Article
Biophysics
Mengyue Pei, Rong Xu, Chuangnian Zhang, Xiaoli Wang, Chen Li, Yunzhang Hu
Summary: This study designed mannose-functionalized antigen nanoparticles with endosome escape activity for targeted DCs and enhanced MHC-I antigen presentation. Results showed that these nanoparticles significantly increased antigen uptake by DCs, facilitated cytosolic antigen release, and promoted cytokine production and DCs maturation. Moreover, the nanoparticles treated DCs exhibited enhanced cross-presentation to T cells in vitro.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Review
Immunology
Laura Santambrogio
Summary: Advances in the analysis of the MHC class II ligandome have increased our understanding of the factors that regulate the range and selection of presented peptides, and the landscape is highly sensitive to changes in protein composition.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Binkai Chi, Muhammet M. Oeztuerk, Christina L. Paraggio, Claudia E. Leonard, Maria E. Sanita, Mahtab Dastpak, Jeremy D. O'Connell, Jordan A. Coady, Jiuchun Zhang, Steven P. Gygi, Rodrigo Lopez-Gonzalez, Shanye Yin, Robin Reed
Summary: Mutations in RNA/DNA-binding proteins can cause ALS, but the exact disease mechanisms are still unclear. This study found that a group of ALS-associated proteins can affect the expression of genes involved in the MHC II antigen presentation pathway. Additionally, hematopoietic progenitor cells with mutations also exhibit disrupted MHC II expression. These findings suggest that the loss of the MHC II pathway may result in the immune system's failure to protect motor neurons from ALS-related damage.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Agnes Ulfig, Verian Bader, Marharyta Varatnitskaya, Natalie Lupilov, Konstanze F. Winklhofer, Lars Leichert
Summary: Studies have shown that N-chlorinated HSA can significantly impair the ability of macrophages to present antigens to T cells via MHC class II proteins at multiple stages, potentially preventing antigen processing by immune cells at the initial stage of infection and leading to chronic infection and inflammation.
Article
Pharmacology & Pharmacy
Carley Tasker, Jenny Patel, Vibha Jawa, Jad Maamary
Summary: A novel cell-based assay has been proposed for investigating the endosomal processing and MHC class II presentation capabilities of antigens, utilizing competition between epitopes for MHC class II binding and labeled soluble T cell receptors as detectors for epitope presentation.
Article
Immunology
Julia Diegelmann, Stephan Brand
Summary: Antigen presentation through MHC I and MHC II receptors is crucial for T cell-mediated adaptive immunity, and non-professional antigen presenting cells such as intestinal epithelial cells (IEC) may participate in this process by expressing MHC II. This study identifies IL-27 as a regulator of MHCI and MHCII receptor expression in IEC, highlighting the importance of IEC as non-professional APCs.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Immunology
Karthik Dhatchinamoorthy, Jeff D. Colbert, Kenneth L. Rock
Summary: Loss of MHC I antigen presentation is common in many cancers, which may impair immune responses and affect the efficacy of immunotherapy. Studies have discussed underlying mechanisms through which some cancers evade immune killing by shutting down the MHC I pathway, and proposed potential strategies to overcome this limitation.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Cell Biology
Irina A. Ishina, Maria Y. Zakharova, Inna N. Kurbatskaia, Azad E. Mamedov, Alexey A. A. Belogurov Jr, Alexander G. G. Gabibov
Summary: Antigen presentation by MHC-II is crucial for immune response and self-antigen tolerance. Certain MHC-II alleles are associated with autoimmune diseases. The process of MHC-II-mediated presentation plays a significant role in understanding the mechanisms of autoimmune diseases and the protective effect of certain MHC-II alleles.
Review
Medicine, Research & Experimental
Tingting Zhang, Adila Aipire, Yijie Li, Changying Guo, Jinyao Li
Summary: This review summarizes the mechanisms of cross-presentation (XPT) and its application in tumor immunotherapy. Dendritic cells enhance their antigen presentation capacity through receptor-mediated internalization of exogenous antigens, endosome escape, engagement of other XPT-related proteins, and the use of adjuvants, thereby improving the outcomes of DC-based therapy.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Oncology
Lei Zhan, Junhui Zhang, Jing Zhang, Xiaojing Liu, Suding Zhu, Yuchuan Shi, Yu He, Wenyan Wang, Yijing Wei, Zhenhai Tang, Guo Chen, Bing Wei, Yunxia Cao
Summary: In this study, the researchers found that upregulated autophagy and decreased expression of MHC-I and NLRC5 were associated with endometrial cancer (EC). Inhibition of autophagy was found to suppress MHC-I gene expression. They also discovered that LC3 interacted with NLRC5 to inhibit the NLRC5-mediated MHC-I antigen presentation pathway. These findings suggest that inhibiting LC3 and promoting NLRC5 may be a promising immunotherapy strategy for EC management.
