Article
Cell Biology
Yonghua Zheng, Huaxia Ji, Wulin Yi, Zhanjun Chen, Xiaobiao Hu, Jie Zhou, Yang Wang, Xiao Zheng
Summary: PRMT5 is overexpressed in lung cancer cells and tissues, and its expression is induced by hypoxia. Inhibiting or silencing PRMT5 disrupts the phosphorylation of the VEGFR/Akt/eNOS angiogenic signaling pathway, NOS activity, and NO production. Additionally, inhibiting PRMT5 activity reduces HIF-1a expression and stability, resulting in the down-regulation of the VEGF/VEGFR signaling pathway. Our study provides compelling evidence of the close association between PRMT5 and angiogenesis/EMT and highlights the potential of targeting PRMT5 activity as a promising therapeutic approach for treating lung cancer with abnormal angiogenesis.
Article
Cardiac & Cardiovascular Systems
Hideki Amano, Koji Eshima, Yoshiya Ito, Masaki Nakamura, Hidero Kitasato, Fumihiro Ogawa, Kanako Hosono, Kazuya Iwabuchi, Satoshi Uematsu, Shizuo Akira, Shuh Narumiya, Masataka Majima
Summary: mPGES-1/PGE(2) induces neovascularization from ischemia via EP4 by promoting the accumulation of Tregs.
CARDIOVASCULAR RESEARCH
(2023)
Article
Cell Biology
Yanqing Liu, Shiyu Chen, Kun Cai, Dijie Zheng, Changhao Zhu, Lin Li, Feiqing Wang, Zhiwei He, Chao Yu, Chengyi Sun
Summary: This study investigated the biological effects and regulatory mechanisms of NR2F1-AS1 in pancreatic cancer. The overexpression of NR2F1-AS1 was found to be correlated with poor prognosis and aggressive behavior in pancreatic cancer. Depletion of NR2F1-AS1 inhibited cell proliferation, migration, invasion, and tumor growth and metastasis in vitro and in vivo. Mechanistically, NR2F1-AS1 positively regulated the neighboring NR2F1 gene, leading to the activation of AKT/mTOR signaling and upregulation of HIF-1 alpha. Further investigations also revealed that NR2F1-AS1 expression was transcriptionally regulated by HIF-1 alpha under hypoxic conditions.
CELL DEATH & DISEASE
(2022)
Article
Oncology
Yueyang Mou, Yaqin Hu, Guolian Xue, Wenjian Zhao, Yang Jiao, Chen Li, Haiyan Cao, Min Chao, Jianping Deng, Penggao Dai, Hongli Zhu, Liang Wang
Summary: ELK3 plays a crucial role in glioma angiogenesis by activating the HIF-1 alpha/VEGF-A signaling axis. It may serve as a prognostic marker for glioma angiogenesis and targeting ELK3 may improve the efficacy of anti-angiogenic therapies.
Article
Medicine, Research & Experimental
Jun-Song Ren, Wen Bai, Jing-Juan Ding, Hui-Min Ge, Su-Yu Wang, Xi Chen, Qin Jiang
Summary: Pathological neovascularization plays a crucial role in tumors and neovascular eye diseases. The widespread application of anti-angiogenic drugs targeting VEGF and VEGFRs is hindered by adverse reactions and drug resistance. Pan-cancer analysis and single-cell sequencing analysis are important methods to discover potential therapeutic targets related to the regulation of pathological neovascularization.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Immunology
Jingwen Xu, Yabing Zheng, Yaqing Zhao, Yujiao Zhang, Huilin Li, An Zhang, Xuehan Wang, Weizong Wang, Yinglong Hou, Jiangrong Wang
Summary: This study found that succinate levels were significantly higher in arterial serum of coronary heart disease (CHD) patients and were positively correlated with interleukin-1 beta (IL-1β). In vitro experiments demonstrated that succinate could activate the Hif-1α/IL-1β signaling pathway and promote the inflammatory response in human umbilical vein endothelial cells (HUVECs). Animal experiments showed that injection of succinate aggravated the inflammatory phenotype in the aortic sinus. Furthermore, a Sucnr1 inhibitor disrupted the succinate/IL-1β signaling in HUVECs and macrophages.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Zhiguo Niu, Jianing Fan, Fengzhe Chen, Huijie Yang, Xin Li, Ting Zhuang, Chunlei Guo, Qi Cao, Jian Zhu, Hui Wang, Qingsong Huang
Summary: Breast cancer is the most common malignancy in women globally. Abnormal expression of RBCK1 protein in breast tumors makes it a valuable diagnostic marker and drug target. Hypoxic regions in breast cancer activate the HIF1 pathway, influencing tumor recurrence and metastasis. Targeting RBCK1 could be a promising treatment strategy for ER-positive breast cancer.
