Review
Immunology
Jiale Li, Qinbao Peng, Ronghua Yang, Kunsheng Li, Peng Zhu, Yufeng Zhu, Pengyu Zhou, Gabor Szabo, Shaoyi Zheng
Summary: This review discusses the use of machine perfusion and mesenchymal stem cells to improve the quality and outcomes of marginal organs in transplantation. By continuously perfusing the organs to mimic physiological conditions, evaluating pre-transplant organ function, and treating the organs with pharmacological, gene, and stem cell therapy, the quality and adaptability of the organs can be enhanced. Promising preclinical results indicate the potential clinical translation of this innovative strategy to improve the quality of marginal grafts.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Adam Hosszu, Akos R. R. Toth, Tamas Lakat, Ganna Stepanova, Zsuzsanna Antal, Laszlo J. J. Wagner, Attila J. J. Szabo, Andrea Fekete
Summary: Kidney transplantation is the preferred treatment for end-stage kidney disease, and optimizing organ condition is crucial due to the shortage of transplantable organs. This study demonstrated that supplementing the preservation solution with the S1R agonist fluvoxamine reduced cold and warm ischemic injury, improved kidney function, mitigated histological injury, and reduced inflammation. The results suggest that S1R agonists have great potential in improving organ preservation and alleviating the problem of organ shortages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Pharmacology & Pharmacy
Min Cao, Guoqing Wang, Hongli He, Ruiming Yue, Yong Zhao, Lingai Pan, Weiwei Huang, Yang Guo, Tao Yin, Lina Ma, Dingding Zhang, Xiaobo Huang
Summary: The transplant community is still striving to improve graft quality and recipient prognosis, with a focus on enhancing oxygenation during organ preservation. The addition of HBOCs to traditional preservation protocols has shown promise in providing more oxygen to organs, reducing ischemia-reperfusion injury, and increasing the pool of transplantable donors.
FRONTIERS IN PHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Maria Irene Bellini, Francesco Tortorici, Maria Ida Amabile, Vito D'Andrea
Summary: Kidney transplantation is the preferred treatment for end-stage renal disease, with static cold storage being the standard preservation method. However, dynamic techniques like machine perfusion have shown potential to improve graft function and quality assessment, particularly in kidneys from extended criteria donors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ophelie Pasini-Chabot, Julia Vincent, Sylvain Le Pape, Maryne Lepoittevin, Wassim Kaaki, Jean-Baptiste Woillard, Sebastien Giraud, Nicolas Bourmeyster, Thierry Hauet, Raphael Thuillier
Summary: The study indicates that significant changes in protein expression occur in key pathways during organ preservation, with time-sensitive protein expression potentially serving as quality biomarkers for organs and targets for new strategies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Pharmacology & Pharmacy
Chenxi Lu, Changyu Wang, Haoxiang Xiao, Mengfan Chen, Zhi Yang, Zhenxing Liang, Haiying Wang, Yonglin Liu, Yang Yang, Qiang Wang
Summary: Ischemia-reperfusion injury (IRI) is a process characterized by innate immune response and changes in the structure and function of multiple organs. Ethyl pyruvate (EP), a derivative of pyruvate, has gained attention for its stability and low toxicity, with various pharmacological activities such as anti-inflammation, anti-oxidative stress, anti-apoptosis, and anti-fibrosis. EP has shown beneficial effects in acute injury models and has the potential for therapeutic intervention in multiple organ IRI.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Reinier J. de Vries, Stephanie E. J. Cronin, Padraic Romfh, Casie A. Pendexter, Rohil Jain, Benjamin T. Wilks, Siavash Raigani, Thomas M. van Gulik, Peili Chen, Heidi Yeh, Korkut Uygun, Shannon N. Tessier
Summary: The study used resonance Raman spectroscopy to provide a rapid, non-invasive, label-free diagnostic for quantifying hepatic mitochondrial redox status, showing its potential in distinguishing transplantable versus non-transplantable ischemically injured rat livers and revealing spatial differences in mitochondrial redox response to ischemia reperfusion. This novel diagnostic could be helpful in predicting the viability of human livers for transplantation and furthering understanding of hepatic IRI mechanisms.
