Review
Microbiology
Su Jin Jo, Jun Kwon, Sang Guen Kim, Seung-Jun Lee
Summary: Bacterial infections have become a global threat, and bacteriophages are gaining attention as promising alternatives due to their distinctive attributes and genetic modification abilities. Phage engineering can enhance diagnostics, drug delivery, and therapy, and holds promise in transforming precision medicine, addressing antibiotic resistance, and advancing diverse applications.
Article
Biophysics
Olga I. Guliy, Stella S. Evstigneeva, Lev A. Dykman
Summary: Antibody phage display is a useful technique for producing antibodies to specific antigens, replacing the traditional hybrid-oma technology. It simplifies the process by manipulating DNA and bacteria, shortening the time and reducing the cost of antibody production. Antibodies prepared through phage display can be used as selective receptors in biosensors.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Biotechnology & Applied Microbiology
Wajid Hussain, Xiaohan Yang, Mati Ullah, Huan Wang, Ayesha Aziz, Fang Xu, Muhammad Asif, Muhammad Wajid Ullah, Shenqi Wang
Summary: Bacteriophages, the most abundant biological entity in the world, possess a vast amount of unexplored genetic information. Researchers are highly interested in phages due to their small size but ability to be engineered with desired traits. This review presents advanced strategies for developing engineered phages and explores their potential applications in various fields, such as phage therapy, biofilm disruption, antimicrobial delivery, and vaccine development, among others. The review also discusses the use of phage display-based specific peptides for targeted drug delivery and bacterial pathogen detection. Additionally, the review highlights the industrial, environmental, and medical applications of engineered phages. Overall, this review provides valuable insights into the innovative knowledge of phage engineering and its potential applications.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Microbiology
Agnieszka Klonowska, Julie Ardley, Lionel Moulin, Jaco Zandberg, Delphine Patrel, Margaret Gollagher, Dora Marinova, T. B. K. Reddy, Neha Varghese, Marcel Huntemann, Tanja Woyke, Rekha Seshadri, Natalia Ivanova, Nikos Kyrpides, Wayne Reeve
Summary: This study describes the genome features of a rhizobial strain from French Guiana, focusing on two types of prophages in its genome. STM 6018 contains a complete Mu-like capsular phage and a filamentous phage, which is the first characterization of a filamentous phage in a rhizobial strain.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemical Research Methods
Han Ying Wang, You-Chiun Chang, Che-Wei Hu, Chia-Yi Kao, Yao-An Yu, See-Khai Lim, Kurt Yun Mou
Summary: A novel therapeutic platform utilizing filamentous phage to display an antitumor cytokine was developed and demonstrated significant antitumor activities in a murine colorectal cancer model. The combined therapy of the cytokine phage and radiation greatly improved therapeutic potency, leading to 100% survival rate and 25% complete remission rate, indicating the potential for multifunctional nanoparticles for cancer therapy.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Nacyra Assad-Garcia, Roshan D'Souza, Rachel Buzzeo, Arti Tripathi, Lauren M. Oldfield, Sanjay Vashee, Derrick E. Fouts, Martha Vives
Summary: This study presents a new and efficient DNA transformation method for Staphylococcus aureus to boot-up purified phage genomic DNA and synthetic phage genomes. This method is a powerful tool for the rapid development of engineered therapeutic phages and phage cocktails against Gram-positive pathogens.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Food Science & Technology
Dan Wang, Xiangna Zhao, Haoran Wang
Summary: This study reviews recent advances in bacteriophage and derived strategies for combating biofilms in the food industry. It highlights bacteriophage engineering as an effective approach to enhance host specificity and bactericidal efficacy. Understanding and improving bacteriophage actions against biofilm contamination will strengthen food safety. This research promotes the application of bacteriophage in combating bacterial biofilms and provides insight into novel control strategies in the food industry.
