Article
Polymer Science
Steffen A. A. Busche, Stefan Peplau, Luc Zuhse, Desiree Steimer, Dennis D. D. August, Hans G. G. Boerner
Summary: The selective ligation of stable norbornene (Nb) with an asymmetric tetrazine (Tz) is used to connect polymer segments and produce block copolymers. The Tz/Nb inverse electron-demand Diels-Alder reaction (IEDDA) is compared with the azide (Az)-norbornene Huisgen 1,3-cycloaddition (CA) to ligate end-functionalized polymers. The Tz/Nb IEDDA shows better conversion and cleaner reactions compared to the Az/Nb CA, making it a robust and potentially scalable method for ligation.
Review
Biochemistry & Molecular Biology
Gaoxiang Zhao, Zhutie Li, Renshuai Zhang, Liman Zhou, Haibo Zhao, Hongfei Jiang
Summary: Bioorthogonal chemistry represents highly efficient and biocompatible reactions that proceed selectively and rapidly in biological situations. Tetrazine bioorthogonal reactions play an important role in in vivo imaging applications, allowing for the easy tracking of real signals through controlled fluorescence or radiation off-on mechanism.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Review
Chemistry, Multidisciplinary
Renshuai Zhang, Jiake Gao, Gaoxiang Zhao, Liman Zhou, Fandong Kong, Tao Jiang, Hongfei Jiang
Summary: Bioorthogonal chemistry allows manipulation of bioactive molecules in living systems with highly selective and biocompatible reactions. Integration of bioorthogonal chemistry with nanotechnologies broadens the biological applications of nanomaterials, with tetrazine bioorthogonal chemistry playing a crucial role due to its rapid kinetics and high selectivity. This advancement has led to developments in various areas of biomedicine.
Article
Optics
J. E. Castro, T. J. Steiner, L. Thiel, A. Dinkelacker, C. McDonald, P. Pintus, L. Chang, J. E. Bowers, G. Moody
Summary: Aluminum gallium arsenide-on-insulator (AlGaAsOI) is a promising platform for integrated quantum photonics, with large optical nonlinearities, wide tunable bandgap, low propagation loss, and a large thermo-optic coefficient. We have demonstrated edge couplers, splitters, interferometers, and waveguide crossings in AlGaAsOI with performance comparable to or exceeding other quantum photonic platforms.
Article
Chemistry, Multidisciplinary
Ritapa Chaudhuri, Thumpati Prasanth, Jyotirmayee Dash
Summary: This study demonstrates for the first time that noncanonical DNA can be used to direct the synthesis of gene modulators through macrocyclization-like reactions. The planar G-quartets present in DNA G-quadruplexes provide a reaction platform for the synthesis of peptidomimetic macrocycles. By utilizing the innate fluorescence of the formed macrocycle, its cellular localization and formation process can be monitored. This research opens up new possibilities for targeted therapeutic applications.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Review
Chemistry, Multidisciplinary
Rene Roy, Leila Mousavifar
Summary: This review summarizes the recent development in utilizing carriers with increasing simplicity and versatile chemical ligation processes to produce synthetic vaccine candidates against tumor-associated carbohydrate antigens (TACAs). It covers the structures, functions, occurrence, and biosynthesis of TACAs, and highlights the versatile alkenyl glycosides as starting materials for glycoconjugate syntheses. The review also discusses various scaffolds and carriers used to enhance and simplify glycovaccine formulations, emphasizing the importance of size, shape, densities, and carriers in achieving effective immune responses.
