Article
Endocrinology & Metabolism
Petrina Koumarianou, Celia Fernandez-Mendez, Danae Fajardo-Delgado, Lidia Mirella Mielu, Pilar Santisteban, Antonio De la Vieja
Summary: This study reveals the involvement of the clathrin-coated machinery in basolateral trafficking of NIS (sodium/iodide symporter), providing further understanding of NIS subcellular sorting mechanisms. These findings will help uncover new targets in thyroid cancer treatment.
Review
Neurosciences
Kate L. Prichard, Nicholas S. O'Brien, Sari R. Murcia, Jennifer R. Baker, Adam McCluskey
Summary: Endocytosis is a crucial process for cell health and signaling, specifically clathrin mediated endocytosis (CME) plays a key role in synaptic vesicle recycling and intracellular communication in the brain. This review focuses on clathrin and dynamin, two proteins involved in CME, and discusses the potential therapeutic interventions and inhibitors for conditions involving defects in CME.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Immunology
Ruihan Shi, Lei Hou, Li Wei, Jue Liu
Summary: Clathrin-mediated endocytosis serves as the initial step for effective viral infection by allowing viruses to enter cells. Adaptor proteins play a crucial role in this process, with their research shedding light on the viral life cycle and paving the way for novel therapeutics.
MICROBIAL PATHOGENESIS
(2021)
Article
Biology
Tania Lopez-Hernandez, Koh-Ichiro Takenaka, Yasunori Mori, Pornparn Kongpracha, Shushi Nagamori, Volker Haucke, Shigeo Takamori
Summary: This study found that the endocytosis of synaptic vesicles in mouse hippocampal neurons at physiological temperature is independent of clathrin, while the endocytic retrieval of certain synaptic vesicle proteins, including vesicular transporters for glutamate and GABA, relies on sorting by the clathrin adaptor AP-2.
Article
Biochemistry & Molecular Biology
Dong-Yan Song, Lin Yuan, Na Cui, Cong Feng, Lanxia Meng, Xin-He Wang, Man Xiang, Di Liu, Chun Wang, Zhentao Zhang, Jia-Yi Li, Wen Li
Summary: Parkinson's disease is an age-related chronic neurological disorder characterized by the aggregation of alpha-synuclein. This study found that over-expression of human alpha-synuclein disrupts synaptic function by interfering with vesicle recycling, but this damage can be alleviated by the re-availment of SYNJ1.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Parasitology
Constanza Feliziani, Maria R. Rivero, Gonzalo Quassollo, Andrea S. Ropolo, Maria C. Touz
Summary: This study describes the involvement of receptor-mediated endocytosis (RME) in the cell differentiation process of Giardia lamblia. Inhibition of the medium subunit Gl mu 2 of the giardial adaptin protein GlAP2 impaired RME and triggered the process of encystation in growing cells. However, blocking RME only induced cell differentiation without completing the encystation process. Furthermore, the giardial adaptin protein GlAP2 was found to be necessary for the final steps of encystation by sorting the cyst wall protein CWP1 to the plasma membrane for cyst wall formation.
INTERNATIONAL JOURNAL FOR PARASITOLOGY
(2022)
Review
Cell Biology
Sofiia Reshetniak, Silvio O. Rizzoli
Summary: The synaptic vesicle cluster is not only a storage space for synaptic vesicles, but also potentially regulates protein distribution, cytoskeleton architecture, selective removal of synaptic components, and presynaptic responses to plasticity. It serves as a key organizer of synaptic composition and dynamics.
CURRENT OPINION IN CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Wei Siao, Peng Wang, Xiuyang Zhao, Lam Dai Vu, Ive De Smet, Eugenia Russinova
Summary: Phosphorylation of the small subunit of ADAPTOR PROTEIN-2 by ADAPTOR-ASSOCIATED PROTEIN KINASE1 has an impact on Arabidopsis root tropic growth. AAK1 is involved in clathrin-mediated endocytosis regulation in mammals by phosphorylating the μ2 subunit of the AP-2 complex. Previous research found an association between AAK1 and AP-2 in Arabidopsis, but its function was unclear. In this study, genetic analysis revealed that both aak1 and ap2m mutants in Arabidopsis exhibit altered root tropic growth, including impaired touch and gravity responses. Phosphorylation of AP2M on Thr-163 by AAK1 fine-tunes endocytosis in the Arabidopsis root to control tropic growth.
