Article
Biology
Kelvin W. Pond, Julia M. Morris, Olga Alkhimenok, Reeba P. Varghese, Carly R. Cabel, Nathan A. Ellis, Jayati Chakrabarti, Yana Zavros, Juanita L. Merchant, Curtis A. Thorne, Andrew L. Paek
Summary: The establishment and maintenance of different cellular compartments in tissues is crucial for all metazoans, especially in the human colon. This study reveals the critical role of wave-like ERK signaling induced by apoptotic cells in maintaining tissue patterning and homeostasis.
Article
Multidisciplinary Sciences
Alexander Plong, Kevin Rodriguez, Mark Alber, Weitao Chen, G. Venugopala Reddy
Summary: WUSCHEL concentration regulation is crucial for stem cell homeostasis in Arabidopsis shoot apical meristems. CLAVATA3 not only represses WUSCHEL transcription, but also regulates WUSCHEL by controlling its nuclear export and diffusion between adjacent cells.
NATURE COMMUNICATIONS
(2021)
Article
Biochemical Research Methods
Takaki Yamamoto, Katie Cockburn, Valentina Greco, Kyogo Kawaguchi
Summary: This article demonstrates the potential application of graph neural network (GNN) models in predicting cell fate and inferring cell interactions in tissues. By analyzing live mammalian epidermis data, GNN discovers neighbor cell fate coordination rules that vary across different regions of the body.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Plant Sciences
Yongdong Yu, Zhaochen Zhong, Liuyin Ma, Chengbin Xiang, Jie Chen, Xin-Yuan Huang, Ping Xu, Yan Xiong
Summary: Plants play a crucial role in the global sulfur cycle and their growth is regulated by the sulfate-TOR signal pathway. Activation of TOR by inorganic sulfate promotes true leaf development in Arabidopsis independently of sulfate-assimilation and glucose-energy signaling. The TOR signaling pathway controls DNA replication, cell proliferation, and secondary metabolism pathways, and responds to different nutrient signals to precisely modulate plant growth and stress responses.
Article
Biochemistry & Molecular Biology
Yukun Wang, Makoto Shirakawa, Toshiro Ito
Summary: This study reveals the dynamic changes in stem cell death and activity during the life cycle of Arabidopsis thaliana, as well as the role of reactive oxygen species in this process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Baojian Chen, Martijn Fiers, Bas J. W. Dekkers, Lena Maas, G. Wilma van Esse, Gerco C. Angenent, Yang Zhao, Kim Boutilier
Summary: Research shows that exposing explants to growth regulator 2,4-D can induce somatic embryogenesis in Arabidopsis seeds, with a crucial role played by ABA in promoting this process by maintaining the seed maturation environment and regulating the expression of transcription factors.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Zichen Kang, Hikari Matsumoto, Tomonobu Nonoyama, Sakumi Nakagawa, Yukitaka Ishimoto, Satoru Tsugawa, Minako Ueda
Summary: Polarization and elongation of the zygote determine the body axis in plant development. Based on live-cell imaging, we observed that the Arabidopsis zygote elongates only at its apical tip, which retains a hemispherical shape. The growth pattern of the zygote also changes with developmental stage.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Plant Sciences
Zhanchao Cheng, Changhong Mu, Xiangyu Li, Wenlong Cheng, Miaomiao Cai, Chongyang Wu, Jutang Jiang, Hui Fang, Yucong Bai, Huifang Zheng, Ruiman Geng, Junlei Xu, Yali Xie, Yuping Dou, Juan Li, Shaohua Mu, Jian Gao
Summary: In this study, a modified protocol for protoplast preparation was used to investigate the single-cell transcriptome of the root system in moso bamboo. Four cell types were identified, and new cell-type-specific marker genes for the basal root were defined. The developmental trajectories of the root cap, epidermis, and ground tissues were reconstructed, and key factors regulating cell fate determination were elucidated. Furthermore, the study revealed the divergence of root developmental pathways between moso bamboo and rice and identified key regulatory factors for cell differentiation.
