Article
Developmental Biology
Ines C. Santos, Ana M. Silva, Reto Gassmann, Ana X. Carvalho
Summary: Cytokinesis is the process in which one cell physically divides into two cells. It is driven by an equatorial contractile ring and signals from microtubule bundles. The bundling of microtubules is essential for cytokinesis. A mechanism involving the joint action of anillin and PRC1 ensures the completion of cytokinesis.
Article
Cell Biology
Mikhail Lebedev, Fung-Yi Chan, Anna Lochner, Jennifer Bellessem, Daniel S. Osorio, Elisabeth Rackles, Tamara Mikeladze-Dvali, Ana Xavier Carvalho, Esther Zanin
Summary: This study reveals a compensatory mechanism involving linear structures formed by anillin and membrane-bound NMY-2 that promotes furrowing when unbranched F-actin polymerization is compromised.
Article
Cell Biology
Imge Ozugergin, Karina Mastronardi, Chris Law, Alisa Piekny
Summary: This study uncovers the different cytokinesis kinetics and regulation mechanisms in AB cells and P-1 cells in Caenorhabditis elegans embryos.
JOURNAL OF CELL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
KangBo Ng, Nisha Hirani, Tom Bland, Joana Borrego-Pinto, Susan Wagner, Moritz Kreysing, Nathan W. Goehring
Summary: During development, the PAR polarity network adapts to changing cellular contexts and environmental cues by modifying the symmetry-breaking cues and ensuring coordination of polarity axis between neighboring cells.
Article
Cell Biology
Yujia Sun, Yongding Huang, Zhitai Hao, Shoutao Zhang, Qingnan Tian
Summary: Adult stem cells (ASCs) are pluripotent cells that can self-renew and replace lost cells. Understanding the molecular mechanisms of stem cell proliferation and cell fate decision is important for regeneration and homeostasis. In this study, researchers identified a gene called myosin regulatory light chain (MRLC) that plays a crucial role in the development of the planarian epidermis. Knockdown of MRLC leads to increased cell death, abnormal regeneration, and eventually animal death. MRLC is also involved in regulating the proliferation and differentiation of epidermal cells by cooperating with another gene called cdc42.
CELL PROLIFERATION
(2023)
Article
Biochemistry & Molecular Biology
Pankaj Kumar, Aatifa Zehra, Masum Saini, Sam J. Mathew
Summary: Myh3 gene encodes a skeletal muscle-specific contractile protein, which is crucial for proper myogenic differentiation and function. We identified a promoter-enhancer region that drives Myh3 transcription, and found that Zeb1 and Tle3 proteins are essential trans-factors that differentially regulate Myh3 expression and C2C12 cell myogenic differentiation. Tle3 functions upstream of Zeb1 in regulating myogenic differentiation, and both proteins have post-transcriptional regulatory roles in MyoG expression.
Article
Biochemistry & Molecular Biology
Pankaj Kumar, Aatifa Zehra, Masum Saini, Sam J. Mathew
Summary: Myosin heavy chain-embryonic (Myh3) is a skeletal muscle-specific protein involved in the proper differentiation and function of myogenic cells. The regulation of Myh3 expression during development and regeneration involves multiple trans-factors, including Zinc-finger E-box binding homeobox 1 (Zeb1) and Transducin-like Enhancer of Split 3 (Tle3). Zeb1 and Tle3 interact and differentially regulate Myh3 expression, with Zeb1 repressing Myh3 expression through binding to a novel E-box in the Myh3 promoter-enhancer region. Additionally, Tle3 regulates the post-transcriptional expression of MyoG through interaction with the mRNA stabilizing protein HuR.
Article
Genetics & Heredity
Elizabeth D. Drewnik, Tobias Wiesenfahrt, Ryan B. Smit, Ye-Jean Park, Linda M. Pallotto, Paul E. Mains
Summary: Actin and myosin mediate cell contractions in Caenorhabditis elegans embryos, with LET-502/Rho kinase and PAK-1/p21 activated kinase pathways triggering contraction. While lateral cells drive elongation, dorsoventral cells play a more passive role.
