Review
Engineering, Biomedical
Ziyi Feng, Meiqi Jin, Junzhi Liang, Junning Kang, Huazhe Yang, Shu Guo, Xiaoting Sun
Summary: Bone damage may require bone grafting and bone tissue engineering can be an alternative strategy. Mesenchymal stem cells (MSCs) are important for tissue engineering due to their ability to differentiate into various cell types. The regulation of MSCs differentiation and the role of mitochondria in this process are crucial for efficient bone regeneration.
ACTA BIOMATERIALIA
(2023)
Article
Engineering, Biomedical
Isnayra Kerolaynne Carneiro Pacheco, Fernando Da Silva Reis, Camila Ernanda Sousa De Carvalho, Jose Milton Elias De Matos, Napoleao Martins Argolo Neto, Silvia De Araujo Franca Baeta, Karla Rovaris Da Silva, Hugo Victor Dantas, Frederico Barbosa De Sousa, Ana Cristina Vasconcelos Fialho
Summary: The study successfully produced and characterized castor polyurethane (PU) scaffolds, demonstrating their in vitro biocompatibility with stem cells and in vivo osteoinductive effect on bone repair. The scaffolds exhibited porous structure, absence of cytotoxicity, and good cell adhesiveness.
BIOMEDICAL MATERIALS
(2021)
Article
Chemistry, Medicinal
Farah Alwani Azaman, Margaret E. Brennan E. Fournet, Suzina Sheikh Ab Hamid, Muhamad Syahrul Fitri Zawawi, Valdemiro Amaro da Silva Junior, Declan M. M. Devine
Summary: The field of bone tissue engineering is developing various bone graft substitute materials to reconstruct new bone tissue while maintaining native bone characteristics. Insufficient scaffold degradation is currently the critical limitation for tailoring bone formation turnover rate. This study examines novel scaffold formulations, and results show that the CS/HAp/FAp 1:1 formulation can improve scaffold degradation and bone regeneration, providing a cost-effective alternative to traditional methods (BMP-2).
Article
Cell Biology
Sandra Stamnitz, Agnieszka Krawczenko, Urszula Szalaj, Zaneta Gorecka, Agnieszka Antonczyk, Zdzislaw Kielbowicz, Wojciech Swieszkowski, Witold Lojkowski, Aleksandra Klimczak
Summary: FGF-2 and BMP-2 enhance the osteogenic differentiation potential of MSCs grown on a scaffold, making them promising tools for bone regeneration. This was confirmed through in vitro and in vivo experiments.
Review
Cell Biology
Huan Zhou, Lei Zhang, Yue Chen, Chun-Hui Zhu, Fa-Ming Chen, Ang Li
Summary: Bone formation is a complex regeneration process regulated by multiple signaling pathways, with the Hedgehog (Hh) pathway playing a crucial role. Dysregulation of the Hh pathway in adults is associated with bone-related diseases, while during repair processes, it can be reactivated to modulate bone formation. Current therapeutic strategies targeting the Hh cascade for osteogenesis coordination have been reviewed.
CELL PROLIFERATION
(2022)
Article
Polymer Science
Banafsheh Safari, Marziyeh Aghazadeh, Leila Roshangar, Ayuob Aghanejad, Soodabeh Davaran
Summary: This study reports the fabrication and evaluation of a bioactive hybrid scaffold collagen/P-PCL for bone tissue engineering. The scaffold showed biocompatibility, osteoconductivity, and osteoinductivity, supporting the proliferation and differentiation of AD-MSCs.