Review
Biochemistry & Molecular Biology
M. L. M. Jongsma, J. Neefjes, R. M. Spaapen
Summary: MHC-I molecules present a blueprint of the intracellular proteome to T cells for immune response, but pathogens and tumor cells can downmodulate MHC-I mediated antigen presentation to evade immune surveillance. While the fundamental rules of antigen presentation are well understood, new modules of regulation in this system continue to be uncovered.
MOLECULAR IMMUNOLOGY
(2021)
Article
Biochemical Research Methods
Laura C. Demmers, Wei Wu, Albert J. R. Heck
Summary: HLA molecules play critical roles in the adaptive immune system by presenting small peptides to signal cell health status to the immune system. This study investigated the adaptive response of a B lymphoblastic cell line to high temperature treatment, revealing potential preparations for immune-like responses in the absence of invading pathogenic peptides. The findings suggest intriguing temperature-sensitive adaptations in this particular B cell line.
MOLECULAR & CELLULAR PROTEOMICS
(2021)
Article
Multidisciplinary Sciences
Haiyin Liu, Kayla R. Wilson, Ashley M. Firth, Christophe Macri, Patrick Schriek, Annabelle B. Blum, Javiera Villar, Samuel Wormald, Mitch Shambrook, Bangyan Xu, Hui Jing Lim, Hamish E. G. McWilliam, Andrew F. Hill, Laura E. Edgington-Mitchell, Irina Caminschi, Mireille H. Lahoud, Elodie Segura, Marco J. Herold, Jose A. Villadangos, Justine D. Mintern
Summary: This study reveals the critical role of ubiquitin-like protein 3 (UBL3) in the trafficking process controlled by MARCH1. UBL3 has wide-ranging immunological consequences and is essential for immune responses.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Nyerhovwo Obarorakpor, Deep Patel, Reni Boyarov, Nansalmaa Amarsaikhan, Joseph Ray Cepeda, Doreen Eastes, Sylvia Robertson, Travis Johnson, Kai Yang, Qizhi Tang, Li Zhang
Summary: In spontaneous type 1 diabetes (T1D) non-obese diabetic (NOD) mice, the insulin B chain peptide 9-23 (B:9-23) can bind to the MHC class II molecule (IA(g7)) in register 3 (R3), creating a bimolecular IA(g7)/InsulinB:9-23 register 3 conformational epitope (InsB:R3). InsB:R3-specific chimeric antigen receptor (CAR) can guide CAR-expressing CD8 T cells to migrate to the islets and pancreatic lymph nodes. Regulatory T cells (Tregs) specific for an islet antigen can suppress autoimmune reactivity in islets and protect against T1D.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jieru Deng, Chunni Lu, Chuanxin Liu, Sara Oveissi, W. Douglas Fairlie, Erinna F. Lee, Pamuk Bilsel, Hamsa Puthalakath, Weisan Chen
Summary: The study demonstrates that IAV infection induces endogenous presentation of a viral epitope by MHC-II to CD4(+) T cells, dependent on de novo protein synthesis and the ER-Golgi network. Some antigenic peptides are of cytosolic origin in this process. Additionally, autophagy inhibitors and deletion of autophagy-related genes block most of the MHC-II-restricted endogenous IAV antigen presentation.
