Article
Microbiology
Shuo Yang, Lan Ma, Xiaoqing Xu, Qing Peng, Huiying Zhong, Yuxin Gong, Linbo Shi, Mengxin He, Bo Shi, Yu Qiao
Summary: Rhamnolipid (RL) was found to inhibit biofilm formation of Escherichia coli O157:H7, but the mechanism is still unclear. Comparative physiological and transcriptomic analyses were conducted to elucidate the potential mechanism by which RL may inhibit biofilm formation in E. coli O157:H7. The results showed that RL treatment significantly reduced outer membrane hydrophobicity, affected cell membrane integrity and permeability by increasing the release of lipopolysaccharide (LPS), and led to differential expression of genes related to biosynthesis, outer membrane protein synthesis, flagellar assembly, and pathogenicity. Overall, RL treatment inhibits biofilm formation in E. coli O157:H7 by modifying outer membrane properties and gene expression levels.
Article
Food Science & Technology
Xiaohua He, Heer Ding, Zenghui Gao, Xiaofeng Zhang, Rong Wu, Ke Li
Summary: The adaptability of Escherichia coli O157:H7 NCTC 12900 during noodle processing was investigated. The study found that bacterial surface morphology, count, and motility were impaired in the squeezing phase, while biofilm formation abilities increased across all processing phases. RT-qPCR analysis revealed that csrA, GcvB, McaS, and OxyS were the most relevant genes and sRNAs for biofilm formation and motility. Overexpression of these genes and sRNAs inhibited bacterial motility and biofilm formation to varying degrees during noodle processing.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Acoustics
Jiao Li, Donghong Liu, Tian Ding
Summary: The study investigated the gene regulation at the transcription level in Escherichia coli O157:H7 after ultrasonic stimulation, revealing significant differential expressions of 1217 genes, including up-regulated and down-regulated genes related to crucial biomolecular processes. The most enriched pathways were analyzed in carbohydrate metabolism, energy metabolism, membrane transport, signal transduction, transcription, and translation categories. Additionally, the study found that the citrate cycle genes were down-regulated, affecting carbohydrate decomposition and energy production under ultrasonic stress.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Microbiology
Mehdi Zarei, Saeid Rahimi, Per Erik Joakim Saris, Amin Yousefvand
Summary: In order to prevent biofilm formation in the dairy industry, it is important to understand the interaction between different species during biofilm formation. The study found that P. fluorescens strains are the most important biofilm-formers on the surface of dairy processing equipment, and they can attract and/or shelter other spoilage or pathogenic bacteria. The ability of the P. fluorescens strains to form dual-species biofilms with different pathogens varied depending on the inoculation scenario.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Juliana Gonzalez, Jimena Soledad Cadona, Claudio Marcelo Zotta, Silvina Lavayen, Roberto Vidal, Nora Lia Padola, Andrea Mariel Sanso, Ana Victoria Bustamante
Summary: This study aimed to compare the genetic diversity of VTEC O157:H7 strains isolated from human disease cases in Argentina and Chile. The results showed that these strains mainly belonged to lineage I/II, associated with hypervirulent strains, and were categorized into the phylogroup E. Additionally, genetic diversity was observed among Argentinean and Chilean strains, particularly in relation to putative virulence determinants and nle profiles.
MICROBES AND INFECTION
(2022)
Article
Food Science & Technology
Haiying Cui, Xiaojing Yang, Changzhu Li, Yong Ye, Xiaochen Chen, Lin Lin
Summary: A strategy to simultaneously remove Escherichia coli O157 and its biofilms was successfully developed using composite nanofiber films. The films, synthesized by encapsulating E. coli O157 phages and D-phenylalanine into sodium alginate/polyethylene oxide nanofibers, showed excellent antibacterial activity against both free E. coli O157 and biofilms. The addition of D-phenylalanine significantly enhanced the inhibitory activity against E. coli O157 proliferation and biofilm formation during short-term storage of beef and fruits and vegetables.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Review
Gastroenterology & Hepatology
Lingyan Jiang, Wen Yang, Xinlei Jiang, Ting Yao, Lu Wang, Bin Yang
Summary: EHEC O157 carries 177 unique genomic islands, termed O islands, which play a crucial role in its pathogenicity by providing virulence factors, effectors, and regulatory proteins.