EUROPEAN POLYMER JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Daniel Garcia-Sanchez, Alberto Gonzalez-Gonzalez, Itziar Alvarez-Iglesias, Monica del Dujo-Gutierrez, Alfonso Bolado-Carrancio, Matilde Certo, Maria Isabel Perez-Nunez, Jose A. Riancho, Jose Carlos Rodriguez-Rey, Jesus Delgado-Calle, Flor Maria Perez-Campo
Summary: The secretome from MSCs in which Sfrp1 has been silenced shows positive effects on bone regeneration by enhancing osteogenic differentiation, decreasing the expression of Wnt inhibitor Dkk1, and inhibiting osteoclastogenesis. The secretome also increases mineralization in human primary MSCs from osteoporotic patients. Proteomic analysis reveals enrichment of osteogenesis-related proteins in the secretome, suggesting that this approach can enhance the osteo-regenerative properties of MSCs' secretome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Samuel Herberg, Daniel Varghai, Daniel S. Alt, Phuong N. Dang, Honghyun Park, Yuxuan Cheng, Jung-Youn Shin, Anna D. Dikina, Joel D. Boerckel, Marsha W. Rolle, Eben Alsberg
Summary: This study demonstrates the potential of localized morphogen presentation to stimulate chondrogenic priming/endochondral differentiation in engineered hMSC tubes compared to loosely packed hMSC sheets for defect healing. hMSC tubes, unlike hMSC sheets, induced regenerate tissue partially resembling normal growth plate in long bone regeneration.
COMMUNICATIONS BIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Shaoyang Ma, Yuchen Zhang, Sijia Li, Ang Li, Ye Li, Dandan Pei
Summary: Bone defect repair is a challenging clinical problem, but engineering exosomes have the potential to overcome the limitations of natural exosomes. By regulating their production and function, engineering exosomes can be a solution for bone defect repair.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Shuqin Zhang, Denghui Xie, Qun Zhang
Summary: This review summarizes the current status and prospects of bone regeneration therapy, introducing the roles of scaffolds and stem cells in tissue engineering, common treatment strategies for bone metabolism-related diseases, and the direction for further development.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Chen Yang, Hongshi Ma, Zhiyong Wang, Muhammad Rizwan Younis, Chunyang Liu, Chengtie Wu, Yongxiang Luo, Peng Huang
Summary: This study presents a novel multifunctional bone scaffold integrating a NIR-II photothermal conversion agent with tremendous bioactivity with polycaprolactone via 3D printing. The scaffold triggers osteosarcoma ablation and promotes cellular proliferation and osteogenic differentiation, leading to enhanced vascularized bone regeneration in vivo. This research provides a new approach for the development of therapeutic bioceramic-based multifunctional bone scaffolds for repairing tumor-induced bone defects.
Article
Materials Science, Biomaterials
Xiongcheng Xu, Long Xiao, Yanmei Xu, Jin Zhuo, Xue Yang, Li Li, Nianqi Xiao, Jing Tao, Quan Zhong, Yanfen Li, Yuling Chen, Zhibin Du, Kai Luo
Summary: The study developed a PCL/LAP scaffold with excellent bioactivity using 3D printing technology, promoting osteogenic differentiation and angiogenesis of bone marrow mesenchymal stem cells, resulting in significant enhancement of vascularized bone formation in a calvarial defect model.
REGENERATIVE BIOMATERIALS
(2021)
Review
Cell Biology
Kangkang Zha, Yue Tian, Adriana C. Panayi, Bobin Mi, Guohui Liu
Summary: Bone tissue engineering offers a new approach to treating bone defects, with mesenchymal stem cells being a promising seed cell due to their functions in osteogenic differentiation. Improving methods of origin selection, culture conditions, and biophysical stimulation can enhance MSC osteogenesis and contribute to the development of MSC-based bone tissue engineering.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Engineering, Biomedical
Liaobing Xin, Xiaowen Zheng, Jianmin Chen, Sentao Hu, Yilun Luo, Qunzi Ge, Xiaoying Jin, Lie Ma, Songying Zhang
Summary: This study explores an acellular biomaterial called SDF-1 alpha/E7-modified collagen scaffold (CES), which enables near-complete endometrium regeneration and fertility restoration by mobilizing endogenous MSCs. The results demonstrate that CES can overcome the traditional limitations of cell-based therapies and serve as a clinically feasible and cell-free strategy with high therapeutic efficiency for IUA treatment.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Engineering, Biomedical
Shuangpeng Jiang, Guangzhao Tian, Zhen Yang, Xiang Gao, Fuxin Wang, Juntan Li, Zhuang Tian, Bo Huang, Fu Wei, Xinyu Sang, Liuqi Shao, Jian Zhou, Zhenyong Wang, Shuyun Liu, Xiang Sui, Quanyi Guo, Weimin Guo, Xu Li
Summary: This study confirms that hWJMSC-Exos can enhance the effect of the ACECM scaffold and promote osteochondral regeneration. The promoting effect may be related to the polarization of macrophages and inhibition of inflammatory response by hWJMSC-Exos. Additionally, hWJMSC-Exos contain miRNAs that can promote hyaline cartilage regeneration.
