Article
Agriculture, Dairy & Animal Science
Dalton A. Holt, Isabella Alvarenga, Renan A. Donadelli, Charles G. Aldrich
Summary: This study evaluated substrate degradation and postbiotic production of several alternative fiber ingredients used in the pet food industry, and found that these alternative fiber ingredients had moderate to marginal fermentability when incubated for up to 12 hours with canine fecal inoculum.
JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Taghreed S. Alnusaire, Ibrahim Lahbib Sabouni, Hanan Khojah, Sumera Qasim, Mohammad M. Al-Sanea, Sadaf Siddique, Fatma Alzahraa Mokhtar, Shaimaa R. Ahmed
Summary: The study explores the anti-inflammatory activity of Pterocarpus dalbergioides fruit extract (PFE) and its underlying mechanism. The researchers identified 28 compounds in PFE and found that linoleic acid was the most active constituent. Network-based strategy and molecular docking studies revealed that PFE may regulate inflammation through multiple pathways. The findings suggest that PFE has potential as a drug lead for controlling inflammation.
Article
Chemistry, Physical
Hao Chen, Mengfei Huang, Wenliang Yan, Wen-Ju Bai, Xiqing Wang
Summary: Directed evolution of a P450 hydroxylase leads to a highly selective engineered enzyme capable of catalyzing the oxyfunctionalization of fatty acids. This biocatalyst displays high reactivity and can convert a variety of fatty acids to optically pure beta-hydroxy fatty acids, offering a chemoenzymatic strategy to access valuable fatty acid derivatives. Molecular docking studies provide insight into the regio- and enantioselectivity of this reaction.
Review
Biotechnology & Applied Microbiology
Junrui Wu, Chen Wu, Chunchi Rong, Jinlong Tian, Nan Jiang, Rina Wu, Xiqing Yue, Haisu Shi
Summary: This review summarizes the catalytic activities and substrate specificities of fungal fatty acid desaturases (F-FADS), highlighting the relationship between key domains and sites in F-FADS proteins and their catalytic activity. Phylogenetic analysis of the FADS cluster is also discussed, along with subcellular localization of F-FADS. Crystal structures of F-FADSs are provided as future prospects, which may aid in resolving the crystal structures of F-FADS proteins and increasing fungal PUFA production for human health.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Ankit Agrawal, Neha Bansal, Himanshu Raghav, Arijit Jana, Reena Goyal, Debashish Ghosh, Thallada Bhaskar, Bipul Sarkar
Summary: The increasing demand for sustainable energy and chemicals, along with the declining fossil fuel reserves, has led to the search for alternative feedstocks and processes. Short-chain olefins are important for the polyolefin industry and require an alternative process to meet future demands. This study demonstrates that single-cell oil (SCO) derived from the oleaginous yeast Rhodotorula mucilaginosa IIPL32 could be used as a renewable feedstock for olefin production. A PtSn/alumina catalyst showed high selectivity to C2-C4 olefins, which can be attributed to the selective catalytic cracking of long-chain lipid molecules over nanosized PtSn/alumina and the synergy between surface acidic sites and reducible Pt species.
Article
Biochemistry & Molecular Biology
Yangyang Cai, Shuqi Xing, Qifeng Zhang, Ruonan Zhu, Kai Cheng, Cuiqin Li, Xuefeng Zeng, Laping He
Summary: The study successfully expressed, purified, and characterized the extracellular lipase from Aspergillus niger GZUF36 (EXANL1), revealing its high specificity for a certain fatty acid chain length. This provides a basis for its potential applications in organic synthesis and detergent industry.
PROCESS BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Daniele Giuriato, Danilo Correddu, Gianluca Catucci, Giovanna Di Nardo, Cristiano Bolchi, Marco Pallavicini, Gianfranco Gilardi
Summary: This study designed an enzyme capable of self-producing hydrogen peroxide (H2O2) and applied it to industrial biocatalysis, effectively solving the toxicity issue of exogenous H2O2 in catalytic reactions.
