期刊
JOURNAL OF NEUROSCIENCE
卷 35, 期 28, 页码 10357-10370出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.1249-15.2015
关键词
axon regeneration; RNA transport; spinal cord injury; translation
资金
- Craig H. Nielsen Foundation [224125]
- National Institutes of Health [P01-NS055976]
- Dr. Miriam and Sheldon G. Adelson Medical Research Foundation
- South Carolina SmartState Endowment Program (Center for Childhood Neurotherapeutics) through the University of South Carolina
Although intra-axonal protein synthesis is well recognized in cultured neurons and during development in vivo, there have been few reports of mRNA localization and/or intra-axonal translation in mature CNS axons. Indeed, previous work indicated that mature CNS axons contain much lower quantities of translational machinery than PNS axons, leading to the conclusion that the capacity for intra-axonal protein synthesis is linked to the intrinsic capacity of a neuron for regeneration, with mature CNS neurons showing much less growth after injury than PNS neurons. However, when regeneration by CNS axons is facilitated, it is not known whether the intra-axonal content of translational machinery changes or whether mRNAs localize into these axons. Here, we have used a peripheral nerve segment grafted into the transected spinal cord of adult rats as a supportive environment for regeneration by ascending spinal axons. By quantitative fluorescent in situ hybridization combined with immunofluorescence to unambiguously distinguish intra-axonalmRNAs, we show that regenerating spinal cord axons contain beta-actin, GAP-43, Neuritin, Reg3a, Hamp, and Importin beta 1 mRNAs. These axons also contain 5S rRNA, phosphorylated S6 ribosomal protein, eIF2 alpha translation factor, and 4EBP1 translation factor inhibitory protein. Different levels of these mRNAs in CNS axons from regenerating PNS axons may relate to differences in the growth capacity of these neurons, although the presence of mRNA transport and likely local translation in both CNS and PNS neurons suggests an active role in the regenerative process.
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