CELL DEATH & DISEASE
(2022)
Article
Oncology
Wenrun Cai, Xugang Sun, Fanjie Jin, Di Xiao, Hui Li, Huizhi Sun, Yifei Wang, Yang Lu, Jing Liu, Chongbiao Huang, Xiuchao Wang, Song Gao, Hongwei Wang, Chuntao Gao, Tiansuo Zhao, Jihui Hao
Summary: CAFs in PDAC promote tumor angiogenesis through MEndoT, with their phenotypic transition being regulated by the PERK-eIF2 alpha-ERK1/2 axis. Pharmacological inhibition of PERK may limit the transition of CAFs, suggesting they could be therapeutic targets for improving PDAC prognosis.
Article
Biochemistry & Molecular Biology
Aranka Brockmueller, Sosmitha Girisa, Ajaikumar B. B. Kunnumakkara, Mehdi Shakibaei
Summary: Resveratrol can enhance the sensitivity of colorectal cancer cells to 5-FU by regulating the beta 1-integrin/HIF-1α signaling axis, leading to suppression of cancer cell growth and invasion, inhibition of inflammation and cancer stem cell production. These findings highlight the potential application of resveratrol in the treatment of colorectal cancer by overcoming chemoresistance to 5-FU.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Yiping Zhu, Fang Wu, Weiwei Gui, Nan Zhang, Erik Matro, Linghua Zhu, Daniel Turunen Eserberg, Xihua Lin
Summary: The reciprocal regulation between PVT1 and HIF-1α in pancreatic cancer forms a positive feedback loop, which could be a potential therapeutic target for the treatment of PC.
JOURNAL OF MOLECULAR CELL BIOLOGY
(2021)
Article
Plant Sciences
Jingwen Xu, Zhenhua Tian, Zhe Li, Xiaoshi Du, Yansong Cui, Jiangrong Wang, Mei Gao, Yinglong Hou
Summary: This study found that Puerarin and Tanshinone IIA (Pue-Tan) inhibit the formation of inflammatory plaques and delay the progression of atherosclerosis by targeting the succinate signaling axis. The key target of Pue-Tan and the key node of the succinate signaling axis was found to be HIF-1 alpha.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Cell & Tissue Engineering
Toshiharu Yamashita, Cat-Khanh Vuong, Nhat-Hoang Ngo, Motoo Osaka, Yuji Hiramatsu, Osamu Ohneda
Summary: Long-term glucocorticoid treatment impairs wound healing ability in adipose tissue-derived mesenchymal stem cells (sAT-MSC) due to downregulation of SDF-1. Our study found that sAT-MSC showed impaired HIF-1a and upregulated HIF-2a levels, with HIF-2a inhibition leading to compensatory overexpression of HIF-1a and SDF-1, improving wound healing ability. Additionally, HIF-2a reduction promoted wound healing effects and increased inflammatory cell recruitment through HIF-1a/SDF-1 axis.
