Article
Spectroscopy
Lingjie Duan, Yu Zhao, Xiao Zhang, Xixi Cui, Qingtian Meng, Changzhe Zhang
Summary: Selective detection of 8-oxoG in DNA is important for genomic research and understanding its formation mechanism. Researchers developed quasi-intrinsic optical probes based on natural adenine, which showed bright fluorescence and high sensitivity to the presence of 8-oxoG.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Physics, Applied
A. Kuzin, V. Chernyshev, V. Kovalyuk, P. An, A. Golikov, S. Svyatodukh, S. Perevoschikov, I. Florya, A. Schulga, S. Deyev, G. Goltsman, D. Gorin
Summary: The search for disease biomarkers and detection techniques is crucial in modern healthcare. Extracellular vesicles (EVs) are linked to various illnesses and controlling their amount may be a promising therapeutic strategy. Researchers have developed a biosensor using photonic integrated circuits and microfluidics for real-time quantification of specific EVs.
APPLIED PHYSICS LETTERS
(2023)
Article
Biophysics
Fatemeh Shahbazi, Masoud Jabbari, Mohammad Nasr Esfahani, Amir Keshmiri
Summary: A new competitive numerical platform for designing microfluidic-integrated biosensors has been developed, allowing for modifications of various properties to design specific biosensors for point-of-care applications. Validation against experimental data and analysis of design parameters have highlighted the importance of optimization in enhancing biosensor performance.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Cell Biology
Mingyue Zheng, Guoxiang Jin, Zhongjun Zhou
Summary: Lamins, ancient intermediate filament proteins, are functionally regulated by post-translational modifications, contributing to various biological functions. Deregulation of lamins is associated with abnormal nuclear morphology and chromatin disorganization, leading to diseases. Understanding these modifications provides new insights into the molecular mechanisms underlying diseases and potential therapeutic strategies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Analytical
Yi-Fan Tian, Yu-Peng Zhang, Qiu-Mei Wu, Dai-Wen Pang, Shu-Lin Liu, Zhi-Gang Wang
Summary: Translation is a critical cellular activity regulated by viruses, and studying its kinetics and subcellular localization requires real-time visualization techniques. This study presents a new method using the CRISPR/dCas13 system and specific complementary guide RNAs to dynamically image translation without altering the coding regions. This tool provides promising opportunities for further investigating translation mechanisms.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yi-Fan Tian, Yu-Peng Zhang, Qiu-Mei Wu, Dai-Wen Pang, Shu-Lin Liu, Zhi-Gang Wang
Summary: This article introduces a method for real-time dynamic translation imaging using the CRISPR/dCas13 system. The method labels the unmodified coding regions of target mRNA, enabling real-time visualization of the translation process, and is promising for further investigations into translation mechanisms.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Dimitrios Tsikas
Summary: Lysine residues in proteins undergo various chemical modifications, including carbonylation by glyoxal (GO) and methylglyoxal (MGO). Malondialdehyde (MDA) is another carbonyl species formed enzymatically and nonenzymatically. These carbonyl species can occur in free forms or be adducted to proteins, particularly lysine residues. MDA is commonly used as a biomarker of lipid peroxidation, with plasma and serum being the most frequently analyzed samples. However, preanalytical factors, such as artificial MDA formation in lipid-rich samples, can greatly affect MDA concentrations.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Dan Wu, Tao Liu
Summary: Understanding post-translational modifications is crucial for manipulating physiological processes in eukaryotes. Genetic code expansion technology has been used to investigate the roles of these modifications, and can be combined with synthetic biology to create genetically modified organisms. This article discusses the applications, limitations, and future perspectives of genetic code expansion technology for studying post-translational modifications, as well as the implications for genetically modified organisms.
Article
Biochemistry & Molecular Biology
Sharon L. Campbell, Mark R. Philips
Summary: Mutations in RAS genes are the most common driving force behind cancer development. RAS proteins, acting as binary molecular switches, control cellular growth through a complex signaling pathway. In addition to nucleotide-binding properties, RAS proteins are also regulated by numerous post-translational modifications, which are currently a high priority for drug discovery research in the field of RAS biology.
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2021)
Article
Microbiology
Laura Sattler, Peter L. Graumann
Summary: mRNA molecules exhibit both free diffusion and constrained motion within cells, with no significant difference in diffusion constants between differently sized molecules. Despite their large size, mRNA molecules are able to reach all positions within cells in seconds.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Robotics
Pengkun Zhou, Yuzhen Liu, Ziyang Meng
Summary: The paper introduces an online real-time stereo system, PointSLOT, that can simultaneously perform SLAM and dynamic object tracking. Unlike previous methods, this system identifies moving and static objects by computing object motion probabilities, and utilizes features from static objects to improve camera pose estimation. Through camera-centric parameterization and object-based bundle adjustment, it efficiently computes trajectories and dynamic landmarks of multi-keyframe objects. Evaluations on KITTI datasets and real-world experiments demonstrate that this system achieves comparable results to state-of-the-art solutions on both camera motion estimation and dynamic object tracking. The open-source code is available at https://github.com/pkzhou/PointSLOT.
IEEE ROBOTICS AND AUTOMATION LETTERS
(2023)
Article
Agriculture, Multidisciplinary
Qibin Wu, Zhenxiang Li, Jingtao Yang, Fu Xu, Xueqin Fu, Liping Xu, Chuihuai You, Dongjiao Wang, Yachun Su, Youxiong Que
Summary: This study reports the first comprehensive analysis of protein lysine acetylation, 2-hydroxyisobutyrylation, and lysine lactylation in sugarcane. These post-translational modifications were found to be involved in energy metabolism and stress response. The results provide new insights into the molecular mechanisms of protein PTMs in sugarcane.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Oncology
Ana Gonzalez-Garcia, Antonio Garrido, Ana C. Carrera
Summary: Genetic alterations in the PI3-kinase/PTEN pathway are common in cancer cells. Current efforts to treat PTEN-dependent tumors mainly focus on PI3-kinase inhibition, but modulating PTEN post-translational modifications could provide alternative therapeutic strategies.
Review
Oncology
Na Liu, Rui Ling, Xiang Tang, Yunpeng Yu, Yuepeng Zhou, Deyu Chen
Summary: BRD4, a member of the BET family, is considered a major driver of cancer cell growth and new target for cancer therapy. However, resistance to BET inhibitors and the complexity of BRD4 expression mechanism and action pathway pose challenges. The role of BRD4 in tumors is closely related to posttranslational modifications, including ubiquitination and phosphorylation, which contribute to BETi resistance and biological functions of BRD4.
FRONTIERS IN ONCOLOGY
(2022)
Article
Cell Biology
Reeja Maria Cherian, Chandra Prajapati, Kirsi Penttinen, Martta Hakli, Janne T. Koivisto, Mari Pekkanen-Mattila, Katriina Aalto-Setala
Summary: Human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) have great potential in the cardiovascular field. By using CRISPR-Cas9 genome editing technology, researchers have generated a cardiac-specific hiPSC reporter line that differentiates into fluorescent CMs, allowing for non-invasive tracking and functional phenotyping. This versatile imaging tool has significant potential in disease modeling, drug screening, and cytocompatibility analysis of hiPSC-CMs cultured in biomaterial scaffolds.
CELL BIOLOGY AND TOXICOLOGY
(2023)
