期刊
CURRENT BIOLOGY
卷 23, 期 22, 页码 2197-2207出版社
CELL PRESS
DOI: 10.1016/j.cub.2013.09.015
关键词
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资金
- CNRS
- Fondation de la Recherche Medicale (FRM)
- HFSP
- ANR-Blanc
- national infrastructure France Bio-Imaging
- Region PACA-Andor Technology and Association pour la Recherche sur le Cancer (ARC)
Background: E-cadherin plays a pivotal role in tissue morphogenesis by forming clusters that support intercellular adhesion and transmit tension. What controls E-cadherin mesoscopic organization in clusters is unclear. Results: We use 3D superresolution quantitative microscopy in Drosophila embryos to characterize the size distribution of E-cadherin nanometric clusters. The cluster size follows power-law distributions over three orders of magnitude with exponential decay at large cluster sizes. By exploring the predictions of a general theoretical framework including cluster fusion and fission events and recycling of E-cadherin, we identify two distinct active mechanisms setting the cluster-size distribution. Dynamin-dependent endocytosis targets large clusters only, thereby imposing a cutoff size. Moreover, interactions between E-cadherin clusters and actin filaments control the fission in a size-dependent manner. Conclusions: E-cadherin clustering depends on key cortical regulators, which provide tunable and local control over E-cadherin organization. Our data provide the foundation for a quantitative understanding of how E-cadherin distribution affects adhesion and might regulate force transmission in vivo.
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