G3-GENES GENOMES GENETICS
(2021)
Article
Biochemistry & Molecular Biology
Abigail R. Gerhold, Joel Ryan, Julie-Nathalie Vallee-Trudeau, Jonas F. Dorn, Jean-Claude Labbe, Paul S. Maddox
Article
Developmental Biology
Patrick Narbonne, Paul S. Maddox, Jean-Claude Labbe
Review
Oncology
N. Tavernier, J. C. Labbe, L. Pintard
EXPERIMENTAL CELL RESEARCH
(2015)
Editorial Material
Cell Biology
Abigail R. Gerhold, Jean-Claude Labbe, Paul S. Maddox
DEVELOPMENTAL CELL
(2016)
Article
Cell Biology
Eugenie Goupil, Rana Amini, David H. Hall, Jean-Claude Labbe
MOLECULAR BIOLOGY OF THE CELL
(2017)
Article
Genetics & Heredity
Patrick Narbonne, Paul S. Maddox, Jean-Claude Labbe
Article
Cell Biology
Abigail R. Gerhold, Vincent Poupart, Jean-Claude Labbe, Paul S. Maddox
MOLECULAR BIOLOGY OF THE CELL
(2018)
Article
Cell Biology
Laura Benkemoun, Catherine Descoteaux, Nicolas T. Chartier, Lionel Pintard, Jean-Claude Labbe
JOURNAL OF CELL BIOLOGY
(2014)
Article
Cell Biology
Rana Amini, Eugenie Goupil, Sara Labella, Monique Zetka, Amy S. Maddox, Jean-Claude Labbe, Nicolas T. Chartier
JOURNAL OF CELL BIOLOGY
(2014)
Article
Multidisciplinary Sciences
Alexia Rabilotta, Marianne Desrosiers, Jean-Claude Labbe
Article
Cell Biology
Reda M. Zellag, Yifan Zhao, Vincent Poupart, Ramya Singh, Jean-Claude Labbe, Abigail R. Gerhold
Summary: This study investigates the technical factors affecting GSC physiology during live imaging and provides an optimized method for monitoring GSC mitosis using the CentTracker tool. The research reveals spatial clustering of mitoses within the germline tissue, but no evidence of distinct mitotic profiles within the stem cell pool.
MOLECULAR BIOLOGY OF THE CELL
(2021)
Article
Developmental Biology
Jack Bauer, Vincent Poupart, Eugenie Goupil, Ken C. Q. Nguyen, David H. Hall, Jean-Claude Labbe
Summary: The germline of C. elegans is organized as a syncytium, with each germ cell possessing an intercellular bridge maintained by a stable actomyosin ring connected to a shared cytoplasm pool called the rachis. Live imaging in first-stage larvae reveals that primordial germ cells undergo incomplete cytokinesis, with the cytokinetic ring eventually integrating with a common pool of cytoplasm termed the proto-rachis. This iterative mechanism of incomplete cytokinesis supports germline expansion and maintenance in C. elegans.
Review
Cell Biology
Abigail R. Gerhold, Jean-Claude Labbe, Ramya Singh
Summary: During animal germ line development, incomplete cytokinesis occurs after cell division, resulting in intercellular bridges that allow the exchange of cytoplasm and organelles between cells. The regulation of germ cell intercellular bridge formation has only recently started to be understood, with studies using mouse, Drosophila melanogaster, and Caenorhabditis elegans as experimental systems.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Joaquim Javary, Eugenie Goupil, Mathilde Soulez, Evgeny Kanshin, Antoine Bouchard, Ole-Morten Seternes, Pierre Thibault, Jean-Claude Labbe, Sylvain Meloche
Summary: ERK3, an atypical member of the mitogen-activated protein kinase family, has been poorly characterized in terms of its substrates and cellular functions. This study identifies supervillin (SVIL) as a direct substrate of ERK3 and demonstrates its role in regulating myosin II activation and cytokinesis completion in dividing cells. These findings unveil a new function of ERK3 in cell division and highlight its importance in the regulation of cellular processes.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Review
Pharmacology & Pharmacy
Shahriar M. Khan, Rory Sleno, Sarah Gora, Peter Zylbergold, Jean-Philippe Laverdure, Jean-Claude Labbe, Gregory J. Miller, Terence E. Hebert
PHARMACOLOGICAL REVIEWS
(2013)