期刊
CURRENT OPINION IN CHEMICAL BIOLOGY
卷 15, 期 2, 页码 342-346出版社
CURRENT BIOLOGY LTD
DOI: 10.1016/j.cbpa.2011.01.007
关键词
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资金
- National Institutes of Health [GM084266]
- CBI [T32GM008523]
In living systems, iron is found in many different structures, including Fe/S clusters, hemes and nonheme centers, and magnetically interacting aggregates. Understanding Fe metabolism and trafficking will require biophysical spectroscopic tools that can evaluate the types of Fe centers within entire cells and isolated organelles. Mossbauer spectroscopy will play an important role in such analyses, as it has perhaps the best combination of resolution, sensitivity, coverage, and quantifying abilities. Other spectroscopic techniques, with particular strengths, will be used in combination with Mossbauer, and results will be integrated to assess the 'ironome' of such complex samples. This integrative biophysical approach is illustrated by a discussion of iron trafficking in yeast cells.
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