Review
Biochemistry & Molecular Biology
Jongmin Kim, Yeo Jin Lee, Jae Yon Won
Summary: This review highlights the roles and pathogenesis of retinal pigment epithelium (RPE) in AMD, emphasizing the importance of understanding the etiology of AMD and discussing potential treatment strategies targeting RPE.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Cody R. Fisher, Mara C. Ebeling, Zhaohui Geng, Rebecca J. Kapphahn, Heidi Roehrich, Sandra R. Montezuma, James R. Dutton, Deborah A. Ferrington
Summary: This study evaluated the treatment response of haRPE and iPSC-RPE to oxidative stress and potential therapeutics for mitochondrial defects. The results showed disease-specific responses in haRPE, while iPSC-RPE had similar responses regardless of disease status. Analysis of cells generated from the same donor showed a similar response for donors without AMD, but significant differences in treatment response between cell models from AMD donors.
Article
Multidisciplinary Sciences
Masahiro Miura, Shuichi Makita, Yoshiaki Yasuno, Takuya Iwasaki, Shinnosuke Azuma, Toshihiro Mino, Tatsuo Yamaguchi
Summary: The study quantitatively evaluated retinal pigment epithelium changes in patients with age-related macular degeneration using prototype multi-contrast optical coherence tomography. The results showed that multi-contrast OCT is useful for objective evaluation of changes in the retinal pigment epithelium.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Pei-Li Yao, Vipul M. Parmar, Mayur Choudhary, Goldis Malek
Summary: The expression and activity of NURR1 play critical roles in the physiological and pathological functions of human RPE cells. Targeting NURR1 may have therapeutic potential for AMD by improving EMT, inflammation, and lipid homeostasis in RPE cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Mara C. Ebeling, Cody R. Fisher, Rebecca J. Kapphahn, Madilyn R. Stahl, Shichen Shen, Jun Qu, Sandra R. Montezuma, Deborah A. Ferrington
Summary: This study found that the inflammasome is present and active in RPE, but it may not contribute to the pathology of early AMD.
Article
Biochemistry & Molecular Biology
Satoru Ando, Noriyasu Hashida, Daisuke Yamashita, Tsuyoshi Kawabata, Kazunobu Asao, Satoshi Kawasaki, Kazushi Sakurai, Tamotsu Yoshimori, Kohji Nishida
Summary: The study reveals that toxic fluorophore A2E impairs autophagy and upregulates Rubicon in RPE. Treatment with Rubicon-specific siRNA can reverse this impairment, and RPE-specific Rubicon deletion plays a positive role in alleviating retinal inflammation reactions.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Brandi L. Williams, Nathan A. Seager, Jamie D. Gardiner, Chris M. Pappas, Monica C. Cronin, Cristina Amat di San Filippo, Robert A. Anstadt, Jin Liu, Marc A. Toso, Lisa Nichols, Timothy J. Parnell, Jacqueline R. Eve, Paul L. Bartel, Moussa A. Zouache, Burt T. Richards, Gregory S. Hageman
Summary: Research has shown that the HTRA1 gene in the 10q26 locus is downregulated in patients with age-related macular degeneration (AMD) and this is mediated by a noncoding, cis-regulatory element overlapping the ARMS2 intron, potentially leading to an increased risk of disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Geriatrics & Gerontology
Nan Su, Uwe Hansen, Tanja Plagemann, Karin Gaeher, M. Dominik Leclaire, Jeannette Konig, Annika Hoehn, Tilman Grune, Constantin E. Uhlig, Nicole Eter, Peter Heiduschka
Summary: LF injection into the subretinal space of mice leads to activation and migration of microglial cells, increased expression of inflammatory cytokines, and gradual degradation of RPE cells. These features are consistent with those found in an aging retina, suggesting that LF injection could serve as a model for intermediate and late AMD.
Review
Biochemistry & Molecular Biology
Alexa Klettner, Johann Roider
Summary: TLR activation in the retinal pigment epithelium (RPE) can induce a pro-inflammatory response, leading to inflammation by triggering the release of pro-inflammatory cytokines, impairing RPE function, and causing RPE cell degeneration. This constant cycle of TLR activation may contribute to the development of AMD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Medicinal
Shiu-Jau Chen, Tzer-Bin Lin, Hsien-Yu Peng, Hsiang-Jui Liu, An-Sheng Lee, Cheng-Hsien Lin, Kuang-Wen Tseng
Summary: The study demonstrates that fucoxanthin protects RPE cells from oxidative stress-induced premature senescence and decreased photoreceptor cell loss. Fucoxanthin treatment significantly inhibited reactive oxygen species generation and reduced malondialdehyde concentrations in oxidative stress-induced RPE cell damage. Additionally, fucoxanthin may protect against premature senescence and cellular dysfunction in retinal cells exposed to oxidative stress.
