期刊
CUTANEOUS AND OCULAR TOXICOLOGY
卷 29, 期 2, 页码 122-129出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/15569521003745685
关键词
Deferoxamine mesylate; retinal pigment epithelium; RPE; mitogen-activated kinase; MAPK; p38; Erk
Deferoxamine mesylate is clinically used as a chelating agent but might induce retinopathy. To evaluate its effect on the retinal pigment epithelium (RPE), porcine RPE cells were stimulated with deferoxamine. Cell death was assessed with trypan blue exclusion assay. To investigate the pathway of cell death, the mitogen-activated protein kinases (MAPKs) Erk, JNK, and p38 were inhibited with U0126, SP600125, and SB203580, respectively. Their activity was determined by Western blot. Deferoxamine induces significant cell death in RPE cells, accompanied by phosphorylation of p38 and Erk. Inhibition of p38 attenuates cell death. In conclusion, deferoxamine is directly toxic on RPE cells, its toxicity depending on p38.
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