Article
Biotechnology & Applied Microbiology
Jun Li, Haiqin Chen, Lulu Chang, Chen Wu, Hao Zhang, Yong Q. Chen, Wei Chen
Summary: In this study, the DGAT1 and DGAT2 isoforms from M. alpina were analyzed and heterologously expressed in S. cerevisiae H1246. MaDGAT1A/1B/2A/2B were able to restore TAG synthesis, with different substrate preferences and selectivity. This study demonstrates the potential of MaDGATs in producing TAGs rich in linoleic acid and eicosapentaenoic acid, and provides a reference for improving TAG production in oleaginous fungi.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Food Science & Technology
Zhihui Feng, Yifan Li, Ping Zhang, Jieqiong Wang, Yongquan Xu, Yingying Feng, Xiaoting Zhai, Xiaogen Yang, Xiaochun Wan, Junfeng Yin
Summary: This study investigates the synthesis of (Z)-methyl epijasmonate (epi-MeJA) and its correlation with the orchid-like aroma properties in tea during processing. It was found that epi-MeJA is mainly synthesized and accumulated during the processing of tea, with a positive correlation with processing time in the enzyme active stages. Isomerization occurs during fixation and drying stages, resulting in the formation of the less odor active isomer (Z)-methyl jasmonate.
FOOD RESEARCH INTERNATIONAL
(2023)
Review
Biotechnology & Applied Microbiology
Carmen Aranda, Juan Carro, Alejandro Gonzalez-Benjumea, Esteban D. Babot, Andres Olmedo, Dolores Linde, Angel T. Martinez, Ana Gutierrez
Summary: Selective oxyfunctionalizations of aliphatic compounds are challenging chemical reactions, but enzymes such as P450 monooxygenases and unspecific peroxygenases (UPOs) play important roles due to their stereo- and regio-selectivity. UPOs, discovered in fungi, have emerged as promising biocatalysts for their ability to catalyze the oxyfunctionalization of aliphatic and aromatic compounds using only H2O2.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Chemistry, Multidisciplinary
Mahashanon Arumugam, Chee Keong Goh, Zulkarnain Zainal, Sugeng Triwahyono, Adam F. Lee, Karen Wilson, Yun Hin Taufiq-Yap
Summary: This study presents the synthesis and application of hierarchical HZSM-5 (h-HZSM-5) catalyst with mesoporous structure, which significantly increases the yield of diesel range hydrocarbons obtained from waste oil-derived fatty acid cracking. The introduction of mesopores through silanization treatment of pre-crystallized zeolite seeds improves the accessibility of acid sites within the hierarchical network, leading to enhanced catalytic performance.
Article
Biochemistry & Molecular Biology
Mohammad Azam Ansari, Sarah Mousa Maadi Asiri
Summary: In this study, fatty acids-assisted superparamagnetic maghemite nanoparticles were biologically synthesized using extract of polyherbal drug Liv52. The nanoparticles were characterized by various techniques and were found to contain saturated and unsaturated fatty acids as well as aromatic phenol compounds. The nanoparticles exhibited antimicrobial, antibiofilm, and anticancer activities, and molecular docking studies suggested efficient interaction with cell wall components. The magnetic properties of the nanoparticles could potentially enhance their biological activity when combined with external magnetic fields or other therapeutic drugs.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Engineering, Environmental
Yafan Cai, Zehui Zheng, Xiaofen Wang
Summary: This review discusses the effects of toxicants originating from waste treatment on anaerobic digestion, particularly focusing on the activity of methanogenic archaea, the succession of MA species, and the methanogenic pathways. Under the pressure of toxicants, MA activity decreases, leading to succession among methanogens and changes in methanogenic pathways, ultimately affecting the performance of anaerobic digestion.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Environmental Sciences
Qinglian Wu, Weitong Ren, Wanqian Guo, Nanqi Ren
Summary: The study shows that combining ethanol and lactate as co-EDs can effectively increase the efficiency and yield of MCFAs synthesis, resulting in higher production and selectivity compared to using sole ED.
ENVIRONMENTAL RESEARCH
(2022)
Review
Biotechnology & Applied Microbiology
Soo-Jin Yeom, Thien-Kim Le, Chul-Ho Yun
Summary: Plastic contamination poses threats to ecosystems and wildlife species, making sustainable plastic waste management crucial. Microbial degradation of plastics, utilizing engineered P450 enzymes, can be an effective solution. The proposal suggests using these enzymes to biodegrade polyethylene and incorporating them into synthetic bacteria for plastic degradation.
TRENDS IN BIOTECHNOLOGY
(2022)