Article
Environmental Sciences
Zongyuan Zhao, Taozhong Shi, Ying Chu, Yingying Cao, Shuang Cheng, Risong Na, Yi Wang
Summary: Flupyrimin and nitenpyram, neonicotinoid insecticides, interacted with serum albumins through quenching fluorescence and forming complexes via static process, causing conformational and structure changes. Thermodynamic analysis revealed the interactions were mainly driven by hydrogen bonds and hydrophobic forces, with binding constants indicating nitenpyram had greater stability than flupyrimin in binding with albumins. The acute toxicity bioassay showed low toxicity of both insecticides to zebrafish.
Review
Food Science & Technology
Alejandra Garcia-Alonso, Marta Sanchez-Paniagua Lopez, C. Lorena Manzanares-Palenzuela, Araceli Redondo-Cuenca, Beatriz Lopez-Ruiz
Summary: Polyphenols are present in both the edible parts and by-products of vegetables, with a large proportion being metabolized in the colon by intestinal microbiota, exhibiting prebiotic-like effects. The importance of edible plant by-products as a source of polyphenols highlights the need for appropriate analytical methods to determine the total content and individual polyphenols.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Instruments & Instrumentation
Cecilia Spedalieri, Julian Plaickner, Eugen Speiser, Norbert Esser, Janina Kneipp
Summary: The UVRR spectra of BSA and HSA proteins in aqueous solution were compared to distinguish between them despite their similar amino acid composition and structure. Tryptophan residues were detected despite their low abundance, and the dominant contribution from tryptophan, tyrosine, and phenylalanine was observed in the spectra. In the high-frequency region, the signals from overtones and combination bands of phenylalanine and tyrosine were clearly observed.
APPLIED SPECTROSCOPY
(2023)
Review
Chemistry, Multidisciplinary
Oscar Vidal-Casanella, Oscar Nunez, Merce Granados, Javier Saurina, Sonia Sentellas
Summary: This article highlights the importance of phenolic acids, flavonoids, and other polyphenols as the biologically active components of nutraceuticals, and the use of liquid chromatography for their characterization and quantification. The purification of plant extracts enriched in phenolic compounds for the development of nutraceutical products is discussed.
APPLIED SCIENCES-BASEL
(2021)
Article
Biochemistry & Molecular Biology
Ankita Jalan, N. Shaemningwar Moyon
Summary: In this study, the interaction between ALP and human serum albumin (HSA) and bovine serum albumin (BSA) was investigated using various spectroscopic techniques. The results showed that ALP significantly quenched the intrinsic fluorescence of both BSA and HSA, with a noticeable red shift in emission maxima. Molecular docking studies revealed moderately strong binding affinity between ALP and BSA/HSA, primarily driven by hydrophobic forces. The spectroscopic studies also indicated that the interaction between ALP and BSA/HSA induced conformational changes.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Review
Food Science & Technology
Fang Yang, Chenyang Xie, Jie Li, Renyi Ma, Zhixiong Dang, Cunwen Wang, Tielin Wang
Summary: Polyphenol compounds, widely present in natural plants, have various functional activities such as anti-cancer and anti-inflammatory effects. Foodomics techniques, including proteomics and metabolomics, have been developed to study the mechanisms of polyphenolic activities and have made significant global research advancements.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Saumen Saha, Snehasish Bhattacharjee, Joydeep Chowdhury
Summary: This study investigated the binding interactions of the phenazinium dye Janus green blue (JGB) with human and bovine serum albumins (HSA and BSA) using multi-spectroscopic and calorimetric measurements aided by in silico calculations. The results suggested the formation of ground state complexes between JGB and the respective serum albumins, as well as conformational changes in the protein structures induced by JGB.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Review
Food Science & Technology
Mu-Fang Sun, Chang-Ling Jiang, Ya-Shuai Kong, Jin-Lei Luo, Peng Yin, Gui-Yi Guo
Summary: Polyphenols are the most important components in tea, and they determine the quality and health benefits of tea. The analysis of polyphenols in tea is crucial for understanding their nutritional value, but it is challenging due to the complexity and variety of teas. Various methods have been developed for the detection of tea polyphenols, including traditional titration and spectrophotometry, as well as newer techniques like electrochemical sensors and liquid chromatography-mass spectrometry.
Article
Chemistry, Physical
Anna Jose, Mintu Porel
Summary: A library of tuneable macromolecules was synthesized and their interaction with serum proteins was studied to understand the factors influencing protein-drug interaction. The hydrophobic character, chain length, and architecture were found to be important for a favorable interaction. The binding constant values and energy transfer efficiency were calculated, indicating a wide range of values depending on the variations in the factors. Both static and dynamic quenching were involved in the quenching mechanism of the serum protein-drug complex. Molecular docking studies supported the observed structure-activity relationship.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2023)
Review
Chemistry, Analytical
Lijuan Wang, Wenmei Zhang, Yunlong Shao, Dongtang Zhang, Guangsheng Guo, Xiayan Wang
Summary: This article reviews the methods and parameters for studying drug-protein interactions, providing important guidance and reference for drug development.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Multidisciplinary
Qing He, Xu Peng, Wanhang Jiang, Sara Toufouki, Shun Yao
Summary: This study comprehensively explores and compares the main effects of popular ionic liquids on blood for the first time. It provides key data and lays foundation for further applications and risk assessments in related fields.