Article
Biochemistry & Molecular Biology
Maria Hytti, Eveliina Korhonen, Heidi Hongisto, Kai Kaarniranta, Heli Skottman, Anu Kauppinen
Summary: The study showed that iPSC-RPE cells from AMD patients exhibit increased secretion of IL-1β and reduced levels of IL-6 and MCP-1 when protein clearance was partially inhibited. This suggests that dysfunctional cellular clearance mechanisms may already be present in cells originating from AMD patients, leading to inflammasome activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Neil Saptarshi, Daniel Green, Angela Cree, Andrew Lotery, Luminita Paraoan, Louise F. Porter
Summary: DNA methylation age estimation is a powerful biomarker of human ageing, and this study evaluated its association in age-related macular degeneration (AMD). Epigenetic clocks showed poor performance in retinal pigment epithelium (RPE) and epigenetic age acceleration was not observed in AMD patients. However, positive epigenetic age acceleration was observed in blood of smokers and those with AMD. Significant hypomethylation at cg04953735 within RPTOR was identified, suggesting a potential role for RPTOR as a master regulator in AMD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Shichen Shen, Rebecca J. Kapphahn, Ming Zhang, Shuo Qian, Sandra R. Montezuma, Peng Shang, Deborah A. Ferrington, Jun Qu
Summary: Age-related macular degeneration (AMD) is a leading cause of blindness in elderly individuals, but treatment options are limited. This study investigates the dysregulation of the proteome in early AMD using human donor retinal pigment epithelium (RPE). Proteomic analysis revealed significant dysregulation in biological functions and pathways, particularly in mitochondrial functions related to translation, ATP metabolism, lipid homeostasis, and oxidative stress. These findings provide valuable insights into the molecular mechanisms of early AMD and can aid in the development of treatments and biomarkers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Lara Macchioni, Davide Chiasserini, Letizia Mezzasoma, Magdalena Davidescu, Pier Luigi Orvietani, Katia Fettucciari, Leonardo Salviati, Barbara Cellini, Ilaria Bellezza
Summary: Age-related retinal degenerations, such as AMD, are linked to the loss of RPE cells and photoreceptors, involving oxidative stress and inflammatory responses. Long-term oxidative insult can induce senescence in RPE cells, affecting antioxidant response and mitochondrial functionality.
Article
Medicine, General & Internal
Junya Nagata, Satomi Shiose, Keijiro Ishikawa, Takuma Fukui, Kumiko Kano, Kenichiro Mori, Takahito Nakama, Shoji Notomi, Koh-Hei Sonoda, Tsutomu Yasukawa
Summary: This study retrospectively analyzed the clinical characteristics of RPE tears in nAMD patients and found that RPE tears were more common in typical AMD eyes with high or large PEDs and sub-RPE clefts. The visual prognosis depended on whether the RPE tear involved the fovea.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Ophthalmology
Guangpu Shi, Siqi Chen, Wambui S. Wandu, Osato Ogbeifun, Lindsey F. Nugent, Arvydas Maminishkis, Samuel J. H. Hinshaw, Ignacio R. Rodriguez, Igal Gery
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2015)
Review
Ophthalmology
Nathan A. Hotaling, Vladimir Khristov, Qin Wan, Ruchi Sharma, Balendu Shekhar Jha, Mostafa Lotfi, Arvydas Maminishkis, Carl G. Simon, Kapil Bharti
JOURNAL OF OCULAR PHARMACOLOGY AND THERAPEUTICS
(2016)
Article
Cell & Tissue Engineering
Kiyoharu J. Miyagishima, Qin Wan, Barbara Corneo, Ruchi Sharma, Mostafa R. Lotfi, Nathan C. Boles, Fang Hua, Arvydas Maminishkis, Congxiao Zhang, Timothy Blenkinsop, Vladimir Khristov, Balendu S. Jha, Omar S. Memon, Sunita D'Souza, Sally Temple, Sheldon S. Miller, Kapil Bharti
STEM CELLS TRANSLATIONAL MEDICINE
(2016)
Article
Multidisciplinary Sciences
Sami Al-Nawaiseh, Fabian Thieltges, Zengping Liu, Claudine Strack, Ralf Brinken, Norbert Braun, Marc Wolschendorf, Arvydas Maminishkis, Nicole Eter, Boris V. Stanzel
