期刊
CYTOKINE
卷 45, 期 2, 页码 99-104出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.cyto.2008.11.005
关键词
Porphyromonas gingivalis; Interleukin-1 beta; Interleukin-18; Monocytes; Periodontal disease
资金
- MRC [G0501478] Funding Source: UKRI
- Medical Research Council [G0501478] Funding Source: researchfish
- Medical Research Council [G0501478] Funding Source: Medline
Porphyromonas gingivalis is a major bacterial species implicated in chornic periodontitis, a disease characterized by inflammatory destruction of the tooth supporting tissues. Its main virulence factors are lipopolysaccharide (LPS) and gingipains, a group of cysteine proteinases. Interleukin (IL)-18 is a potent pro-inflammatory cytokine with structural similarities to IL-1 beta. This study aimed to investigate if P.gingivalis regulates IL-1 beta and IL-18 in monocytic cells. Monomac-6 cells were challenged with P. gingivalis culture supernatants. Quantitative real-time PCR and ELISA were used to investigate IL-1 beta and IL-18 mRNA expression and protein secretion, respectively. P. gingivalis enhanced IL-1 beta and IL-18 mRNA expression, the former being induced earlier, but transiently. IL-18 up-regulation was not affected by P. gingivalis heat-inactivation or chemical inhibition of its gingipains, whereas both treatments resulted in 50% reduction of IL-1 beta expression. Purified A gingivalis LPS enhanced both IL-1 beta and IL-18 expression. However, only IL-1 beta, but not IL-18, secretion was detected, and was up-regulated by P. gingivalis. In conclusion, although IL-1 beta and IL-18 belong to the same cytokine family, their gene expression and secretion are differentially regulated in human monocytic cells in response to P. gingivalis. Therefore, cytokines of the IL-1 family may participate via different pathways in the complex pathogenesis of periodontitis. (C) 2008 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据