期刊
CURRENT OPINION IN STRUCTURAL BIOLOGY
卷 23, 期 3, 页码 409-416出版社
CURRENT BIOLOGY LTD
DOI: 10.1016/j.sbi.2013.03.003
关键词
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资金
- Swiss National Center of Competence in Research (NCCR) Structural Biology
Novel tools and technologies are required to obtain structural information of difficult to crystallize complex biological systems such as membrane proteins, multiprotein assemblies, transient conformational states and intrinsically disordered proteins. One promising approach is to select a high affinity and specificity-binding partner (crystallization chaperone), form a complex with the protein of interest and crystallize the complex. Often the chaperone reduces the conformational freedom of the target protein and additionally facilitates the formation of well-ordered crystals. This review provides an update on the recent successes in chaperone-assisted crystallography. We also stress the importance of synergistic approaches involving protein engineering, crystallization chaperones and crystallization additives. Recent examples demonstrate that investment in such approaches can be key to success.
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