Article
Biochemistry & Molecular Biology
Marine Laporte, Kwang Chi, Laura Caudal, Na Zhao, Yvonne Schwarz, Marta Rolland, Jose Martinez-Hernandez, Magalie Martineau, Christine Chatellard, Eric Denarier, Vincent Mercier, Florent Lemaitre, Beatrice Blot, Eve Moutaux, Maxime Cazorla, David J. Perrais, Fabien Lante, Dieter Bruns, Sandrine Fraboulet, Fiona Hemming, Frank Kirchhoff, Remy Sadoul
Summary: This study shows that Alix is involved in regulating bulk endocytosis at synapses during high frequency stimulation, and impairment of this process due to the lack of Alix results in abnormal synaptic recovery and plasticity.
Article
Biology
Cansu Kuey, Meghane Sittewelle, Gabrielle Larocque, Miguel Hernandez-Gonzalez, Stephen J. Royle
Summary: This study demonstrates that intracellular clathrin-coated vesicle (CCV) formation can be induced using minimal machinery in living cells, and it can occur on various membranes. The study also shows that the formation of these vesicles, called MitoPits, can faithfully recapitulate all stages of CCV formation without the involvement of accessory proteins.
Article
Clinical Neurology
Abby L. Olsen, Sabrina G. Clemens, Mel B. Feany
Summary: This study investigated the mechanism of smoking-SV2C interaction in a Drosophila model of PD, confirming a GxE interaction between nicotine and SV2, and suggesting the consideration of genetic variation in SV2C in future clinical trials on nicotine.
MOVEMENT DISORDERS
(2023)
Article
Developmental Biology
Lisa Redlingshofer, Frances M. Brodsky
Summary: The AP2 adaptor complex plays a central role in regulating clathrin-mediated endocytosis, serving as a key hub for protein interactions beyond cargo recognition and clathrin recruitment. It mediates clathrin coated pit maturation and couples lattice formation to membrane deformation. AP2 complements the attenuating role of clathrin light chain subunits in driving clathrin assembly and dynamic lattice rearrangement for budding.
CELLS & DEVELOPMENT
(2021)
Article
Cell Biology
Yi-Jheng Peng, Junhua Geng, Ying Wu, Cristian Pinales, Jennifer Langen, Yen-Ching Chang, Christopher Buser, Karen T. Chang
Summary: The study identifies Mnb and Synj as novel regulators of ADBE, and reveals that CME and ADBE are differentially governed by the dual phosphatase domains of Synj.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell Biology
Julia Solana-Balaguer, Genis Campoy-Campos, Nuria Martin-Flores, Leticia Perez-Sisques, Laia Sitja-Roqueta, Melike Kucukerden, Ana Gamez-Valero, Albert Coll-Manzano, Eulalia Marti, Esther Perez-Navarro, Jordi Alberch, Jordi Soriano, Merce Masana, Cristina Malagelada
Summary: Extracellular vesicles, specifically neuron-derived EVs, play a crucial role in intercellular communication within the central nervous system. These EVs carry signaling molecules and exert a trophic effect on neurons, influencing synaptic events and promoting neuronal protection.
JOURNAL OF EXTRACELLULAR VESICLES
(2023)
Article
Multidisciplinary Sciences
Abhijith Radhakrishnan, Xia Li, Kirill Grushin, Shyam S. Krishnakumar, Jun Liu, James E. Rothman
Summary: The controlled release of neurotransmitters stored in synaptic vesicles is essential for information processing in the brain, relying on the fusion of vesicles with presynaptic Ca2+ influx. Through cryoelectron tomography analysis, it was found that vesicles are initially tethered to the plasma membrane by protein densities under native conditions. During the process of priming for release, a highly cooperative framework is formed between the primed vesicles and the membrane, facilitating rapid neurotransmitter release following Ca2+ influx.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Cell Biology
Philip Auckland, Nicholas I. Clarke, Stephen J. Royle, Andrew D. McAinsh
JOURNAL OF CELL BIOLOGY
(2017)
Article
Cell Biology
Laura A. Wood, Gabrielle Larocque, Nicholas I. Clarke, Sourav Sarkar, Stephen J. Royle
JOURNAL OF CELL BIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Sourav Sarkar, Ellis L. Ryan, Stephen J. Royle
Article
Biochemistry & Molecular Biology
Selena G. Burgess, Manjeet Mukherjee, Sarah Sabir, Nimesh Joseph, Cristina Gutierrez-Caballero, Mark W. Richards, Nicolas Huguenin-Dezot, Jason W. Chin, Eileen J. Kennedy, Mark Pfuhl, Stephen J. Royle, Fanni Gergely, Richard Bayliss
Article
Multidisciplinary Sciences
Nicholas I. Clarke, Stephen J. Royle
NATURE COMMUNICATIONS
(2018)
Review
Cell Biology
Poonam Shah, Alexandre Chaumet, Stephen J. Royle, Frederic A. Bard
Article
Cell Biology
Gabrielle Larocque, Penelope J. La-Borde, Nicholas Clarke, Nicholas J. Carter, Stephen J. Royle
JOURNAL OF CELL BIOLOGY
(2020)
Article
Cell Biology
Cansu Kuey, Gabrielle Larocque, Nicholas I. Clarke, Stephen J. Royle
JOURNAL OF CELL SCIENCE
(2019)
Article
Cell Biology
Ellis L. Ryan, James Shelford, Teresa Massam-Wu, Richard Bayliss, Stephen J. Royle
Summary: The research identified TACC3 and clathrin as core members, and chTOG and GTSE1 as ancillary members of the complex. Additionally, PIK3C2A was shown not to be a part of the complex. Targeting the TACC3-clathrin interface or their microtubule-binding sites are the two most likely strategies to disrupt spindle stability mediated by this multiprotein complex.