CHEMICAL SOCIETY REVIEWS
(2023)
Review
Biotechnology & Applied Microbiology
Chunxiao Yan, Wei Yu, Lun Yao, Xiaoyu Guo, Yongjin J. Zhou, Jiaoqi Gao
Summary: This review summarizes the characterization and engineering of promoters in methylotrophic yeasts. It discusses the applications of constitutive and inducible promoters controlled by methanol or other inducers/repressors in protein and chemical production. It also highlights efforts in promoter engineering, such as site-directed mutagenesis, hybrid promoter, and transcription factor regulation, to expand the promoter toolbox.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Sergio Gonzalez-Granda, Jesus Albarran-Velo, Ivan Lavandera, Vicente Gotor-Fernandez
Summary: The combination of metal-, photo-, enzyme-, and/or organocatalysis is a versatile approach in synthetic chemistry, particularly for the creation of chiral centers. This review focuses on the recent developments in the simultaneous use of enzymes and transition metal catalysts, as well as the integration of multiple bioprocesses and metal-catalyzed transformations in linear cascades. Challenges such as catalyst compatibility, reaction media, and cross-reactivities are addressed by strategies such as catalyst coimmobilization, compartmentalization, and flow chemistry. The review also highlights the application of bionanohybrid materials, where enzymes and metals are coimmobilized on suitable supports.
Article
Chemistry, Applied
Costanza Leonardi, Arianna Brandolese, Lorenzo Preti, Olga Bortolini, Eleonora Polo, Paolo Dambruoso, Daniele Ragno, Graziano Di Carmine, Alessandro Massi
Summary: This study presents a strategy for immobilizing chiral 2,3-bisaminocyclopropenium salt onto polystyrene and silica supports, and a procedure for converting it into cyclopropenimine superbase catalysts. The silica-supported cyclopropenimine showed superior stability and comparable efficiency in batch tests, and could be used as packing material for fixed-bed reactors. Continuous-flow experiments demonstrated satisfactory long-term stability and unaltered conversion efficiency and enantioselectivity.
ADVANCED SYNTHESIS & CATALYSIS
(2021)
Article
Engineering, Chemical
Ines Nulens, Scout Caspers, Rhea Verbeke, Alexey Kubarev, Alexander H. McMillan, Ivo F. J. Vankelecom
Summary: A new microfluidic design and operational protocol for in situ characterization of interfacial polymerization (IP) is developed in this study. The optimized parylene-coated PDMS-glass chip with a specific channel layout and dimensions is proven to be most compatible with IP and performance testing.
JOURNAL OF MEMBRANE SCIENCE
(2023)
Article
Ecology
Franziska Komossa, Flurina M. Wartmann, Peter H. Verburg
Summary: The study analyzed how different data gathering methods can support local recreation planning and found that local planners have limited knowledge of and access to scientific methods. Some potential methods, such as free listing or participatory mapping, are not yet applied for local planning. The potential use of social media data is appealing, but there is currently a lack of necessary skills for collecting and analyzing data.
LANDSCAPE AND URBAN PLANNING
(2021)
Article
Biochemistry & Molecular Biology
Rita Del Giudice, Nicolo Paracini, Tomas Laursen, Clement Blanchet, Felix Roosen-Runge, Marite Cardenas
Summary: This study expands the toolkit of lipid and detergent combinations that allow the formation of stable bicelles. Systematic sample characterization using various techniques provides a set of conditions under which bicelles can be successfully formed.
Article
Chemistry, Multidisciplinary
Qianqian Qi, Yutong Zhang, Wei Xiong, Xingyu Liu, Shuangyu Cui, Xiaofang Ye, Kaisong Zhang, Tian Tian, Xiang Zhou
Summary: In this study, the norbornene-tetrazine ligation chemistry is used to develop clickable RNA switches in biological contexts, and its potential applications are explored. The application of RNA-based norbornene-tetrazine ligation chemistry in controlling CRISPR systems and manipulating gene editing in human cells is successfully demonstrated.
Review
Immunology
David Y. Oh, Lawrence Fong
Summary: Cytotoxic CD4(+) T cells play important roles in both cancer and non-cancer environments, where they can directly kill cancer cells. They are also found in situations like infection and autoimmunity. The cytolytic mechanisms of cytotoxic CD4(+) T cells vary across different disease states.
Review
Biochemistry & Molecular Biology
Hans-Jurgen Federsel, Thomas S. Moody, Steve J. C. Taylor
Summary: Enzyme immobilization technology is important for improving enzyme performance and reducing production costs, and is widely used in synthesis. Recent promising advancements in enzyme immobilization include the use of new support technologies such as polysaccharides, magnetic particles, and DNA.