BIOACTIVE MATERIALS
(2021)
Article
Critical Care Medicine
Enrique Gomez-Barrena, Norma Padilla-Eguiluz, Philippe Rosset, Florian Gebhard, Philippe Hernigou, Nicola Baldini, Helene Rouard, Luc Sensebe, Rosa-Maria Gonzalo-Daganzo, Rosaria Giordano, Eduardo Garcia-Rey, Jose Cordero-Ampuero, Juan Carlos Rubio-Suarez, Marta Dominguez Garcia-Simon, Julien Stanovici, Christian Ehrnthaller, Markus Huber-Lang, Charles Henri Flouzat-Lachaniette, Nathalie Chevallier, Davide Maria Donati, Benedetta Spazzoli, Gabriela Ciapetti, Sandrine Fleury, Manuel-Nicolas Fernandez, Jose-Rafael Cabrera, Cristina Avendano-Sola, Tiziana Montemurro, Carmen Panaitescuk, Elena Veronesi, Markus Thomas Rojewskit, Ramin Lotfi, Massimo Dominici, Hubert Schrezenmeier, Pierre Layrolle
INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED
(2020)
Review
Medicine, Research & Experimental
Thomas G. P. Grunewald, Marta Alonso, Sofia Avnet, Ana Banito, Stefan Burdach, Florencia Cidre-Aranaz, Gemma Di Pompo, Martin Distel, Heathcliff Dorado-Garcia, Javier Garcia-Castro, Laura Gonzalez-Gonzalez, Agamemnon E. Grigoriadis, Merve Kasan, Christian Koelsche, Manuela Krumbholz, Fernando Lecanda, Silvia Lemma, Dario L. Longo, Claudia Madrigal-Esquivel, Alvaro Morales-Molina, Julian Musa, Shunya Ohmura, Benjamin Ory, Miguel Pereira-Silva, Francesca Perut, Rene Rodriguez, Carolin Seeling, Nada Al Shaaili, Shabnam Shaabani, Kristina Shiavone, Snehadri Sinha, Eleni M. Tomazou, Marcel Trautmann, Maria Vela, Yvonne M. H. Versleijen-Jonkers, Julia Visgauss, Marta Zalacain, Sebastian J. Schober, Andrej Lissat, William R. English, Nicola Baldini, Dominique Heymann
EMBO MOLECULAR MEDICINE
(2020)
Article
Chemistry, Analytical
Francesco Costa, Luigi Falzetti, Nicola Baldini, Sofia Avnet
Article
Chemistry, Multidisciplinary
Giorgia Montalbano, Giorgia Borciani, Giorgia Cerqueni, Caterina Licini, Federica Banche-Niclot, Davide Janner, Stefania Sola, Sonia Fiorilli, Monica Mattioli-Belmonte, Ciapetti Gabriela, Chiara Vitale-Brovarone
Article
Materials Science, Biomaterials
Rita Gelli, Gemma Di Pompo, Gabriela Graziani, Sofia Avnet, Nicola Baldini, Piero Baglioni, Francesca Ridi
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2020)
Article
Nutrition & Dietetics
Francesca Perut, Gabriela Graziani, Marta Columbaro, Renata Caudarella, Nicola Baldini, Donatella Granchi
Article
Oncology
MargheritaSA Cortini, Andrea Armirotti, Marta Columbaro, Dario Livio Longo, Gemma Di Pompo, Elena Cannas, Alessandra Maresca, Costantino Errani, Alessandra Longhi, Alberto Righi, Valerio Carelli, Nicola Baldini, Sofia Avnet
Summary: This study identified a novel lipid signaling pathway selectively activated in acid-induced metastatic osteosarcoma cells, and demonstrated the impairment of tumor growth by targeting this pathway with FTY720 in combination with a low-serine/glycine diet. The high circulating levels of S1P in osteosarcoma patients suggest its potential as a biomarker.