JOURNAL OF CELL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Sarah M. Smith, Gabrielle Larocque, Katherine M. Wood, Kyle L. Morris, Alan M. Roseman, Richard B. Sessions, Stephen J. Royle, Corinne J. Smith
Summary: The interaction between Clathrin and the AP2 complex plays a crucial role in coated-pit assembly, with a beta 2 appendage able to bind in at least two positions in the clathrin cage. This multi-modal binding is shown to be a fundamental property of clathrin-AP2 interactions.
Article
Cell Biology
Gabrielle Larocque, Daniel J. Moore, Meghane Sittewelle, Cansu Kuey, Joseph H. R. Hetmanski, Penelope J. La-Borde, Beverley J. Wilson, Nicholas Clarke, Patrick T. Caswell, Stephen J. Royle
Summary: The study investigates the role of intracellular nanovesicles (INVs) in cell migration, showing that overexpression of TPD52-like family proteins affects cell migration, while depletion inhibits it. The research highlights the importance of proper membrane traffic regulation in cell migration and invasion, with a focus on the role of TPD52-like proteins in controlling the recycling of integrins.
JOURNAL OF CELL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Gabrielle Larocque, Stephen J. Royle
Summary: Intracellular nanovesicles (INVs) are a newly discovered class of small transport vesicles that play vital roles in cellular compartment trafficking, particularly in cell migration and invasion. This review comprehensively describes INVs, including their involvement in transport processes such as integrin recycling, and identifies key questions for future research.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell Biology
Nuria Ferrandiz, Laura Downie, Georgina P. Starling, Stephen J. Royle
Summary: Misalignment of chromosomes during mitosis can result in chromosome missegregation and the formation of micronuclei, which are associated with cancer. A study showed that chromosomes located beyond the exclusion zone become ensheathed in multiple layers of endomembranes, leading to delayed mitosis and increased frequency of chromosome missegregation and micronucleus formation. Clearance of endomembranes can rescue those chromosomes that were destined for missegregation, indicating that endomembranes promote the missegregation of misaligned chromosomes outside the exclusion zone and pose a risk factor for aneuploidy.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biology
Cansu Kuey, Meghane Sittewelle, Gabrielle Larocque, Miguel Hernandez-Gonzalez, Stephen J. Royle
Summary: This study demonstrates that intracellular clathrin-coated vesicle (CCV) formation can be induced using minimal machinery in living cells, and it can occur on various membranes. The study also shows that the formation of these vesicles, called MitoPits, can faithfully recapitulate all stages of CCV formation without the involvement of accessory proteins.
Article
Cell Biology
Me'ghane Sittewelle, Nuria Ferrandiz, Mary Fesenko, Stephen J. Royle
Summary: The biology of a cell is composed of dynamic processes, controlled by various proteins and molecules. Microscopy plays a crucial role in understanding these processes at a molecular level. Genetically encoded imaging tools, categorized into observation, inhibition, and activation strategies, have been developed to study protein function in dynamic processes. This article provides examples and a guide on using these tools to dissect protein function in cellular processes, with a focus on rapid protein modification and observing resulting cell state changes, inspiring readers for future imaging experiments.
JOURNAL OF CELL SCIENCE
(2023)