GREEN CHEMISTRY LETTERS AND REVIEWS
(2022)
Review
Biochemistry & Molecular Biology
Xinhao Xu, Jinyu Hu, Huaqian Xue, Yingying Hu, Ya-nan Liu, Guanyang Lin, Liangle Liu, Ren-ai Xu
Summary: This paper reviews the applications of human and bovine serum albumin in biomedical engineering, including their structural differences, extraction methods, characteristics, functions, applications, and future research directions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Filippo Mori, Letizia Natali, Romano Danesi, Silvia Nannizzi, Claudio Farina
Summary: The study examined seven commercially available HSA preparations for clinical use and found differences in PTMs among different commercial albumins. Additionally, an increase in HSA-DA isoform and new oxidative modifications were observed in these samples at the end of the stability time.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Julita A. Talaj, Kamil Zielinski, Anna Bujacz
Summary: Serum albumin plays a crucial role in maintaining the appropriate free drug concentration in the blood. This study compares the crystal structures of albumin complexes from three different animal species with diclofenac, a commonly used non-steroidal anti-inflammatory drug. The analysis reveals both common and unique binding sites for diclofenac in these albumins, none of which overlap with those in human serum albumin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Spectroscopy
Ola Grabowska, Sergey A. Samsonov, Lech Chmurzynski, Dariusz Wyrzykowski, Krzysztof Zamojc
Summary: In this study, the binding interactions between highly negative-charged ions (hexacyanoferrates(II/III)) and bovine and human serum albumins (BSA and HSA) were investigated using various techniques. The results showed that hexacyanoferrates(II/III) effectively quenched the intrinsic fluorescence of the albumins through a static mechanism. The strength of the interactions depended on the type of albumin and could be explained by enthalpy-driven processes.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Biochemistry & Molecular Biology
Suma Pawar, Ranjita Tandel, Ramesh Kunabevu, Seetharamappa Jaldappagari
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2019)
Article
Biochemistry & Molecular Biology
Suma K. Pawar, Seetharamappa Jaldappagari
JOURNAL OF MOLECULAR RECOGNITION
(2020)
Article
Chemistry, Multidisciplinary
Roopa Naik, Suma Pawar, J. Seetharamappa
Summary: This study investigated the biomolecular binding between the potential drug ezetimibe and bovine serum albumin using spectroscopic and electrochemical methods. The results showed that hydrophobic interaction is the main factor stabilizing the interaction between ezetimibe and bovine serum albumin, while electrostatic interaction also plays a role. Additionally, the binding of ezetimibe induced conformational changes in bovine serum albumin.
Article
Biochemistry & Molecular Biology
Roopa Naik, J. Seetharamappa
Summary: This study investigated the interaction between sumatriptan and bovine serum albumin (BSA) using various molecular spectroscopic methods. The results showed that there is a moderate binding between sumatriptan and BSA, with the static quenching mechanism playing a key role and electrostatic force being crucial in the interaction. Additionally, the presence of sumatriptan improved the thermal stability of BSA and decreased the flexibility of the protein chain. Site competitive studies and molecular dynamics simulations further confirmed the location of sumatriptan in the hydrophobic cavity of site I.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Chemistry, Physical
Ayyapayya S. Mathad, J. Seetharamappa, Shankara S. Kalanur
Summary: By utilizing the advantages of beta-Cyclodextrin and neem carbon dots, a simple, eco-friendly, and sensitive electrode material was developed for electrochemical determination of the anticancer drug lapatinib. The proposed sensor showed promising applicability for the determination of lapatinib in pharmaceutical formulations and urine samples.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Biochemical Research Methods
Roopa Naik, J. Seetharamappa
Summary: This study investigates the interaction between a flavonoid and an anticancer drug GAL with DNA. The results show a groove mode of binding between GAL and DNA. This work helps to unravel the binding mechanism between GAL and DNA.
JOURNAL OF FLUORESCENCE
(2023)
Article
Materials Science, Multidisciplinary
Karuna Korgaonkar, B. S. Nishchith, S. Ashoka, J. Seetharamappa, Shankar S. Kalanur
Summary: In this study, the potential of multi-functional composites derived from rare-earth-based materials for energy storage and sensing applications was explored. Praseodymium molybdate-reduced graphene oxide [Pr0.66(MoO4)-rGO] nanocomposite was prepared and its electrochemical properties were examined. The nanocomposite showed impressive performance as a supercapacitor with high specific discharge capacitance and cycle stability. Additionally, it demonstrated enhanced electrochemical sensing of the anticancer drug silibinin, making it a promising material for various applications.
