Article
Multidisciplinary Sciences
Shira Landau, Abigail Newman, Shlomit Edri, Inbal Michael, Shahar Ben-Shaul, Yulia Shandalov, Tom Ben-Arye, Pritinder Kaur, Ming H. Zheng, Shulamit Levenberg
Summary: Lymphatic system plays a critical role in various biological processes, yet lymphangiogenesis is less studied compared to angiogenesis. By culturing human lymphatic endothelial cells on thick collagen sheets with supporting cells and blood endothelial cells, a functional lymphatic vessel network was successfully developed in a short period of time. The model provides a potential platform for investigating lymphangiogenesis and lymphatic disease mechanisms.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Delayna Paulson, Rebecca Harms, Cody Ward, Mackenzie Latterell, Gregory J. Pazour, Darci M. Fink
Summary: Microenvironmental signals during development or inflammation can stimulate lymphatic endothelial cells to undergo lymphangiogenesis, a process involving sprouting, proliferation, and migration to expand the vascular network. Primary cilia, microtubule-based cellular protrusions housing specialized membrane receptors and signaling complexes, play a critical role in receiving extracellular cues. Disruption of primary cilia on lymphatic endothelial cells can lead to edema, increased vessel caliber and branching, as well as red blood cell-filled lymphatics, impacting lymphatic vessel patterning during development and inflammation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Marta Turati, Gianluca Mattei, Alessia Boaretto, Alberto Magi, Maura Calvani, Roberto Ronca
Summary: The lymphatic vascular system plays a crucial role in cancer progression by activating lymphatic endothelial cells and facilitating the formation of new lymphatic vessels. This study revealed that cancer-derived and microenvironmental mediators activate specific molecular pathways in lymphatic endothelial cells, leading to endothelial activation, morphogenesis, and cytokine-mediated signaling. The findings provide new insights into the lymphangiogenesis process and its relevance in different types of tumors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Peripheral Vascular Disease
Daniyal J. Jafree, David A. Long, Peter J. Scambler, Christiana Ruhrberg
Summary: Lymphatic vessels play critical roles in both health and disease, and research in this field is rapidly evolving. Recent studies have revealed new cellular mechanisms and diverse cell lineages involved in the formation of organ lymphatic vasculature.
Article
Multidisciplinary Sciences
Kazuaki Maruyama, Kazuaki Naemura, Yuichiro Arima, Yasunobu Uchijima, Hiroaki Nagao, Kenji Yoshihara, Manvendra K. Singh, Akiyoshi Uemura, Fumio Matsuzaki, Yutaka Yoshida, Yukiko Kurihara, Sachiko Miyagawa-Tomita, Hiroki Kurihara
Summary: The development of blood and lymphatic vessels around the heart is related to Semaphorin 3E (Sema3E) and its receptor PlexinD1 derived from the epicardium and pericardium. Inactivation of Sema3E-PlexinD1 signaling can improve cardiac function recovery through increasing reactive lymphangiogenesis in coronary artery diseases.
Review
Physiology
Zarah B. Tabrizi, Nada S. Ahmed, Joseph L. Horder, Sarah J. Storr, Andrew V. Benest
Summary: The lymphatic system is a crucial vascular system with diverse functions in physiology and pathology, serving as an interface between the blood vascular and immune system. While much research has focused on phenotypic switching of blood endothelium, less attention has been given to the functional and molecular heterogeneity of the lymphatic system, where transcription factor activity likely plays an important role in controlling cellular responses during lymphatic remodelling.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Medicine, Research & Experimental
Jian Chen, Fan Zhang, Mengmeng Hua, Xiaobin Song, Shaohua Liu, Zuoqing Dong
Summary: This study demonstrated a significant association between intratumoral lymphatic vessel density (ILVD) and increased lymphatic metastasis, tumor recurrence, and reduced disease-specific survival in patients with oral squamous cell carcinoma. ILVD could be considered as an indicator for predicting the prognosis of OSCC.
Article
Multidisciplinary Sciences
Esther Redder, Nils Kirschnick, Stefanie Bobe, Rene Haegerling, Nils Rouven Hansmeier, Friedemann Kiefer
Summary: The use of transgenic mouse model Vegfr3-tdTomato allows for effective visualization of lymphatic vessel structure, facilitating the study of lymphatic function and pathofunction. This mouse model inherits the reporter gene, showing stable fluorescence in lymphatic vessels across multiple organs.
Article
Biochemistry & Molecular Biology
Carina Hromada, Jaana Hartmann, Johannes Oesterreicher, Anton Stoiber, Anna Daerr, Barbara Schaedl, Eleni Priglinger, Andreas H. Teuschl-Woller, Wolfgang Holnthoner, Johannes Heinzel, David Hercher
Summary: This study reveals increased lymphangiogenesis after peripheral nerve injury and repair. The findings suggest that Schwann cells may have an inhibitory effect on lymphatic vessel formation.