STEM CELLS AND DEVELOPMENT
(2023)
Article
Medicine, Research & Experimental
Asli Okan, Zuleyha Doganyigit, Ece Eroglu, Enes Akyuz, Necdet Demir
Summary: The study aimed to evaluate the immunoexpression of adaptive responses to hypoxia in pancreatic and cardiac tissues of type 1 DM mice. It was found that TNF-alpha, HIF-1 alpha, and Kir6.2 immunoreactivity significantly increased in both tissues, suggesting potential roles of these factors in the mechanism of type 1 DM.
Article
Biochemistry & Molecular Biology
Rui Liang, Na Liu, Jinglin Cao, Tengli Liu, Peng Sun, Xiangheng Cai, Lanqiu Zhang, Yaojuan Liu, Jiaqi Zou, Le Wang, Xuejie Ding, Boya Zhang, Zhongyang Shen, Sei Yoshida, Jian Dou, Shusen Wang
Summary: Our study revealed that HIF-1 alpha/FOXO1 regulated autophagy benefits beta cell survival under hypoxia and autophagy dysregulation may account for beta cell mass loss in T2DM.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Article
Oncology
Anna Russignan, Giada Dal Collo, Anna Bagnato, Nicola Tamassia, Mattia Bugatti, Mirella Belleri, Luisa Lorenzi, Enrica Borsi, Riccardo Bazzoni, Michele Gottardi, Carolina Terragna, William Vermi, Arianna Giacomini, Marco Presta, Marco Antonio Cassatella, Mauro Krampera, Cristina Tecchio
Summary: Macitentan effectively targets ET-1 receptors to inhibit MM cell survival and reduce pro-angiogenic cytokine release by modulating MAPK/ERK and HIF-1 alpha pathways, suggesting its potential as an anti-proliferative and anti-angiogenic therapy in MM preclinical models.
FRONTIERS IN ONCOLOGY
(2021)
Article
Engineering, Biomedical
Guangbing Wei, Zijun Wang, Ruilin Liu, Cancan Zhou, Enmeng Li, Tianli Shen, Xingjie Wang, Yunhua Wu, Xuqi Li
Summary: In this study, hybrid polydopamine (PDA)-KGF nanoparticles (PDA-KGF NPs) combined with hyaluronate (Ha) were successfully prepared to effectively prevent the occurrence of abdominal adhesions, promote the repair of injured peritoneal mesothelial cells, and reduce collagen deposition and fibrosis, as well as inhibit inflammatory response.
ACTA BIOMATERIALIA
(2022)
Review
Chemistry, Medicinal
Dan Qi, Yunyi Liu, Juan Li, Jason H. Huang, Xiaoxiao Hu, Erxi Wu
Summary: Cancer stem cells (CSCs) are a small subpopulation of cells within a tumor that play a crucial role in various aspects of cancer development, making them attractive targets for cancer treatment. Salinomycin, identified as a potent anti-CSC agent in 2009, has broad-spectrum activities and potential for clinical application based on current understandings. Further research is needed to explore its mechanisms of action and translational potential.
MEDICINAL RESEARCH REVIEWS
(2022)
Article
Multidisciplinary Sciences
Xin Chen, Liang Sheng, Jiguang Ma, Dan Qi, Xuqi Li, Zheng Wang, Zheng Wu, Lucas Wong, Jason H. Huang, Erx Wu, Qingyon Ma, Dong Zhang
Summary: Cigarette smokers have a higher risk of pancreatic cancer, but the mechanisms of cigarette metabolites are not fully understood. This study demonstrates that NNK, a component of cigarette smoke, promotes the growth and migration of pancreatic cancer cells and the progression of chronic pancreatitis to pancreatic cancer. These findings provide potential strategies and targets for early prevention and treatment of pancreatic cancer.
