期刊
CURRENT GENETICS
卷 56, 期 6, 页码 517-528出版社
SPRINGER
DOI: 10.1007/s00294-010-0319-x
关键词
Two-component; Histidine kinase; Cell wall integrity; Pathogenicity; Osmotic stress; Peroxidase activity
资金
- Commonweal Specialized Research Fund of China Agriculture [200803008]
- Natural Science Foundations of China [30771394, 30971890]
- Ministry of Education of China [NCET-07-0442]
- National 863 project of China [2008AA10Z410]
A two-component signal transduction system is a common mechanism for environmental sensing in bacteria. The functions of the two-component molecules have been also well characterized in the lower eukaryotic fungi in recent years. In Saccharomyces cerevisiae, the histidine kinase Sln1p is a major component of the two-component signaling pathways and a key regulator of the osmolarity response. To determine the function of MoSLN1, a Sln1 homolog of Magnaporthe oryzae, we cloned the MoSLN1 gene and generated specific mutants using gene knock-out strategy. Disruption of MoSLN1 resulted in hypersensitivity to various stresses, reduced sensitivity to cell wall perturbing agent Calcofluor white, and loss of pathogenicity, mainly due to a penetration defect. Additionally, we showed that MoSLN1 is involved in oxidative signaling through modulation of intra- and extracellular peroxidase activities. These results indicate that MoSLN1 functions as a pathogenicity factor that plays a role in responses to osmotic stress, the cell wall integrity, and the activity of peroxidases.
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