Article
Multidisciplinary Sciences
Yong Gyu Lee, Jung-Lye Kim, Andre F. Palmer, Brenda F. Reader, Jianjie Ma, Sylvester M. Black, Bryan A. Whitson
Summary: Based on our experience with rat lung transplantation, varying methods for choosing appropriate cuff sizes for the PV, PA, and Br have been found. Standardizing the cuffing technique to use the smallest effective cuff suitable for vessel or bronchus size is recommended for safer and more successful procedures. Strategies for size selection based on weight and consideration of the relationship between weight and lung volume are also suggested.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Cardiac & Cardiovascular Systems
Etienne Abdelnour-Berchtold, Aadil Ali, Cristina Baciu, Erika L. Beroncal, Aizhou Wang, Olivia Hough, Mitsuaki Kawashima, Manyin Chen, Yu Zhang, Mingyao Liu, Tom Waddell, Ana C. Andreazza, Shaf Keshavjee, Marcelo Cypel
Summary: Injured donor lungs stored at 10℃ demonstrated superior oxygenation and protective effects compared to the current clinical standard of 4℃ storage.
JOURNAL OF HEART AND LUNG TRANSPLANTATION
(2022)
Article
Biochemistry & Molecular Biology
Hanno Maassen, Leonie H. Venema, Marc G. Weiss, Tobias M. Huijink, H. Sijbrand Hofker, Anna K. Keller, Tom E. Mollnes, Marco Eijken, Soren E. Pischke, Bente Jespersen, Harry van Goor, Henri G. D. Leuvenink
Summary: Kidney extraction time has a detrimental effect on post-transplantation outcome. This study aimed to improve the flush-out and decrease ischemic injury by adding hydrogen sulphide (H2S) to the flush medium. The addition of H2S during flush out and static cold storage resulted in a reduction in pro-inflammatory cytokines without affecting renal function or injury markers.
Article
Pharmacology & Pharmacy
Roua Chaabani, Mohamed Bejaoui, Mohamed Amine Zaouali, Hassen Ben Abdennebi
Summary: This study evaluated the effect of adding diclofenac to the preservation solution Celsior on liver graft preservation. The study found that enriching Celsior with diclofenac significantly reduced liver injuries, oxidative stress, inflammation, and apoptosis, while improving graft function. Diclofenac also activated PPAR-γ and inhibited NF-κB transcription factors. Overall, diclofenac sodium salt may be a promising additive to preservation solution to reduce graft damage and improve transplant recovery.
CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Fan Deng, Ze-Bin Lin, Qi-Shun Sun, Yue Min, Yue Zhang, Yu Chen, Wen-Ting Chen, Jing-Juan Hu, Ke-Xuan Liu
Summary: Intestinal ischemia/reperfusion (I/R) is a common pathological process in severely ill patients, and the impact of intestinal I/R injury on the overall systemic state of the patient is greater than that of primary intestinal injury. Recent evidence suggests that intestinal microbiota and its metabolites play a crucial role in the occurrence, development, diagnosis, and treatment of intestinal I/R injury. Targeting the intestinal microbiota and its metabolites may be an effective therapy for treating intestinal I/R injury and associated extraintestinal organ injuries.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Review
Cell Biology
Marc Mico-Carnero, Mohamed Amine Zaouali, Carlos Rojano-Alfonso, Cristina Maroto-Serrat, Hassen Ben Abdennebi, Carmen Peralta
Summary: Ischemia and reperfusion injury (IRI), an inevitable step in organ transplantation, can cause serious organ damage. Most organs used for transplantation today come from extended criteria donors (ECDs), which are more sensitive to IRI. The main objective of organ preservation solutions is to protect against IRI through specific components.
Review
Gastroenterology & Hepatology
Mauricio Flores Carvalho, Yuri L. Boteon, James V. Guarrera, Pranjal R. Modi, Laura Llado, Georg Lurje, Mureo Kasahara, Philipp Dutkowski, Andrea Schlegel
Summary: This article discusses the challenges of implementing machine perfusion of solid organs in practice, including the roles of clinical practitioners, hospitals, regulatory bodies, and industry stakeholders worldwide. It emphasizes the need for strong collaboration between these stakeholders and presents integrated roadmaps to achieve wider implementation. The importance of research development, clear regulatory pathways, and flexible reimbursement schemes is highlighted, along with potential solutions to address relevant hurdles. The article provides an overview of the current landscape of liver perfusion and underscores the role of clinical, regulatory, and financial stakeholders globally.
Review
Biochemistry & Molecular Biology
Beatrice Lukenaite, Erika Griciune, Bettina Leber, Kestutis Strupas, Philipp Stiegler, Peter Schemmer
Summary: Ischemia-reperfusion injury (IRI) is a common problem during solid organ transplantation (SOT), characterized by multifactorial inflammatory responses. Mixed-lineage kinase domain-like pseudokinase (MLKL) and receptor-interacting protein kinase 3 (RIPK3) play crucial roles in necroptosis. Necrosis also triggers sterile inflammation, leading to graft failure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)