Article
Biochemistry & Molecular Biology
Francesca Perut, Laura Roncuzzi, Sofia Avnet, Annamaria Massa, Nicoletta Zini, Silvia Sabbadini, Francesca Giampieri, Bruno Mezzetti, Nicola Baldini
Summary: EPDENs isolated from strawberries have been shown to have potential health-promoting activities, including preventing oxidative stress in human mesenchymal stromal cells. The presence of vitamin C in the EPDEN membrane may contribute to this effect, along with the small RNAs and miRNAs found in the cargo. These findings suggest that Fragaria-derived EPDENs may serve as nanoshuttles in food with bioactive properties.
Article
Medicine, General & Internal
Enrique Gomez-Barrena, Norma G. Padilla-Eguiluz, Philippe Rosset, Philippe Hernigou, Nicola Baldini, Gabriela Ciapetti, Rosa M. Gonzalo-Daganzo, Cristina Avendano-Sola, Helene Rouard, Rosaria Giordano, Massimo Dominici, Hubert Schrezenmeier, Pierre Layrolle
Summary: The study demonstrated the safety and early efficacy of treating early femoral head osteonecrosis with autologous mesenchymal stromal cells (MSC) expanded from bone marrow. Results showed that 80% of patients maintained head sphericity and showed bone regeneration at 2 years, with 19% converting to total hip replacement (THR) after 5 years.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Cell Biology
Giorgia Borciani, Giorgia Montalbano, Priscila Melo, Nicola Baldini, Gabriela Ciapetti, Chiara Vitale-Brovarone
Summary: Osteoporosis is a global disease that increases bone fragility and fracture risk in adults. Bone tissue engineering, which combines biomaterials, cells, and other factors, is being explored as an alternative treatment. In this study, two composite materials were developed and tested with human bone-derived osteoblasts and osteoclast precursors, demonstrating their potential as biomaterial inks for 3D printing complex scaffolds for bone tissue engineering.
Article
Cell & Tissue Engineering
Nicola Rani, Francesca Perut, Donatella Granchi, Giuseppe Di Sante, Enrico Pennello, Alessandro Mazzotta, Dante Dallari, Nicola Baldini
Summary: This study compared the ability of autologous platelet-rich plasma (PRP) and cord blood PRP (PRPc) to accelerate bone healing. The results showed similar bone consolidation between PRP and PRPc treatments, suggesting that PRPc is a valid alternative when clinical conditions discourage the use of autologous blood derivates.
REGENERATIVE MEDICINE
(2022)
Review
Chemistry, Physical
Giorgia Borciani, Gabriela Ciapetti, Chiara Vitale-Brovarone, Nicola Baldini
Summary: Strontium (Sr) is a trace element that can promote bone formation and inhibit bone resorption. Due to the negative cardiac effects of strontium ranelate (SrRan), researchers have started to incorporate Sr into biomaterials for bone tissue engineering. In vitro and in vivo studies have shown that the addition of Sr in functionalized materials can enhance bone formation and reduce osteoclast activity.
Article
Biochemical Research Methods
Giorgia Borciani, Giorgia Montalbano, Nicola Baldini, Chiara Vitale-Brovarone, Gabriela Ciapetti
Summary: This study proposes a clear protocol for setting up an indirect co-culture system of human-derived osteoblasts and osteoclast precursors, which is useful for testing new biomaterials and scaffolds in bone tissue engineering applications.
METHODS AND PROTOCOLS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Valentina Spicciarelli, Crystal Marruganti, Massimo Viviano, Nicola Baldini, Giovanni Franciosi, Mario Tortoriello, Marco Ferrari, Simone Grandini
JOURNAL OF ORAL SCIENCE
(2020)
Article
Dentistry, Oral Surgery & Medicine
V Spicciarelli, C. Marruganti, M. Viviano, N. Baldini, G. Franciosi, M. Tortoriello, S. Grandini
JOURNAL OF OSSEOINTEGRATION
(2020)