Article
Engineering, Biomedical
Yunyi Liu, Cheng Peng, Hui Zhang, Juan Li, Hailong Ou, Yang Sun, Chaoqi Wen, Dan Qi, Xiaoxiao Hu, Erxi Wu, Weihong Tan
Summary: This study demonstrates that DNA aptamer S11e can accurately recognize fibrosarcoma cells and localize in the mitochondria, promoting cell apoptosis and inhibiting cell migration. Additionally, the study reveals the interaction between S11e and Diablo/SMAC protein, leading to the inhibition of fibrosarcoma cell migration and invasion.
BIOACTIVE MATERIALS
(2022)
Article
Multidisciplinary Sciences
Jing Lu, Ying Zhang, Dan Qi, Chunyan Yan, Benhao Wu, Jason H. Huang, Jianwen Yao, Erxi Wu, Guoying Zhang
Summary: Recent research has discovered that L-theanine from tea and its derivative TBrC have potential inhibitory activities against SARS-CoV-2, as well as anti-tumor effects on lung cancer cells without affecting normal lung cells. This suggests a potential protective effect of TBrC and L-theanine against pulmonary damage in patients infected with SARS-CoV-2, particularly lung cancer patients.
Review
Clinical Neurology
Dan Qi, Jing Li, C. Chad Quarles, Ekokobe Fonkem, Erxi Wu
Summary: This article summarizes the current challenges in the assessment of therapy response in patients with glioblastoma. It discusses the use of quantitative imaging, liquid biomarkers, and machine intelligence for the early prediction of therapy response and tumor progression to guide clinical decision making.
Article
Genetics & Heredity
Dan Qi, Yiqun Geng, Jacob Cardenas, Jinghua Gu, S. Stephen Yi, Jason H. H. Huang, Ekokobe Fonkem, Erxi Wu
Summary: Peripheral blood is being explored as a noninvasive alternative to tissue biopsy for developing biomarkers for glioblastoma (GBM), but the lack of a robust detection approach has hindered the identification of widely utilized blood-based biomarkers in clinical settings. This study introduces WBGR, a globin reduction technique for RNA sequencing of whole blood, and demonstrates its ability to detect GBM-associated transcriptomic changes. Through transcriptomic analysis of tumor tissues, a 10-gene panel (GBM-Dx panel) consisting of mRNA, long noncoding RNA, and microRNA was identified as a potential tool for early detection and clinical management of GBM. The integrated approach of WBGR offers comprehensive analytic capacity for blood-based marker identification.
NPJ GENOMIC MEDICINE
(2023)
Article
Biochemical Research Methods
Xingxin Pan, Zeynep H. Coban Akdemir, Ruixuan Gao, Xiaoqian Jiang, Gloria M. Sheynkman, Erxi Wu, Jason H. Huang, Nidhi Sahni, S. Stephen Yi
Summary: Alzheimer's disease (AD) is a complex neurodegenerative disease with genetic factors playing a key role in its occurrence. Previous studies on AD-associated genes have been limited by sample size and selection bias, calling for comprehensive research. To address this, we constructed a large-scale AD mutation framework and proposed deep learning models to effectively predict cognitive impairment based on genetic mutation profiles. Our study identified novel co-mutations that contribute to dementia, laying a foundation for targeted therapy and precision medicine.
BRIEFINGS IN BIOINFORMATICS
(2023)
Article
Immunology
Yuqian Liu, Shenjie Wang, Yixuan Wang, Yifei Li, Xiaoyan Zhu, Xin Lai, Xuanping Zhang, Xuqi Li, Xiao Xiao, Jiayin Wang
Summary: Tumor mutation burden (TMB) is a widely recognized biomarker for predicting the efficacy of immunotherapy, but its use remains controversial. This study examines the causes of this controversy and identifies the conflict between incomplete biostatistics rules and the variety of clinical samples as a critical issue. The challenges of mutation detection in clinical practice are illustrated, and potential strategies for overcoming these conflict issues are discussed to enable the application of TMB in real clinical settings.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Mengke Zhu, Chao Wei, Haijiang Wang, Shangning Han, Lindi Cai, Xiaowen Li, Xinhua Liao, Xiangming Che, Xuqi Li, Lin Fan, Guanglin Qiu
Summary: This study investigates the effects and underlying mechanism of SIRT1 on autophagy and malignant biological behavior of gastric cancer cells under conditions of glucose deprivation. Results show that SIRT1 regulates the activity of FoxO1 and upregulates the expression of Rab7 through deacetylation, thereby affecting autophagy in gastric cancer cells. Decreasing the expression of SIRT1, FoxO1 or Rab7 significantly inhibits autophagy levels, increases sensitivity to glucose deprivation, and enhances the inhibition of proliferation, migration, and invasion, as well as apoptosis in gastric cancer cells induced by glucose deprivation.