Article
Multidisciplinary Sciences
Yi Yi, Shunzhang Liu, Yali Hao, Qingyang Sun, Xinjuan Lei, Yecheng Wang, Jiahua Wang, Mujie Zhang, Shan Tang, Qingxue Tang, Yue Zhang, Xipeng Liu, Yinzhao Wang, Xiang Xiao, Huahua Jian
Summary: Viruses in the oceans are found everywhere and have a high abundance and diversity. In this study, the researchers systematically analyze genomic sequences of marine prokaryotes to create two datasets: Marine Prokaryotic Genome Dataset (MPGD) consisting of over 12,000 bacterial and archaeal genomes, and Marine Temperate Viral Genome Dataset (MTVGD). They find that at least 40% of the MPGD genomes contain proviral sequences, indicating that they are lysogens. The MTVGD includes over 12,900 viral contigs or putative proviruses, clustered into 10,897 viral genera. The researchers reveal the abundance of lysogens and proviruses in marine ecosystems, especially in the deep sea, and identify differences between lysogens and non-lysogens in genomic features and growth properties. They also discover virus-host interaction networks and proviruses that can infect or transfer between different bacterial classes and phyla. The findings contribute to a better understanding of the ecology of marine prokaryotes and their viruses.
NATURE COMMUNICATIONS
(2023)
Review
Chemistry, Analytical
Ping Xu, Subhadeep Ghosh, Anam Rana Gul, Jigna R. Bhamore, Jong Pil Park, Tae Jung Park
Summary: Biosensors have seen rapid growth in the field of biological detection and biomedical diagnosis, with displayed peptides from phage display libraries being recognized as a promising alternative to antibodies. The advantages of displayed peptides include high affinity, low cost, ease of design and modification, mass production, and increased resistance to complex environments.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Cecile Philippe, Carlee Morency, Pier-Luc Plante, Edwige Zufferey, Rodrigo Achigar, Denise M. Tremblay, Genevieve M. Rousseau, Adeline Goulet, Sylvain Moineau
Summary: This study reveals that CRISPR-Cas systems in prokaryotic cells can block ACR-containing phages by targeting the acr gene, and this selection process leads to phage mutants that can interfere without acquiring new immunity. This provides an example of ACR specifically inhibiting spacer acquisition.
NATURE COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Junhua Dong, Cen Chen, Yuepeng Liu, Jingen Zhu, Mengling Li, Venigalla B. Rao, Pan Tao
Summary: Bacteriophage T4 shows great potential for biomedical applications due to its unique characteristics, though genetic engineering of its modified genome is challenging. The CRISPR-Cas12a system proves to be more efficient in cleaving the modified genome compared to the traditional CRISPR-Cas9 system, allowing for the generation of recombinant T4 phages. This CRISPR-inspired approach has the potential to expand the use of phages in various basic and translational research projects.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Luca Ulfo, Andrea Cantelli, Annapaola Petrosino, Paolo Emidio Costantini, Michela Nigro, Francesco Starinieri, Eleonora Turrini, Suleman Khan Zadran, Giampaolo Zuccheri, Roberto Saporetti, Matteo Di Giosia, Alberto Danielli, Matteo Calvaresi
Summary: Using an orthogonal nanoarchitectonics approach, M13 bacteriophages were engineered to specifically target cancer cells with EGFR overexpression, and efficiently deliver photosensitizing molecules for photodynamic therapy at picomolar concentrations.
Review
Chemistry, Medicinal
Bryan Gibb, Paul Hyman, Christine Schneider
Summary: Bacteriophages, discovered independently by Frederick Twort and Felix d'Herelle in the early 20th century, have attracted scientists' attention for over a century. With their abundance and potential in combating antibiotic-resistant pathogens, researchers are exploring various ways to modify phages for beyond their natural functions.
Review
Biochemical Research Methods
Huan Peng, Irene A. Chen
Summary: Phages are versatile agents that can deliver a variety of cargo, including nanomaterials, nucleic acids, and small molecules. They are amenable to molecular engineering, which is essential for applications such as treatment of antibiotic-resistant infections. This engineering capability may have evolutionary origins in the long-standing 'arms race' between bacteria and phages, leading to high tolerance of genetic mutations and synthetic engineering.
CURRENT OPINION IN BIOTECHNOLOGY
(2021)
Article
Microbiology
Andrzej Piekarowicz, Aneta Klyz, Daniel C. Stein
Summary: In this study, phagemid particles based on the Neisseria gonorrhoeae filamentous phage were used as a vaccine delivery system. The particles incorporated host proteins encoded by chromosomal genes or plasmids, and were prepared from three types of cells. The particles elicited a large amount of specific antibodies that recognized bacterial host cells and proteins, indicating the potential of filamentous phage as a novel immunogen delivery system.
FRONTIERS IN MICROBIOLOGY
(2022)
