Evidence that cervical cancer cells secrete IL-2, which becomes an autocrine growth factor

We present evidence that cervical cancer cells express a functional IL-2 receptor (IL-2R). In fact, by RTPCR we obtained that the IL-2R is present in CALO, and INBL cells, and that it consisted of the alpha IL-2R, beta IL-2R, and gamma IL-2R chains. We also found that IL-2 is a growth factor for these cell lines, and unexpectedly that CALO and INBL themselves being cancer cells produce, and secrete IL-2. Antibodies against the alpha and beta subunits of the IL-2R inhibited cell proliferation thus hinting to a cell growth dependency on this factor. Our results thus provide evidence that the IL-2R on cervical cancer cells is part of an autocrine mechanism for its growth to the extent that, like lymphocytes, they produce and become partially dependent on this growth factor. We think that in view of our results caution should be taken when IL-2 is being considered for cancer therapy; in particular when the patient's cancer cells present the IL-2R, because as indicated by our results, the use of this factor could promote tumor growth. Finally, the possible implications of the expression of both IL-2, and IL-2R on cervical cancer cells on the immune escape mechanism of tumor cells are discussed. (C) 2010 Elsevier Ltd. All rights reserved.