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2016)
Article
Neurosciences
Ayelen Bulloj, Arvydas Maminishkis, Masayuki Mizui, Silvia C. Finnemann
MOLECULAR NEUROBIOLOGY
(2018)
Article
Multidisciplinary Sciences
Ignacio Benedicto, Guillermo L. Lehmann, Michael Ginsberg, Daniel J. Nolan, Rohan Bareja, Olivier Elemento, Zelda Salfati, Nazia M. Alam, Glen T. Prusky, Pierre Llanos, Sina Y. Rabbany, Arvydas Maminishkis, Sheldon S. Miller, Shahin Rafii, Enrique Rodriguez-Boulan
NATURE COMMUNICATIONS
(2017)
Article
Ophthalmology
Natalie Wolkow, Yafeng Li, Arvydas Maminishkis, Ying Song, Oleg Alekseev, Jared Iacovelli, Delu Song, Jennifer C. Lee, Joshua L. Dunaief
EXPERIMENTAL EYE RESEARCH
(2014)
Article
Biochemistry & Molecular Biology
Rinki Ratnapriya, Xiaowei Zhan, Robert N. Fariss, Kari E. Branham, David Zipprer, Christina F. Chakarova, Yuri V. Sergeev, Maria M. Campos, Mohammad Othman, James S. Friedman, Arvydas Maminishkis, Naushin H. Waseem, Matthew Brooks, Harsha K. Rajasimha, Albert O. Edwards, Andrew Lotery, Barbara E. Klein, Barbara J. Truitt, Bingshan Li, Debra A. Schaumberg, Denise J. Morgan, Margaux A. Morrison, Eric Souied, Evangelia E. Tsironi, Felix Grassmann, Gerald A. Fishman, Giuliana Silvestri, Hendrik P. N. Scholl, Ivana K. Kim, Jacqueline Ramke, Jingsheng Tuo, Joanna E. Merriam, John C. Merriam, Kyu Hyung Park, Lana M. Olson, Lindsay A. Farrer, Matthew P. Johnson, Neal S. Peachey, Mark Lathrop, Robert V. Baron, Robert P. Igo, Ronald Klein, Stephanie A. Hagstrom, Yoichiro Kamatani, Tammy M. Martin, Yingda Jiang, Yvette Conley, Jose-Alan Sahel, Donald J. Zack, Chi-Chao Chan, Margaret A. Pericak-Vance, Samuel G. Jacobson, Michael B. Gorin, Michael L. Klein, Rando Allikmets, Sudha K. Iyengar, Bernhard H. Weber, Jonathan L. Haines, Thierry Leveillard, Margaret M. Deangelis, Dwight Stambolian, Daniel E. Weeks, Shomi S. Bhattacharya, Emily Y. Chew, John R. Heckenlively, Goncalo R. Abecasis, Anand Swaroop
HUMAN MOLECULAR GENETICS
(2014)
Article
Nutrition & Dietetics
Hema L. Ramkumar, Jingsheng Tuo, De F. Shen, Jun Zhang, Xiaoguang Cao, Emily Y. Chew, Chi-Chao Chan
JOURNAL OF NUTRITION
(2013)
Article
Cell Biology
Sarah L. Doyle, Ema Ozaki, Kiva Brennan, Marian M. Humphries, Kelly Mulfaul, James Keaney, Paul F. Kenna, Arvydas Maminishkis, Anna-Sophia Kiang, Sean P. Saunders, Emily Hams, Ed C. Lavelle, Clair Gardiner, Padraic G. Fallon, Peter Adamson, Peter Humphries, Matthew Campbell
SCIENCE TRANSLATIONAL MEDICINE
(2014)
Article
Cell & Tissue Engineering
Marc Ferrer, Barbara Corneo, Janine Davis, Qin Wan, Kiyoharu Joshua Miyagishima, Rebecca King, Arvydas Maminishkis, Juan Marugan, Ruchi Sharma, Michael Shure, Sally Temple, Sheldon Miller, Kapil Bharti
STEM CELLS TRANSLATIONAL MEDICINE
(2014)
Article
Biochemistry & Molecular Biology
Sanghamitra Bhattacharyya, Johnathon Sturgis, Arvydas Maminishkis, Sheldon S. Miller, Vera L. Bonilha
Summary: This study analyzed the impact of DJ-1 expression changes and oxidation of its C residues on RPE function. The results showed that decreased levels of endogenous DJ-1 resulted in increased levels of ROS, morphological changes in mitochondria structure, decreased mitochondrial membrane potential, and decreased viability of RPE cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Ophthalmology
Gabriel Luna, Geoffrey P. Lewis, Kenneth A. Linberg, Bo Chang, Quiri Hu, Peter J. Munson, Arvydas Maminishkis, Sheldon S. Miller, Steven K. Fisher
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2016)
Article
Ophthalmology
Timothy A. Blenkinsop, Janmeet S. Saini, Arvydas Maminishkis, Kapil Bharti, Qin Wan, Tina Banzon, Mostafa Lotfi, Janine Davis, Deepti Singh, Lawrence J. Rizzolo, Sheldon Miller, Sally Temple, Jeffrey H. Stern
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2015)
Article
Ophthalmology
Paul Kay, Yit C. Yang, Paul Hiscott, Donna Gray, Arvydas Maminishkis, Luminita Paraoan
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2014)