FRONTIERS IN ONCOLOGY
(2023)
Article
Surgery
Xiaoqiang Shi, Hainan Guo, Chao Zhu, Guanglin Qiu, Ting Liang, Jie Lian, Yanfei Ma, Shufeng Wang, Xuqi Li
Summary: This study evaluated the feasibility and application value of mixed reality technology in primary retroperitoneal tumor surgery. The results showed that MR could assist PRT resection and had the potential to improve the rate of complete tumor resection.
INTERNATIONAL JOURNAL OF MEDICAL ROBOTICS AND COMPUTER ASSISTED SURGERY
(2023)
Article
Oncology
Longwen Xu, Mengjie Liu, Jie Lian, Enmeng Li, Chang Dongmin, Xuqi Li, Wenjuan Wang
Summary: We developed a Treg-related classifier and generated a prognostic nomogram that predicts recurrence-free survival in stage I-III colon cancer. This classifier can identify high-risk patients for more personalized and effective therapy. The performance of the classifier was validated using multiple datasets and compared with other signatures.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Surgery
Zijun Wang, Enmeng Li, Cancan Zhou, Bolun Qu, Tianli Shen, Jie Lian, Gan Li, Yiwei Ren, Yunhua Wu, Qinhong Xu, Guangbing Wei, Xuqi Li
Summary: In this study, an optimized mouse model was used to observe the progression of abdominal adhesions. The inflammatory response was found to be activated early in the process, peaking on day 3 and then gradually decreasing until stabilization on day 7. Collagen and fibronectin formation also occurred early and increased until remaining stable on day 7. Live mice observation and RNA sequencing revealed potential key targets involved in abdominal adhesion formation at different time points. The establishment of a standard process for describing the evolution of abdominal adhesions will facilitate future adhesion-related studies.
JOURNAL OF INVESTIGATIVE SURGERY
(2023)
Article
Biochemistry & Molecular Biology
Xingxin Pan, Jun Yun, Zeynep H. Coban Akdemir, Xiaoqian Jiang, Erxi Wu, Jason H. Huang, Nidhi Sahni, S. Stephen Yi
Summary: Discovering effective therapies for neurological and developmental disorders is difficult due to their complex and interactive mechanisms. Existing drugs for Alzheimer's disease are limited, especially for impacting cell death causes. This study developed a deep learning-based prediction framework to identify potential repurposed drug therapies for AD, which can also be applied to other diseases. The framework includes building a drug-target pair network, incorporating various features, and developing an AI-based DrugNet with robust predictive performance.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Tianli Shen, Yunhua Wu, Xingjie Wang, Zijun Wang, Enmeng Li, Cancan Zhou, Chenyang Yue, Zhengdong Jiang, Guangbing Wei, Jie Lian, Qinhong Xu, Xuqi Li
Summary: A compound called honokiol, found in magnolia tree bark, has been found to significantly reduce the formation of internal scar tissue after abdominal surgery in mice. This could potentially prevent complications such as infertility or bowel obstructions caused by peritoneal adhesions. The mechanism behind this reduction in scar tissue formation is believed to be related to the activation of a stress-response protein called SIRT3. These findings suggest a new therapeutic strategy for preventing post-surgical peritoneal adhesions.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2022)