Article
Cell Biology
Frederik J. Verweij, Celine Revenu, Guillaume Arras, Florent Dingli, Damarys Loew, D. Michiel Pegtel, Gautier Follain, Guillaume Allio, Jacky G. Goetz, Pascale Zimmermann, Philippe Herbomel, Filippo Del Bene, Graca Raposo, Guillaume van Niel
DEVELOPMENTAL CELL
(2019)
Article
Multidisciplinary Sciences
D. A. C. Heesterbeek, N. Martin, A. Velthuizen, M. Duijst, M. Ruyken, R. Wubbolts, S. H. M. Rooijakkers, B. W. Bardoel
SCIENTIFIC REPORTS
(2019)
Article
Microbiology
Xinyue Li, Nancy M. C. Bleumink-Pluym, Yvette M. C. A. Luijkx, Richard W. Wubbolts, Jos P. M. van Putten, Karin Strijbis
Correction
Multidisciplinary Sciences
D. A. C. Heesterbeek, N. I. Martin, A. Velthuizen, M. Duijst, M. Ruyken, R. Wubbolts, S. H. M. Rooijakkers, B. W. Bardoel
SCIENTIFIC REPORTS
(2019)
Article
Biochemical Research Methods
Maarten P. Bebelman, Philippe Bun, Stephan Huveneers, Guillaume van Niel, D. Michiel Pegtel, Frederik J. Verweij
Article
Biology
Olivier Thouvenin, Ludovic Keiser, Yasmine Cantaut-Belarif, Martin Carbo-Tano, Frederik Verweij, Nathalie Jurisch-Yaksi, Pierre-Luc Bardet, Guillaume van Niel, Francois Gallaire, Claire Wyart
Review
Biochemistry & Molecular Biology
Anais Becot, Charlotte Volgers, Guillaume van Niel
Review
Chemistry, Analytical
Marco Morani, Thanh Duc Mai, Zuzana Krupova, Guillaume van Niel, Pierre Defrenaix, Myriam Taverna
Summary: Extracellular vesicles (EVs) are a family of cell-derived membrane vesicles present in almost all body fluids, playing important roles in various patho-physiological conditions. Knowledge about the features of individual EVs is still in its infancy, but electrokinetically driven methods provide new trends for isolation and characterization of EVs.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Valentina Biagini, Federica Busi, Viviana Anelli, Emanuela Kerschbamer, Marta Baghini, Elena Gurrieri, Michela Notarangelo, Isabella Pesce, Guillaume van Niel, Vito G. D'Agostino, Marina Mione
Summary: Extracellular vesicles (EVs) are membranous particles released by all cell types and play a role as functional carriers of bioactive molecules. Zebrafish melanoma iEVs were isolated and characterized, showing a diameter of approximately 140 nm. These iEVs contain MRP- and P-RNAs, which can induce an inflammatory response in cells of the innate immune system.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Guillaume van Niel, David R. F. Carter, Aled Clayton, Daniel W. Lambert, Graca Raposo, Pieter Vader
Summary: Extracellular vesicles (EVs) play a significant role in cell-cell communication, but there are still many questions regarding their delivery in vivo. This article highlights the progress and challenges in understanding the importance of EV-mediated communication and provides recommendations for addressing these issues.
NATURE REVIEWS MOLECULAR CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Roberta Palmulli, Enzo Bresteau, Graca Raposo, Guillaume Montagnac, Guillaume van Niel
Summary: Extracellular vesicles (EVs) are actively involved in melanoma progression by modifying the tumor microenvironment and promoting the formation of pre-metastatic niche. The interaction of tumor-derived EVs with the extracellular matrix (ECM) plays a critical role in promoting persistent tumor cell migration. However, the direct interaction between EVs and ECM components is still uncertain. In this study, we utilized electron microscopy and a pull-down assay to demonstrate that sEVs derived from melanoma cell lines can physically interact with collagen I, forming collagen fibrils coated with sEVs, highlighting the heterogeneity of sEVs' interaction with collagen.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Maria Laura Tognoli, Julia Dancourt, Emeline Bonsergent, Roberta Palmulli, Olivier G. de Jong, Guillaume Van Niel, Eric Rubinstein, Pieter Vader, Gregory Lavieu
Summary: Extracellular vesicles (EVs) play a role in intercellular communication by transferring substances from donor to acceptor cells. The process of EV content delivery within acceptor cells and the involvement of CD63 and CD9 tetraspanins in this process have been unclear. This study used different cell models to investigate the potential role of CD63 and CD9 in EV uptake and cargo delivery, and found that neither CD63 nor CD9 is required for these functions.
COMMUNICATIONS BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Vincenzo Verdi, Anais Becot, Guillaume van Niel, Frederik J. Verweij
Summary: Intercellular communication in multicellular organisms critically depends on extracellular vesicles (EVs), nano-sized lipid bilayer vesicles enriched in biomolecules. Despite numerous studies on EV dynamics in vitro, studying EV biology in vivo remains challenging. This review focuses on the state of EV imaging in vivo, with a particular emphasis on using zebrafish as a model to visualize and study endogenous EVs in real-time.
Article
Immunology
Stephanie Charrin, Roberta Palmulli, Martine Billard, Denis Clay, Claude Boucheix, Guillaume Van Niel, Eric Rubinstein
Article
Cell Biology
Marthe F. S. Lindenbergh, Richard Wubbolts, Ellen G. F. Borg, Esther M. van 'T Veld, Marianne Boes, W. Stoorvogel
JOURNAL OF EXTRACELLULAR VESICLES
(2020)