期刊
CURRENT BIOLOGY
卷 22, 期 14, 页码 1339-1343出版社
CELL PRESS
DOI: 10.1016/j.cub.2012.05.023
关键词
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资金
- National Institutes of Health (NIH)
- Yaser Abu-Mostafa Hertz Fellowship
- National Science Foundation [0758343]
- Direct For Mathematical & Physical Scien
- Division Of Physics [0848451] Funding Source: National Science Foundation
- Div Of Civil, Mechanical, & Manufact Inn
- Directorate For Engineering [0758343] Funding Source: National Science Foundation
Ever since Hershey and Chase used phages to establish DNA as the carrier of genetic information in 1952, the precise mechanisms of phage DNA translocation have been a mystery [1]. Although bulk measurements have set a time-scale for in vivo DNA translocation during bacteriophage infection, measurements of DNA ejection by single bacteriophages have only been made in vitro. Here, we present direct visualization of single bacteriophages infecting individual Escherichia con cells. For bacteriophage lambda, we establish a mean ejection time of roughly 5 min with significant cell-to-cell variability, including pausing events. In contrast, corresponding in vitro single-molecule ejections are more uniform and finish within 10 s. Our data reveal that when plotted against the amount of DNA ejected, the velocity of ejection for two different genome lengths collapses onto a single curve. This suggests that in vivo ejections are controlled by the amount of DNA ejected. In contrast, in vitro DNA ejections are governed by the amount of DNA left inside the capsid. This analysis provides evidence against a purely intrastrand repulsion-based mechanism and suggests that cell-internal processes dominate. This provides a picture of the early stages of phage infection and sheds light on the problem of polymer translocation.
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