期刊
JOURNAL OF NEUROSCIENCE
卷 35, 期 9, 页码 3725-3733出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.4546-14.2015
关键词
astrocyte; BMP; integrin; neural stem cell; spinal cord injury
资金
- Craig H. Neilsen Foundation [190465]
- National Institutes of Health [R01 NS20778, R01 EB003806]
Astrogliosis after spinal cord injury (SCI) is a major impediment to functional recovery. More than half of new astrocytes generated after SCI are derived from ependymal zone stem cells (EZCs). We demonstrate that expression of beta 1-integrin increases in EZCs following SCI in mice. Conditional knock-out of beta 1-integrin increases GFAP expression and astrocytic differentiation by cultured EZCs without altering oligodendroglial or neuronal differentiation. Ablation of beta 1-integrin from EZCs in vivo reduced the number of EZC progeny that continued to express stem cell markers after SCI, increased the proportion of EZCprogeny that differentiated intoGFAP + astrocytes, and diminished functional recovery. Loss of beta 1-integrin increased SMAD1/5/8 and p38 signaling, suggesting activation of BMP signaling. Coimmunoprecipitation studies demonstrated that beta 1-integrin directly interacts with the bone morphogenetic protein receptor sub-units BMPR1a and BMPR1b. Ablation of beta 1-integrin reduced overall levels of BMP receptors but significantly increased partitioning of BMPR1b into lipid rafts with increased SMAD1/5/8 and p38 signaling. Thus beta 1-integrin expression by EZCs reduces movement of BMPR1b into lipid rafts, thereby limiting the known deleterious effects of BMPR1b signaling on glial scar formation after SCI.
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