期刊
CURRENT OPINION IN BIOTECHNOLOGY
卷 23, 期 5, 页码 766-772出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.copbio.2012.05.003
关键词
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资金
- Agencia Nacional de Promocion Cientifica y Tecnologica
- National Research Council (CONICET)
- Alexander von Humboldt Foundation [1141629]
- Excellence Initiative of the German Federal and State Governments [EXC 294]
Bacterial signal-responsive regulatory circuits have been employed as platform to design and construct whole-cell bacterial biosensors for reporting toxicity. A new generation of biosensors with improved performance and a wide application range has emerged after the application of synthetic biology concepts to biosensor design. Site-directed mutagenesis, directed evolution and domain swapping were applied to upgrade signal detection or to create novel sensor modules. Rewiring of the genetic circuits allows improving the determinations and reduces the heterogeneity of the response between individual reporter cells. Moreover, the assembly of natural or engineered modules to biosensor platforms provides innovative outputs, expanding the range of application of these devises, from monitoring toxics and bioremediation to killing targeted cells.
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