Article
Hematology
Gaukhar Baidildinova, Vincent ten Cate, Markus Nagler, Marina Panova-Noeva, Steffen Rapp, Thomas Koeck, Juergen H. Prochaska, Stefan Heitmeier, Christoph Gerdes, Stephan Schwers, Stavros V. Konstantinides, Thomas Muenzel, Christine Espinola-Klein, Karl J. Lackner, Henri M. N. Spronk, Hugo ten Cate, Paola E. J. van der Meijden, Kirsten Leineweber, Philipp S. Wild, Kerstin Jurk
Summary: The study reveals specific plasma signatures and differential release mechanisms of proteins related to platelets in acute isolated PE and DVT-associated PE.
THROMBOSIS RESEARCH
(2022)
Editorial Material
Cell Biology
Joseph E. Aslan
Summary: Proteomics tools have greatly contributed to our understanding of platelet biology, with the ability to identify, detect, and quantify protein-related details. The platelet proteome is composed of thousands of different proteins, and changes in this proteome can affect platelet function in health and disease. Challenges still remain in conducting, validating, and interpreting platelet proteomics experiments, but future studies using advanced proteomics technologies may shed more light on platelet-mediated mechanisms in human health and disease.
Review
Biochemistry & Molecular Biology
Meletia Kallianta, Eftychia Pappa, Heleni Vastardis, Christos Rahiotis
Summary: Mass Spectrometry (MS) is a highly sensitive and universal analytical technique that has rapidly developed in recent decades. It has various applications in dentistry, including research on dental materials and agents, laboratory analysis of biospecimens, and real-time diagnostics in oral surgery and pathology. MS can provide valuable insights into the clinical behavior, toxicity, antimicrobial properties, and biomarkers of dental materials and agents. It is also effective in detecting biomarkers in saliva and providing a comprehensive understanding of cellular functions through metabolite analysis. Additionally, MS shows promising potential for surgical approaches to oral malignant disorders. Overall, MS has revolutionized dental research and has the potential to advance personalized dentistry.
Article
Hematology
Iris C. Kreft, Elise J. Huisman, Marjon H. Cnossen, Floris P. J. van Alphen, Carmen van der Zwaan, Karin van Leeuwen, Rosalina van Spaendonk, Leendert Porcelijn, Caroline S. B. Veen, Maartje van den Biggelaar, Masja de Haas, Alexander B. Meijer, Arie J. Hoogendijk
Summary: This study profiles the platelet proteomics signatures of inherited platelet disorders (IPDs) using mass spectrometry analysis. The results demonstrate the extensive proteomic alterations caused by different genetic variants, providing insights into the biology of platelet (dys)function.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2023)
Review
Biochemistry & Molecular Biology
Olga Shevchuk, Antonija Jurak Begonja, Stepan Gambaryan, Matthias Totzeck, Tienush Rassaf, Tobias B. Huber, Andreas Greinacher, Thomas Renne, Albert Sickmann
Summary: This review discusses the role of proteomics in understanding the molecular basics of traditional and emerging functions of platelets, as well as the application of proteomic technologies in platelet biogenesis, signaling, and storage. Additionally, the potential of newly appeared trapped ion mobility spectrometry (TIMS) in platelet research is highlighted, along with discussions on the implementation of proteomic methods in platelet transfusion medicine, diagnostics, and prognostics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Hematology
Peter Schubert, Lacey Johnson, Brankica Culibrk, Zhongming Chen, Shereen Tan, Denese C. Marks, Dana Devine
Summary: Cryo-preserved platelets are capable of synthesizing proteins upon reconstitution and storage, with a subset of these proteins observed in platelet microvesicles (PMVs). This discovery suggests a possible role in vesicle encapsulation, providing insights into protein synthesis and packaging regulation in cryo-PLTs.
Article
Biochemistry & Molecular Biology
Sumaiya Nazli, Kip D. Zimmerman, Angelica M. Riojas, Laura A. Cox, Michael Olivier
Summary: The proteomic analysis of plasma has great potential for precision medicine and biomarker identification. However, detecting and quantifying low abundance proteins in plasma is challenging. This study developed a mass spectrometry approach using tandem mass tags (TMT) and a tissue reference sample, which successfully identified and quantified numerous proteins in nonhuman primate plasma samples. The methodology promises to increase the number of putative disease biomarkers by efficiently quantifying plasma proteins released from specific tissues.
