期刊
CURRENT BIOLOGY
卷 24, 期 12, 页码 1421-1428出版社
CELL PRESS
DOI: 10.1016/j.cub.2014.05.019
关键词
-
资金
- EMBL
- Deutsche Forschungsgemeinschaft (DFG) [SPP1464 EL 246/5-1, LE 2926/1-1]
- Uehara Memorial Foundation
- MIT
- National Science Foundation
Animal cells disassemble and reassemble their nuclear envelopes (NEs) upon each division [1,2]. Nuclear envelope breakdown (NEBD) serves as a major regulatory mechanism by which mixing of cytoplasmic and nuclear compartments drives the complete reorganization of cellular architecture, committing the cell for division [2, 3]. Breakdown is initiated by phosphorylation-driven partial disassembly of the nuclear pore complexes (NPCs), increasing their permeability but leaving the overall NE structure intact [4-7]. Subsequently, the NE is rapidly broken into membrane fragments, defining the transition from prophase to prometaphase and resulting in complete mixing of cyto- and nucleoplasm [6, 8]. However, the mechanism underlying this rapid NE fragmentation remains largely unknown. Here, we show that NE fragmentation during NEBD in starfish oocytes is driven by an Arp2/3 complex-nucleated F-actin shell that transiently polymerizes on the inner surface of the NE. Blocking the formation of this F-actin shell prevents membrane fragmentation and delays entry of large cytoplasmic molecules into the nucleus. We observe spike-like protrusions extending from the F-actin shell that appear to pierce the NE during the fragmentation process. Finally, we show that NE fragmentation is essential for successful reproduction, because blocking this process in meiosis leads to formation of aneuploid eggs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据