Article
Microbiology
Yu-Bin Bai, Xiao-Rong Yang, Bing Li, Xu-Zheng Zhou, Wei-Wei Wang, Fu-Sheng Cheng, Ji-Yu Zhang
Summary: In this study, three AI-2 inhibitors (M414-3326, 3254-3286, and L413-0180) were obtained through virtual screening and their in vitro antibiofilm activities were validated. These inhibitors effectively inhibited biofilm formation of E. coli O157:H7 and are potential LuxS inhibitors that can be used to treat infections.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Raffaella Scotti, Bruno Casciaro, Annarita Stringaro, Fabrizio Morgia, Maria Luisa Mangoni, Roberta Gabbianelli
Summary: New strategies are needed to fight against multidrug-resistant bacteria caused by the overuse of antibiotics. Antibiofilm peptides derived from amphibian skin, such as Esc(1-21) and Esc(1-18), have shown potential to treat infections by inhibiting bacterial growth and biofilm formation. These peptides also induce the expression of genes associated with biofilm regulation and stress response in E. coli O157:H7.
Article
Agriculture, Dairy & Animal Science
Tao Yang, Zhenghui Wang, Yang Song, Xinyan Yang, Sihan Chen, Shiqian Fu, Xue Qin, Wei Zhang, Chaoxin Man, Yujun Jiang
Summary: A smartphone-based colorimetric aptasensor was developed for the detection of E. coli O157:H7 in milk, showing good reproducibility with no cross-reaction for other bacteria. The sensor could detect a concentration of 8.43 x 10^3 cfu/mL of E. coli O157:H7 in pure culture and 5.24 x 10^2 cfu/mL in artificially contaminated milk after 1 hour of incubation.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Chemistry, Analytical
Zhihua Li, Xue Zhang, Haishan Qi, Xiaowei Huang, Jiyong Shi, Xiaobo Zou
Summary: A renewable electrochemical biosensor based on mussel-inspired adhesive protein was developed for the detection of Escherichia coli O157:H7. The biosensor showed excellent repeatability and sensitivity, with a wide linear range and a low detection limit. It demonstrated good performance in detecting E. coli O157:H7 in seafood samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biotechnology & Applied Microbiology
A. Sharifi, B. Nayeri Fasaei
Summary: This study found that Thymus daenensis and Satureja hortensis essential oils have antibacterial and anti-biofilm properties against E. coli O157:H7, and also inhibit its quorum sensing system.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Lina Wu, Yunge Liu, Pengcheng Dong, Yimin Zhang, Yanwei Mao, Rongrong Liang, Xiaoyin Yang, Lixian Zhu, Xin Luo
Summary: Beef extract promoted the biofilm formation of E. coli O157:H7, with the strongest biofilm formation achieved at 37 degrees C and pH 7.0. Biofilm formation was positively correlated with autoaggregation or hydrophobicity. Gene expression varied when incubated in BE and TSB for 1 and 3 days.
JOURNAL OF FOOD PROTECTION
(2021)
Article
Biotechnology & Applied Microbiology
Linshu Lan, Hui Shi
Summary: Escherichia coli O157:H7 can be injured by lactic acid, with related genes changes in carbohydrate metabolism and osmotic stress response during sublethal injury. Recovery of injured cells involves upregulation of genes related to membrane properties, cellular processes, and virulence factors, providing insights into how the bacteria respond to stress and regain virulence.
Article
Ecology
Elisa T. Granato, William P. J. Smith, Kevin R. Foster
Summary: Bacteria can collectively defend themselves against attacks from other strains using the T6SS, and the production of EPS can serve as a collective defense mechanism to protect both EPS-producing and non-producing cells. This has been confirmed by agent-based simulations and experiments.
