期刊
CYTOGENETIC AND GENOME RESEARCH
卷 125, 期 1, 页码 74-80出版社
KARGER
DOI: 10.1159/000218749
关键词
BAC; FISH; Populus trichocarpa; rDNA; Telomere
资金
- U.S. Department of Energy [DE-AC05-00OR22725]
- NSF [0421743]
- Southern Research Station (U.S. Forest Service)
The 18S-28S rDNA and 5S rDNA loci in Populus trichocarpa were localized using fluorescent in situ hybridization ( FISH). Two 18S-28S rDNA sites and one 5S rDNA site were identified and located at the ends of 3 different chromosomes. FISH signals from the Arabidopsis-type telomere repeat sequence were observed at the distal ends of each chromosome. Six BAC clones selected from 2 linkage groups based on genome sequence assembly (LG-I and LG-VI) were localized on 2 chromosomes, as expected. BACs from LG-I hybridized to the longest chromosome in the complement. All BAC positions were found to be concordant with sequence assembly positions. BAC-FISH will be useful for delineating each of the Populus trichocarpa chromosomes and improving the sequence assembly of this model angiosperm tree species. Copyright (C) 2009 S. Karger AG, Basel
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