Review
Physiology
Faisal Masood, Rohan Bhattaram, Mark Rosenblatt, Andrius Kazlauskas, Jin-Hong Chang, Dimitri T. Azar
Summary: Aberrant lymphatic system function is linked to various pathologies, and while lymphatic vessel regression and dysfunction can worsen some conditions, induced regression may be beneficial in certain cases. However, our understanding of lymphatic vessel regression lags behind that of blood vessel regression. By studying blood vessel regression, we can uncover hallmarks of this phenomenon that could help us better understand lymphatic vessel regression. Current research tools and models can be utilized to advance this field and gain therapeutic insights into select pathologies.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Raghu P. Kataru, Jung Eun Baik, Hyeung Ju Park, Catherine L. Ly, Jinyeon Shin, Noa Schwartz, Theresa T. Lu, Sagrario Ortega, Babak J. Mehrara
Summary: The study shows that intracellular modulation of the VEGF-C signaling pathway can induce the formation of functional lymphatic networks without off-target effects and inflammation.
Article
Oncology
Maureen Van de Velde, Marie Ebroin, Tania Durre, Marc Joiret, Lionel Gillot, Silvia Blacher, Liesbet Geris, Frederic Kridelka, Agnes Noel
Summary: This study revealed a new mechanism by which tumor exposed-LEC promote tumor cell proliferation, showcasing their role in enhancing cancer cell invasion and proliferation in vivo. The analysis of teLEC secretome identified IL6 as a key molecule modulated in teLEC, with neutralization of IL6 abolishing the mitogenic effects mediated by teLEC in vivo. The findings suggest a novel function for teLEC beyond their traditional role in lymphatic vessel formation, emphasizing their potential as key stromal determinants in future studies.
Article
Engineering, Biomedical
Kevin T. Campbell, Matthew B. Curtis, Joshua M. Massey, Kajetan Wysoczynski, Dustin J. Hadley, Steve C. George, Eduardo A. Silva
Summary: Lymphatic dysfunction is associated with the progression of several vascular disorders, and there are limited strategies currently available to promote new lymphatic vasculature. This study isolated lymphatic endothelial progenitor cells from human umbilical cord blood and found that they have the potential to promote lymphatic sprouts, highlighting the therapeutic potential of these cells. Delivery of lymphatic EPCs using alginate hydrogels also showed promise for possible therapeutic applications.
ACTA BIOMATERIALIA
(2021)
Article
Cell Biology
Lorenzo M. Fernandes, Jeffrey Tresemer, Jing Zhang, Jonathan J. Rios, Joshua P. Scallan, Michael T. Dellinger
Summary: This study reveals that activating KRAS mutations disrupt KRAS/MAPK signaling during embryonic development, leading to impaired lymphovenous valve formation and lymphatic vessel enlargement. The use of the MEK1/2 inhibitor trametinib restores normal lymphatic vessel development. These findings support the potential of MEK1/2 inhibitors for treating CLAs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Gastroenterology & Hepatology
Matthew A. Burchill, Jeffrey M. Finlon, Alyssa R. Goldberg, Austin E. Gillen, Petra A. Dahms, Rachel H. McMahan, Anne Tye, Andrew B. Winter, Julie A. Reisz, Eric Bohrnsen, Johnathon B. Schafer, Angelo D'Alessandro, David J. Orlicky, Michael S. Kriss, Hugo R. Rosen, Rebecca L. McCullough, Beth A. Jiron Tamburini
Summary: This study focuses on how normal liver functions are affected during the development of nonalcoholic steatohepatitis (NASH) and the impact of inflammatory mediators on lymphatic vasculature in the liver. The findings suggest that in NASH, there is an expansion of the lymphatic vasculature which leads to downregulation of genes essential for lymphatic identity and function. Furthermore, exposure to oxidized low density lipoprotein (oxLDL) reduces lymphatic permeability in the liver, resulting in decreased protein homeostasis and increased inflammation during chronic liver disease.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2021)
Article
Anatomy & Morphology
Kenichiro Fukuoka, Joerg Wilting, Jose Francisco Rodriguez-Vazquez, Gen Murakami, Akimitsu Ishizawa, Akio Matsubara
ANATOMICAL RECORD-ADVANCES IN INTEGRATIVE ANATOMY AND EVOLUTIONARY BIOLOGY
(2019)
Article
Cell Biology
Gesa Malik, Jeorg Wilting, Clemens Friedrich Hess, Giuliano Ramadori, Ihtzaz Ahmed Malik
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2019)
Article
Anatomy & Morphology
Shogo Hayashi, Hidetomo Hirouchi, Gen Murakami, Joerg Wilting, Jose Francisco Rodriguez-Vazquez, Ji Hyun Kim
ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
(2020)
Article
Anatomy & Morphology
Z. W. Jin, M. Yamamoto, J. H. Kim, G. Murakami, J. Wilting, J. F. Rodriguez-Vazquez
FOLIA MORPHOLOGICA
(2019)
Article
Anatomy & Morphology
Zhe Wu Jin, Shogo Hayashi, Gen Murakami, Joerg Wilting, Jose Francisco Rodriguez-Vazquez, Shinichi Abe
JOURNAL OF ANATOMY
(2020)
Article
Obstetrics & Gynecology
Juergen Becker, Gilles E. Tchagou Tchangou, Sonja Schmidt, Christina Zelent, Fritz Kahl, Joerg Wilting
BMC PREGNANCY AND CHILDBIRTH
(2020)
Article
Fisheries
Aulidya Nurul Habibah, Ahmad Reza Sharifi, Stephan Wessels, Joerg Wilting, Gabriele Hoerstgen-Schwark, Wolfgang Holtz
Summary: The study found that in Nile tilapia, thermal-treated females undergo masculinization, leading to increased body mass, without affecting ovarian dynamics in non-masculinized females. This may contribute to successful breeding of offspring and have a positive impact on global food supply.