Article
Orthopedics
Raman Mundi, Harman Chaudhry, Seper Ekhtiari, Prabjit Ajrawat, Daniel M. Tushinski, Thomas J. Wood, Mohit Bhandari
Summary: This study conducted a systematic review and meta-analysis to assess the impact of hydrofiber dressings on reducing complications in total joint arthroplasty surgeries. The results showed that hydrofiber dressings had no observable effect on prosthetic joint infection and wound irritation, but they did reduce the rate of blisters and the number of dressing changes. However, the evidence for the reduction in blisters and dressing changes is limited due to biased trial methodologies and wide confidence intervals.
Article
Oncology
Vipin Chandra Kalia, Sanjay K. S. Patel, Byung-Kwan Cho, Thomas K. Wood, Jung-Kul Lee
Summary: Bacteria associated with the human body can survive in tumors and produce substances that can kill tumor cells, making them potential anticancer agents.
SEMINARS IN CANCER BIOLOGY
(2022)
Editorial Material
Biotechnology & Applied Microbiology
Thomas K. Wood
Summary: Reflecting on the first 15 years of Microbial Biotechnology, a shift is observed in research focus from whole-cell populations to single cells, indicating a growing interest in understanding bacterial growth in various environments. This trend instills optimism for gaining insights into the diverse aspects of bacterial growth in communities.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Ophthalmology
Thomas Charles Wood, Sundas Maqsood, Alex Saunders, William Sancha, Mayank A. Nanavaty, Michael Wearne, Saul Rajak
Summary: The objective of this study was to develop and implement a simulation-based training model for the management of posterior capsule rupture (PCR) from a non-technical skills (NTS) perspective. The results showed significant improvement in participants' NTS and non-significant improvement in their technical skills (TS).
Article
Evolutionary Biology
Silas Bossert, Thomas J. Wood, Sebastien Patiny, Denis Michez, Eduardo A. B. Almeida, Robert L. Minckley, Laurence Packer, John L. Neff, Robert S. Copeland, Jakub Straka, Alain Pauly, Terry Griswold, Sean G. Brady, Bryan N. Danforth, Elizabeth A. Murray
Summary: The mining bees (Andrenidae) are a major bee family with over 3000 species globally. Despite limited knowledge of their evolutionary history, a comprehensive genomic dataset of 195 species has revealed that their diversification rates sharply increased in the past 15 million years, especially in the genera Andrena and Perdita. This suggests that these two groups, along with the brood parasites of the genus Nomada Scopoli, are among the fastest diversifying lineages of all bees.
SYSTEMATIC ENTOMOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Thomas R. Wood, Kate Hildahl, Hawley Helmbrecht, Kylie A. Corry, Daniel H. Moralejo, Sarah E. Kolnik, Katherine E. Prater, Sandra E. Juul, Elizabeth Nance
Summary: Organotypic brain slice models are used to investigate therapeutic options for hypoxic-ischemic brain injury, a major factor in neonatal mortality. The study found highly heterogeneous regional responses to different treatments and different morphologies of microglia responded differently to injury and treatment.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Article
Medicine, General & Internal
Sandra E. Juul, Thomas R. Wood, Bryan A. Comstock, Krystle Perez, Semsa Gogcu, Mihai Puia-Dumitrescu, Sara Berkelhamer, Patrick J. Heagerty
Summary: Understanding the causes and circumstances of death in extremely preterm infants is crucial for healthcare practitioners. This study retrospectively examined a cohort of infants born between 24 and 27 weeks of gestation to determine the risk factors, causes, timing, and circumstances of death. The study found that respiratory distress or failure, pulmonary hemorrhage, necrotizing enterocolitis, catastrophic intracranial hemorrhage, sepsis, and sudden unexplained death were the primary causes of death. The risk of death decreased over time, and preterm labor was associated with a decreased hazard of death. Clinical factors such as low birth weight, low Apgar score, sick appearance at birth, necrotizing enterocolitis, pulmonary hemorrhage, severe intracranial hemorrhage, and severe sepsis were also associated with death.
