期刊
CYTOGENETIC AND GENOME RESEARCH
卷 126, 期 4, 页码 368-375出版社
KARGER
DOI: 10.1159/000275796
关键词
Evolution; Genomic in situ hybridization; Phylogenetics
资金
- Agency of the Czech Republic [204/09/H002, 522/09/0083, 521/08/0932]
- Czech Ministry of Education [LC 06004]
- Czech Academy of Sciences [AVOZ50040507, AVOZ50040702]
This review summarizes conventional and recent applications of genomic in situ hybridization (GISH). GISH is a well recognized technique, but its modifications and applications have not been widely used. Here, we show how modifications to the GISH technique can be used as tools to 'paint' plant chromosomes. In addition, we describe novel applications, e. g. how GISH banding could be used for karyotyping plant chromosomes. We further discuss recent phylogenetic applications of GISH that allow a semiquantitative signal analysis and the possibility of comparing and combining this cytogenetic technique with DNA sequence-based phylogenetic trees. Copyright (C) 2010 S. Karger AG, Basel
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