Article
Biochemistry & Molecular Biology
Momoko Hayashida, Wataru Nomura, Atsushi Shiojiri, Yoshiharu Inoue
Summary: In Saccharomyces cerevisiae, the duplication and proper positioning of spindle pole bodies (SPBs) during cell cycle progression is crucial for nuclear division. This study found that the metabolite methylglyoxal (MG) derived from glycolysis perturbs the positioning of SPBs by affecting the asymmetric localization of the protein Kar9. It was also discovered that MG activates the DNA damage checkpoint pathway, which further disrupts the proper positioning of SPBs.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Oncology
Zhi Nie, Tong Pu, Zhaojie Han, Chenyang Wang, Chenglong Pan, Ping Li, Xiaoling Ma, Yanfei Yao, Youmei Zhao, Chunyan Wang, Xiulin Jiang, Jianyang Ding
Summary: This study investigated the expression level, prognostic values, diagnostic value, and immune infiltration level of Extra spindle pole bodies-like 1 (ESPL1) in lung adenocarcinoma (LUAD). The results showed that ESPL1 was upregulated in LUAD and its higher expression was associated with unfavorable prognosis and immune infiltration in LUAD. Additionally, ESPL1 expression was correlated with DNA copy number amplification and DNA hypo-methylation, and was involved in DNA replication and glycolysis signaling pathway. Knockdown of ESPL1 inhibited cell migration and invasion abilities of LUAD. These findings suggest that ESPL1 may serve as a novel prognostic biomarker for LUAD and be associated with immune cell infiltration.
FRONTIERS IN ONCOLOGY
(2022)
Article
Cell Biology
Alberto Pineda-Santaella, Nazaret Fernandez-Castillo, Alberto Jimenez-Martin, Maria del Carmen Macias-Cabeza, Angela Sanchez-Gomez, Alfonso Fernandez-Alvarez
Summary: Chromosome segregation in female meiosis in many metazoans is mediated by acentrosomal spindles, which self-assemble without the involvement of centrosomes. A recent study in fungi demonstrated the formation of self-assembled microtubule arrays capable of segregating chromosomes, shedding light on the molecular mechanisms behind acentrosomal meiosis. Comparing self-assembled spindles with canonical SPB-dependent spindles revealed both similarities and differences, with potential for further understanding gametogenesis.
JOURNAL OF CELL SCIENCE
(2021)
Article
Reproductive Biology
Yaoyao Guo, Haowei Sun, Hui Chen, Gongshe Yang, Jialun Wang, Zhengjun Qi, Weijun Pang, Guiyan Chu, Lei Gao
Summary: This study investigates the mechanism of abnormal spindle pole formation in vitrified oocytes, revealing the relationship between decreased microtubule density and reduced aMTOCs fragmentation after cryopreservation, ultimately resulting in aneuploidy.
Review
Cell Biology
Laurence Langlois-Lemay, Damien D'Amours
Summary: Centrosomes have diverse roles in addition to their well-known function as microtubule organizing centers in eukaryotic cells. They act as important structural platforms, together with their functional counterparts in lower eukaryotes, the spindle pole bodies, orchestrating signaling events essential for cell cycle progression, cellular responses to DNA damage, sensory reception, and cell homeostasis.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Silje Anda, Erik Boye, Kay Oliver Schink, Beata Grallert
Summary: Cellular asymmetry is a key factor in the ageing and evolution of multicellular organisms. Research shows that even in simple unicellular eukaryotes, the ability to distinguish 'old' from 'new' and segregate DNA asymmetrically are inherent features of cells.
Article
Genetics & Heredity
Baronger Dowell Bieger, Aysha H. Osmani, Xin Xiang, Martin J. Egan
Summary: Data presented here demonstrate that the localization of activated dynein at spindle pole bodies is cell cycle-dependent, with dynein signals associating with nuclei in early G1 but disappearing around the G1-S boundary.
FUNGAL GENETICS AND BIOLOGY
(2021)
Article
Cell Biology
Yan Li, Kiwon Song
Summary: This study found that Bfa1 can control the spindle position checkpoint (SPOC) independent of its GTPase-activating protein (GAP) activity and spindle pole body (SPB) localization, by inhibiting the activity of Tem1 to prevent premature mitotic exit.
Article
Biology
Marjan Abbasi, Alexander Julner, Yan Ting Lim, Tianyun Zhao, Radoslaw Mikolaj Sobota, Victoria Menendez-Benito
Summary: Phosphorylation of Spc110 at S11 and S36 is required for timely cell cycle progression in budding yeast. Non-phosphorylatable and double mutant Spc110 expression leads to abnormal spindle morphology and delayed cell cycle progression.
Article
Biochemistry & Molecular Biology
Anil Kumar, Sandro M. Meier, Ana-Maria Farcas, Cristina Manatschal, Yves Barral, Michel O. Steinmetz
Summary: The study reveals the crystal structure of Kar9's folded domain, showing spectrin repeats similar to other +TIPs. Mutations disrupting spectrin-repeat-mediated dimerization of Kar9 affects its distribution to microtubule tips and spindle positioning. Phosphomimetic substitution at Cdk1 sites inhibits Kar9 dimerization, displaces Kar9 from microtubules, and affects its interaction with the myosin motor Myo2.
Article
Biochemistry & Molecular Biology
Sue L. Jaspersen
Summary: The fungal kingdom is characterized by its vast diversity in ecology, life cycles, and morphology, with the spindle pole body (SPB) exhibiting significant variation among different fungi at a cellular level. Despite differences in morphology, many SPB components are conserved, pointing to commonalities in structure, function, and duplication. Advances in genomics, genetics, and cell biology have accelerated our understanding of SPB architecture in other fungi, providing insights into microtubule nucleation and other conserved processes across eukaryotes.
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Martin Wuertz, Erik Zupa, Enrico S. Atorino, Annett Neuner, Anna Boehler, Ariani S. Rahadian, Bram J. A. Vermeulen, Giulia Tonon, Sebastian Eustermann, Elmar Schiebel, Stefan Pfeffer
Summary: The study reveals the assembly mechanism of γ-TuRC and the functional role of actin in microtubule nucleation.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Alain Devault, Simonetta Piatti
Summary: The activity of the mitotic exit network (MEN) is downregulated by Amn1 protein through a dual mode of action on Tem1 GTPase, inhibiting the reset of the cell cycle clock and leading to rapid extinguishing of MEN activity.
JOURNAL OF CELL SCIENCE
(2021)
Article
Genetics & Heredity
Rosa Ramirez-Cota, Astrid N. Espino-Vazquez, Tonacy C. Rodriguez-Vega, Rocio E. Macias-Diaz, Olga A. Callejas-Negrete, Michael Freitag, Reinhard Fischer, Robert W. Roberson, Rosa R. Mourino-Perez
Summary: The spindle pole body (SPB) is the main site for microtubule nucleation and anchorage in Neurospora crassa, while the septal pore aids in the organization of the microtubule network through the anchorage of microtubule plus-ends through a pseudo-MTOC.
FUNGAL GENETICS AND BIOLOGY
(2022)
Article
Medicine, General & Internal
Rui Song, Juntao Huang, Chenglei Yang, Yuankuan Li, Guohua Zhan, Bangde Xiang
Summary: The study suggests that ESPL1 is significantly upregulated in HCC and is associated with poorer prognosis. ESPL1 and its co-expressed genes may be involved in the cell cycle and cell division in HCC.
INTERNATIONAL JOURNAL OF GENERAL MEDICINE
(2022)