Article
Agriculture, Multidisciplinary
N. Leclercq, L. Marshall, T. Weekers, A. Anselmo, D. Benda, D. Bevk, P. Bogusch, D. Cejas, B. Drepper, M. Galloni, M. Gerard, G. Ghisbain, L. Hutchinson, B. Martinet, D. Michez, J-M Molenberg, P. Nikolic, S. Roberts, G. Smagghe, J. Straka, P. Vandamme, T. J. Wood, N. J. Vereecken
AGRICULTURE ECOSYSTEMS & ENVIRONMENT
(2022)
Article
Biodiversity Conservation
Timothy Weekers, Leon Marshall, Nicolas Leclercq, Thomas J. Wood, Diego Cejas, Bianca Drepper, Michael Garratt, Louise Hutchinson, Stuart Roberts, Jordi Bosch, Laura Roquer-Beni, Patrick Lhomme, Denis Michez, Jean-Marc Molenberg, Guy Smagghe, Peter Vandamme, Nicolas J. Vereecken
Summary: This study investigates the impact of variables such as climate, landscape composition, management practices, and wild bee pollination on commercial apple production. The results show that apple yields are mainly influenced by management practices, not the contribution of managed honey bees. Wild bee diversity is negatively correlated with honey bee dominance but promotes apple quality. Thus, harnessing wild bee diversity can be a nature-based solution and a substitute for an exclusive reliance on honey bees in apple production.
ECOLOGICAL INDICATORS
(2022)
Editorial Material
Microbiology
Laura Fernandez-Garcia, Joy M. Muthami, Maria Tomas, Thomas K. Wood
ENVIRONMENTAL MICROBIOLOGY
(2022)
Editorial Material
Microbiology
Joy M. Muthami, Laura Fernandez-Garcia, Maria Tomas, Thomas K. Wood
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Agriculture, Multidisciplinary
R. N. Nichols, T. J. Wood, J. M. Holland, D. Goulson
Summary: The floral abundance and richness on farmland have been decreasing since the mid 1900s. Agri-environment schemes (AES) can help improve floral resources for insect pollinators through establishing flower-rich areas or carefully managing areas set-aside for natural regeneration. A study conducted in southern England showed that initially the sown Pollen & Nectar Strips and Florally Enhanced (FE) Grass Margins provided the greatest floral abundance, but this declined over time. Furthermore, only a few sown species known to be beneficial for insect pollinators persisted in the long term.
AGRICULTURE ECOSYSTEMS & ENVIRONMENT
(2022)
Article
Virology
Laura Fernandez-Garcia, Thomas K. Wood
Summary: The overlap between phage-defense systems (PDs) and toxin/antitoxin systems (TAs) has become clear as new PDs are discovered, as both systems use similar means to reduce cellular metabolism. They both have members that deplete energetic compounds and nucleic acids, and inflict membrane damage. Additionally, both systems reduce host metabolism to limit phage propagation and interaction of multiple defense systems.
Article
Microbiology
Laura Fernandez-Garcia, Sooyeon Song, Joy Kirigo, Michael E. Battisti, Maiken E. Petersen, Maria Tomas, Thomas K. Wood
Summary: This study reveals that bacteria can defend against phage infection by forming persister cells instead of inducing cell suicide. Furthermore, the restriction/modification systems work together with the toxin/antitoxin system to clear phage DNA. These findings are crucial for the success of phage therapy.
MICROBIOLOGY SPECTRUM
(2023)
Article
Green & Sustainable Science & Technology
Thomas K. Wood, Ilke Gurgan, Ethan T. Howley, Ingmar H. Riedel-Kruse
Summary: The anaerobic oxidation of methane (AOM) in microbial fuel cells (MFCs) shows great promise in converting methane into electricity and value-added chemicals. By integrating different technologies, the performance of AOM MFCs can be significantly improved, leading to sustainable energy production.
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
(2023)