HORTICULTURE RESEARCH
(2023)
Article
Plant Sciences
Yuki Hirakawa
Summary: CLE peptides control stem cell activity in meristems, with some CLE peptides promoting stem cells and others suppressing them. The CLV3 pathway, involving a negative feedback loop, plays a crucial role in maintaining stem cell population and regulating shoot growth and organogenesis. This paper proposes a model for the evolution of meristem zonation based on recent studies, suggesting that a stem cell limiting pathway evolved from a stem cell promoting pathway through gene duplication in angiosperms. Future genomic and developmental studies can verify this model.
Article
Biology
Dennis May, Sangwon Yun, David G. Gonzalez, Sangbum Park, Yanbo Chen, Elizabeth Lathrop, Biao Cai, Tianchi Xin, Hongyu Zhao, Siyuan Wang, Lauren E. Gonzalez, Katie Cockburn, Valentina Greco
Summary: Stem cell differentiation involves dramatic changes in gene expression and global remodeling of chromatin architecture. In this study, a quantitative pipeline using fluorescently-tagged histones and longitudinal imaging was developed to track chromatin compaction changes in live mice. Analysis of epidermal stem cells reveals that cell-to-cell chromatin compaction heterogeneity is independent of cell cycle status and reflective of differentiation status. Furthermore, live imaging of Keratin-10 nascent RNA shows that transcription precedes global chromatin compaction changes during differentiation. These findings highlight the dynamic transcriptional states and gradual chromatin rearrangement involved in stem cell differentiation.
Article
Developmental Biology
Liang Zhang, Daniel DeGennaro, Guangzhong Lin, Jijie Chai, Elena D. Shpak
Summary: The shoot apical meristem is a crucial reservoir of stem cells in plants, with its size regulated by a balance of stem cell replenishment and cell incorporation into organ primordia. The WUS/CLV negative feedback loop plays a central role in SAM size regulation, depending on accurate spatial expression of WUS and CLV3. A signaling pathway involving ERf receptors and EPFL ligands restricts SAM width and promotes leaf initiation by inhibiting the expression of WUS and CLV3 in the periphery and confining them to the center of the SAM.
Article
Developmental Biology
Gema Villa-Fombuena, Maria Lobo-Pecellin, Miriam Marin-Menguiano, Patricia Rojas-Rios, Acaimo Gonzalez-Reyes
Summary: By studying Drosophila female germline stem cells, researchers found that the spectrosomes within GSCs and cystoblasts undergo shape changes depending on the cell cycle phase and are essential for spindle orientation during mitosis. The asymmetric division of GSCs is influenced by the niche environment, as demonstrated by symmetric division of GSC-like cells located outside the niche.
Article
Developmental Biology
Thai Q. Dao, Naama Weksler, Hannah M-H Liu, Samuel Leiboff, Jennifer C. Fletcher
Summary: The study identifies two CLV3-related peptides, CLE16 and CLE17, in Arabidopsis thaliana plants, which play a role in maintaining shoot apical meristem and organ production. They regulate the expression of WUS through specific receptors and signaling pathways. These findings are important for understanding the regulation mechanisms of plant stem cell activity.
Article
Plant Sciences
Sylvia R. Silveira, Constance Le Gloanec, Andrea Gomez-Felipe, Anne-Lise Routier-Kierzkowska, Daniel Kierzkowski
Summary: Development of multicellular organisms requires precise coordination among individual cells. In this study, a confocal time-lapse imaging method was used to quantitatively characterize the development of stamens in Arabidopsis. The study revealed early specification of the filament and anther, as well as three distinct phases of filament development. This research provides a quantitative atlas for analyzing cellular growth dynamics in stamen organogenesis.
Review
Biochemistry & Molecular Biology
Leia Colin, Raquel Martin-Arevalillo, Simone Bovio, Amelie Bauer, Teva Vernoux, Marie-Cecile Caillaud, Benoit Landrein, Yvon Jaillais
Summary: Biological imaging is an interdisciplinary field crucial in cell biology research, involving techniques, probe development, and image analysis. Recent advances in live imaging of plant cells include studies on cell structure, hormones, mechanical properties, etc. driving the field forward.
