCT6B are likely not involved in NOA and male fertility.
Article
Genetics & Heredity
Corinne Loeuillet, Magali Dhellemmes, Caroline Cazin, Zine-Eddine Kherraf, Selima Fourati Ben Mustapha, Raoudha Zouari, Nicolas Thierry-Mieg, Christophe Arnoult, Pierre F. Ray
Summary: Oocyte maturation defect (OMD) is a common cause of female factor infertility, often caused by ovulatory disorders. Several genes including PATL2, TUBB8, WEE2, and ZP1 have been associated with OMD. This study identified a new frequent ZP1 variant and found degeneration and absence of oocytes in patients with ZP1 mutations.
Article
Biochemistry & Molecular Biology
Delphine Jublot, Pierre Cavailles, Salima Kamche, Denise Francisco, Diana Fontinha, Miguel Prudencio, Jean-Francois Guichou, Gilles Labesse, Denis Sereno, Corinne Loeuillet
Summary: This study reports on the potential of a histone deacetylase inhibitor called JF363, which has antiviral effects against a range of Toxoplasma strains and Plasmodium parasites. The drug administered in vivo delayed the spread of T. gondii infection and controlled the formation of brain cysts in infected mice. Furthermore, the compound showed promising binding efficiency to the active site of T. gondii HDACs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Guillaume Martinez, Charles Coutton, Corinne Loeuillet, Caroline Cazin, Jana Muronova, Magalie Boguenet, Emeline Lambert, Magali Dhellemmes, Genevieve Chevalier, Jean-Pascal Hograindleur, Charline Vilpreux, Yasmine Neirijnck, Zine-Eddine Kherraf, Jessica Escoffier, Serge Nef, Pierre F. Ray, Christophe Arnoult, Jean-Ju Chung
Summary: Male infertility is a significant health concern and high-throughput sequencing has identified gene defects in rare and severe sperm anomalies. However, this study shows that oligogenic defects, accumulation of multiple rare heterozygous variants in different but functionally connected genes, may be responsible for a significant proportion of cases with common and moderate sperm abnormalities. Further research in mice and humans is needed.
Article
Biochemistry & Molecular Biology
Roland Abi Nahed, Magali Dhellemmes, Christine Payre, Emilie Le Blevec, Jean-Philippe Perrier, Sylviane Hennebicq, Jessica Escoffier, Pierre F. Ray, Corinne Loeuillet, Gerard Lambeau, Christophe Arnoult
Summary: The secreted phospholipase A2 (PLA2G10) plays an important role in mouse fertilization by enhancing acrosome reaction and secretion, improving fertilization rate, and binding to PLA2R1 on gametes. It is shown that the catalytic activity and PLA2R1 binding of PLA2G10 both contribute to its profertility effect, and the study provides new insights into the complexity of PLA2G10's action in optimizing fertilization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