Review
Biochemistry & Molecular Biology
Ekaterina Mikhailovna Stakhneva, Evgeniia Vitalievna Striukova, Yulia Igorevna Ragino
Summary: Proteomic studies play a crucial role in investigating atherosclerotic cardiovascular diseases, identifying potential biomarkers, and offering novel diagnostic approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Larissa van der Maas, Maarten Danial, Gideon F. A. Kersten, Bernard Metz, Hugo D. Meiring
Summary: A mass spectrometric method was developed to identify and quantify antigens in DTaP vaccines. The method can be used for both final batch release and in-process control monitoring, potentially reducing the need for animal testing.
Article
Multidisciplinary Sciences
John P. McGee, Pei Su, Kenneth R. Durbin, Michael A. R. Hollas, Nicholas W. Bateman, G. Larry Maxwell, Thomas P. Conrads, Ryan T. Fellers, Rafael D. Melani, Jeannie M. Camarillo, Jared O. Kafader, Neil L. Kelleher
Summary: This study introduces a semi-automated single-ion mass spectrometry workflow for top-down tandem mass spectrometry analysis directly from tissue microenvironments. The results demonstrate that this method can identify multiple proteoforms in tumor samples within a short period of time, and effectively identify proteoform signatures in tumor and stromal regions.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Nicolai Bjodstrup Palstrom, Rune Matthiesen, Lars Melholt Rasmussen, Hans Christian Beck
Summary: The human plasma proteome reflects the physiological state of the cardiovascular system and has been used to analyze plasma biomarkers for cardiovascular diseases for decades. However, current plasma biomarkers only address a limited subset of cardiovascular diseases, and there is an urgent need for biomarkers for diseases with increasing incidence like heart failure and abdominal aortic aneurysm. Recent advancements in technologies for analyzing the human plasma proteome are addressing the complexity and facilitating the discovery of novel biomarkers for cardiovascular diseases.
Review
Chemistry, Analytical
Ketki Bagwe, Noah Gould, Kendall R. Johnson, Alexander R. Ivanov
Summary: Tissues and cell populations exhibit high levels of heterogeneity at the cellular level, making it important to analyze smaller sample sizes to capture subtle differences between cells. Capillary electrophoresis coupled with mass spectrometry (CE-MS) is a powerful technique for proteomics and metabolomics analysis at the single-cell level, and this review focuses on its applications and recent advances in sample preparation, separation, MS acquisition, and data analysis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Laurent Gatto, Ruedi Aebersold, Juergen Cox, Vadim Demichev, Jason Derks, Edward Emmott, Alexander M. Franks, Alexander R. Ivanov, Ryan T. Kelly, Luke Khoury, Andrew Leduc, Michael J. MacCoss, Peter Nemes, David H. Perlman, Aleksandra A. Petelski, Christopher M. Rose, Erwin M. Schoof, Jennifer Van Eyk, Christophe Vanderaa, John R. R. Yates III, Nikolai Slavov
Summary: Analyzing proteins from single cells by tandem mass spectrometry (MS) has the potential to accurately quantify thousands of proteins across thousands of single cells. However, various factors affecting experimental design, sample preparation, data acquisition and analysis may undermine the accuracy and reproducibility of the results. Best practices, quality controls, and data-reporting recommendations are proposed to enhance the reliability of quantitative workflows for single-cell proteomics.
Article
Biochemistry & Molecular Biology
Carsten Schmelter, Kristian Nzogang Fomo, Alina Brueck, Natarajan Perumal, Sascha D. D. Markowitsch, Gokul Govind, Thomas Speck, Norbert Pfeiffer, Franz H. H. Grus
Summary: Glaucoma is a complex optic neuropathy characterized by the loss of retinal ganglion cells (RGCs) and their axons, leading to visual function decline. The exact molecular mechanism of glaucoma remains unclear, and there is an urgent need for therapeutic strategies targeting the neurodegenerative component of this disease. The synthetic CDR1 peptide has shown beneficial effects on RGC viability in a concentration-dependent manner and has been identified as a potential biomarker candidate in glaucoma patients. It specifically interacts with retinal acidic leucine-rich nuclear phosphoprotein 32A (ANP32A) and affects the expression of various proteins related to acetyl-CoA biosynthesis and cytoskeleton regulation. The peptide's interaction with ANP32A's N-terminal leucine-rich repeat (LRR) domain suggests its translational potential for glaucoma treatment.
Article
Environmental Sciences
Benjamin Trotter, Magdalena Wilde, Julian Brehm, Evdokia Dafni, Arlinda Aliu, Georg J. Arnold, Thomas Froehlich, Christian Laforsch
Summary: Research on the effects of microplastics on freshwater organisms, particularly Daphnia, has shown that chronic exposure to polystyrene microplastics can lead to decreased body length and offspring numbers in Daphnia magna. Proteomic analysis revealed changes in protein abundance, including alterations in enzymes and important physiological pathways. This study highlights the potential impact of long-term exposure to microplastics on the fitness of aquatic organisms.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