MATERIALS CHEMISTRY AND PHYSICS
(2022)
Article
Chemistry, Analytical
Ayyapayya Mathad, Karuna Korgaonkar, Seetharamappa Jaldappagari, Shankara Kalanur
Summary: Here, we present a simple, environmentally friendly, and highly sensitive electrode material, SnO2@p-rGO, for the electrochemical detection of the anti-diabetic agent repaglinide (RPG). The p-rGO nanostructure was prepared using a low-temperature solution combustion method with glycine, and the SnO2 nanoparticles were synthesized using the aqueous extract of cotton boll peel as stabilizing and reducing agents. The sensor exhibited excellent linearity in the concentration range of RPG and demonstrated high selectivity, reproducibility, and long-term stability.
Article
Chemistry, Physical
Naveenkumar P. Agadi, Karuna Korgaonkar, Ayyapayya S. Mathad, J. Seetharamappa
Summary: An eco-friendly and sensitive electrode, SnO2-beta-cyclodextrin-graphene oxide modified glassy carbon electrode, was developed for the determination of alpha-lipoic acid (ALA). SnO2 nanoparticles were prepared by the sonication method using kiwi peel extracts. The electrode exhibited enhanced anodic peak current with a 26-fold enhancement compared to the bare electrode. The sensor showed linearity in the concentration range of 6.5 x 10(-8)-7.22 x 10(-5) mol L-1 for DPV and 3.12 x 10(-8)-4.99 x 10(-5) mol L-1 for the SWV method.
Article
Chemistry, Physical
Manasa A. Doddagaddavalli, Veerendra Kumar A. Kalalbandi, Jaldappagari Seetharamappa
Summary: A series of 2,4-thiazolidinedione-phenothiazine molecular hybrids were synthesized, characterized, and screened for their antidiabetic activities. Most of the compounds exhibited significant to moderate α-amylase inhibition and glucose uptake activity. Compound 9l showed acceptable values for both α-amylase inhibition and glucose uptake assay, and its interactions with human pancreatic alpha-amylase protein and human serum albumin were investigated.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Article
Electrochemistry
Karuna Korgaonkar, Bruno G. Pollet, J. Seetharamappa, Shankara S. Kalanur
Summary: The present study synthesized eco-friendly single-phase tenorite (CuO) nanoparticles using an aqueous extract of Plectranthus scutellarioides for the first time, and these nanoparticles were combined with β-cyclodextrin (β-CD) to form a nanocomposite. The nanocomposite was then used to fabricate an electrochemical sensor for the investigation of the anticancer drug, phloretin (PHL). The electrochemical behavior of PHL was successfully studied on CuO@β-CD/GCE, and the sensor exhibited good reproducibility and stability.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2023)
Article
Materials Science, Multidisciplinary
Ayyapayya S. Mathad, Karuna Korgaonkar, J. Seetharamappa, Shankara S. Kalanur
Summary: In this study, a three-dimensional reduced porous graphene oxide-nafion nanocomposite film modified glassy carbon electrode was developed for the electrochemical determination of the flavonoid galangin. The sensor showed excellent electrocatalytic activity and high stability, with a linear response range for galangin concentration. The fabricated sensor demonstrated potential anti-interference properties and practical utility for the analysis of analyte-fortified biological samples and pharmaceutical formulations.
MATERIALS CHEMISTRY AND PHYSICS
(2023)
Article
Chemistry, Multidisciplinary
Manasa A. Doddagaddavalli, Veerendra Kumar A. Kalalbandi, T. R. Ravi Naik, Shrinivas D. Joshi, Jaldappagari Seetharamappa
Summary: In this study, a series of fluorenone-thiazolidine-4-one compounds were synthesized and characterized. The compounds were evaluated for their antioxidant and anti-diabetic properties. Some of the compounds showed promising antioxidant activity.
NEW JOURNAL OF CHEMISTRY
(2023)
Article
Chemistry, Inorganic & Nuclear
Manasa A. Doddagaddavalli, S. S. Bhat, J. Seetharamappa
Summary: A new compound, PTZ-PIP, was synthesized and characterized by various spectroscopic techniques. The molecular structure was determined by X-ray crystallography. The compound exhibited anticancer and antioxidant activity.
JOURNAL OF STRUCTURAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Roopa Naik, J. Seetharamappa
Summary: This study investigates the interaction between galangin and human serum albumin through experimental and theoretical approaches. The results suggest an interaction between them and identify the binding location of galangin on human serum albumin.
ANALYTICAL CHEMISTRY LETTERS
(2022)