Article
Oncology
Wilhelm Gerhard Dirks, Amanda Capes-Davis, Sonja Eberth, Silke Fahnrich, Joerg Wilting, Stefan Nagel, Laura Steenpass, Jurgen Becker
Summary: A human cell line of neuroblastic tissue thought to be lost was rediscovered and reclassified due to previous misclassification. After a long road of errors and confusion, authentic CHP-100 is now characterized as a type II EWSR1-FLI1 fusion model.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Emmad Qazi, Joerg Wilting, Neeral R. Patel, Abdullah O. Alenezi, Sean A. Kennedy, Kong T. Tan, Arash Jaberi, Sebastian Mafeld
Summary: This article reviews the embryology of lower limb arterial anatomy variations and their clinical relevance, including explanations for arterial system variations and potential complications. Understanding these variations is crucial for surgical and endovascular management.
CANADIAN ASSOCIATION OF RADIOLOGISTS JOURNAL-JOURNAL DE L ASSOCIATION CANADIENNE DES RADIOLOGISTES
(2022)
Article
Cell Biology
Juergen Becker, Sonja Schwoch, Christina Zelent, Maren Sitte, Gabriela Salinas, Joerg Wilting
Summary: Lymphedema is a chronic disease affecting millions globally, with no pharmacological treatment available. The disease is characterized by swelling, fibrosis, and collagen accumulation in affected tissues. This study found that hypoxia may contribute to fibrosis in lymphatic endothelial cells (LECs), with hypoxic LECs showing a high ability to alter the extracellular matrix.
Article
Anatomy & Morphology
Jorg Wilting, Gunther Felmerer, Jurgen Becker
Summary: Initial lymphatic vessels lack a continuous basement membrane, resulting in limited attention to the production of extracellular matrix (ECM) by lymphatic endothelial cells (LECs). This study found that under hypoxia conditions, LECs exhibited dysregulated gene expression, which may contribute to the progression of fibrosclerosis in lymphedema.
DEVELOPMENTAL DYNAMICS
(2023)
Review
Biochemistry & Molecular Biology
Joerg Wilting, Juergen Becker
Summary: Despite the long history of research on the lymphatic vascular system, there is still much unknown about its structure, function, development, and evolution. The functions of lymphatic endothelial cells are more diverse than previously thought, with implications for various physiological processes.
CELL AND BIOSCIENCE
(2022)
Article
Anatomy & Morphology
Gentaro Ishiyama, Ji Hyun Kim, Ok Hee Chai, Christoph Viebahn, Jorg Wilting, Gen Murakami, Hiroshi Abe, Shinichi Abe
Summary: The study found a combination of IAS and EAS in the anal canal, with the J-shaped portion of EAS being more easily lost in elderly Japanese individuals. This change may be an early indication of anal dysfunction.
SURGICAL AND RADIOLOGIC ANATOMY
(2021)
Article
Ophthalmology
Ji Hyun Kim, Shogo Hayashi, Masahito Yamamoto, Gen Murakami, Jorg Wilting, Jose Francisco Rodriguez-Vazquez
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2020)
Article
Multidisciplinary Sciences
Grit Lutze, Anna Haarmann, Jules A. Demanou Toukam, Kerstin Buttler, Joerg Wilting, Juergen Becker
SCIENTIFIC REPORTS
(2019)