Review
Oncology
Inti Peredo-Harvey, Afsar Rahbar, Cecilia Soderberg-Naucler
Summary: The study found that human cytomegalovirus (HCMV) is highly prevalent in glioblastomas and optimized immunohistochemistry techniques are required to detect it, although there are discrepancies in detection methods and results using different techniques.
Article
Biochemical Research Methods
Tian Huang, Bruno Guillotin, Ramin Rahni, Kenneth D. Birnbaum, Doris Wagner
Summary: This study developed a whole-mount RNA-FISH method for plants, which allows investigation of three-dimensional spatial gene expression patterns in entire plant tissues.
Review
Oncology
Jordana Williams, Morris Kostiuk, Vincent L. Biron
Summary: HPV is responsible for most cervical cancers and some head and neck cancers. Specific detection methods are used to diagnose HPV infections in cervical and head and neck cancers.
FRONTIERS IN ONCOLOGY
(2022)
Article
Cell Biology
Mikkel Malham, Jaslin P. James, Christian Jakobsen, Estrid Hoegdall, Kim Holmstroem, Vibeke Wewer, Boye S. Nielsen, Lene B. Riis
Summary: The study revealed a correlation between miRNA expression levels, patient age, and disease severity in UC patients, indicating a potential role of miRNAs in the regulation of inflammation in UC and their potential use as biomarkers for monitoring disease severity.
Article
Chemistry, Analytical
Yongjun Bian, Mengwei Li, Zhaojie Wu, Jian Weng, Ru Zeng, Liping Sun
Summary: Researchers have developed an ultrasensitive fluorescent biosensor using sporopollenin capsules as a substrate for isothermal amplification. This biosensor can detect miRNA with a low detection limit and a wide linear range, and it shows high selectivity and minimal interference from serum proteins.
Article
Biochemical Research Methods
Weidu Song, Chuanhao Zhang, Huichao Lin, Taiyi Zhang, Haixia Liu, Xiaowen Huang
Summary: The proposed real-time rotary PCR reaction system (RRP) meets the requirements of low cost, low-power consumption, potential for miniaturization, and portability. Experimental and theoretical results demonstrate its feasibility in the rapid quantitative detection of Pseudomonas aeruginosa and have broad application prospects in bacteria detection and food safety.
Article
Biochemical Research Methods
Shaolei Huang, Yiquan An, Bangchao Xi, Xianglian Gong, Zhongfu Chen, Shan Shao, Shengxiang Ge, Jun Zhang, Dongxu Zhang, Ningshao Xia
Summary: Nucleic acid detection is a method that directly identifies the presence of pathogenic microorganisms and has advantages of high sensitivity, commendable specificity, and a short window period. A real-time PCR system based on multiple temperature zones was proposed to solve the time-consuming problem, enabling fast detection speed and high sensitivity under extreme conditions.
Article
Chemistry, Analytical
Yixin Liu, Binxiao Li, Ya-Jun Wang, Zihui Fan, Yang Du, Bin Li, Yan-Jun Liu, Baohong Liu
Summary: In this study, enzyme-free-mediated cascade amplified nanoprobes were designed for in situ single-molecule imaging of dual-microRNAs in switchable migrating cells. The differential expression and localization of dual-miRNAs were clearly exhibited in multiple cell lines due to the enhanced sensitivity via the cascade signal amplification strategy. Moreover, in situ three-dimensional imaging of dual-miRNAs in transition of cell migration phenotypes was successfully reconstructed in both non-confined and confined microenvironments in vitro, demonstrating differential spatial distribution in a single cell. This holds great promise for exploring the key roles of spatial RNA distribution at the single-molecule level in migrating cells, and for advancing the understanding of molecular mechanisms and physical principles in 3D cell migration in vivo.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Krisztina Nikovics, Cedric Castellarin, Xavier Holy, Marjorie Durand, Halima Morin, Abdelhafid Bendahmane, Anne-Laure Favier
Summary: Understanding bone homeostasis and regeneration is of great clinical and scientific importance for the development of bone therapy. Macrophages play a crucial role in bone regeneration during healing. In situ hybridization and hybridization chain reaction can be used to explore gene expression in bone tissues.
Article
Infectious Diseases
Sheila B. Buijs, Jesper M. Weehuizen, Tim K. Jensen, Mette Boye, Mirjam H. A. Hermans, Peet T. G. A. Nooijen, Andy IM. Hoepelman, Chantal P. Bleeker-Rovers, Jan Jelrik Oosterheert, Peter C. Wever
Summary: This study assesses the value of fluorescence in situ hybridization (FISH) for detecting Coxiella burnetii in tissue samples from chronic Q fever patients. The results indicate that FISH has limited sensitivity and specificity, and its value in routine diagnostics for chronic Q fever is limited.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Genetics & Heredity
Emma Sicherre, Anne-Laure Favier, Diane Riccobono, Krisztina Nikovics
Summary: The study found that different antibodies for investigating CD206 expression may result in varied expression patterns during immunodetection, potentially leading to false results. Therefore, researchers need to carefully choose methods for characterizing macrophages. HCR is shown to be an excellent alternative method for detecting macrophages in situ.
Article
Chemistry, Multidisciplinary
Zheng Wei Wong, Jeck Fei Ng, Siu Yee New
Summary: The HCR-AgNCs nanosensor is a highly sensitive method for miR-155 detection, capable of detecting concentrations as low as 1.13 fM in buffered solution and 1.58 fM in diluted serum samples. This demonstrates great potential for clinical application.
CHEMISTRY-AN ASIAN JOURNAL
(2021)
Article
Biochemical Research Methods
Ian Dardani, Benjamin L. Emert, Yogesh Goyal, Connie L. Jiang, Amanpreet Kaur, Jasmine Lee, Sara H. Rouhanifard, Gretchen M. Alicea, Mitchell E. Fane, Min Xiao, Meenhard Herlyn, Ashani T. Weeraratna, Arjun Raj
Summary: clampFISH 2.0 is an efficient method for detecting multiple RNA species and amplifying signals simultaneously, enabling the detection of 10 different RNA species in over 1 million cells and also applicable to tissue sections.
Article
Biophysics
Ge Gao, Jin Hu, Zhen Li, Qin Xu, Cheng-Shuang Wang, Hui-Min Jia, Hong Zhou, Peng Lin, Wei-Wei Zhao
Summary: Photon-enabled bioelectronics is highly pursued in modern electronics for its non-contact, remote-control, and self-powered function in interfacing the biological world with semiconductor devices. This study demonstrates the biological regulation of surface capacitance for a new organic photoelectrochemical transistor (OPECT) biosensor, providing a generic methodology for miRNA detection and a universal mechanism for advanced OPECT bioanalytics.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Yunpeng Xu, Jiahui Chen, Xiaolu Sui, Yanzi Zhang, Aisha Zhang, Zhenyu Lin, Xinguang Liu, Jihong Chen
Summary: The study developed an ultra-sensitive biosensor for microRNA-21 (miRNA-21) by combining the high selectivity and trans-cleavage activity of CRISPR/Cas13a, the high amplification efficiency of HCR, and the high sensitivity of ECL detection. The biosensor recognized target RNA using CRISPR/Cas13a and performed trans-cleavage activity. A signal amplification was achieved by generating long dsDNAs through HCR, and ECL intensity was measured using Ru(phen)32+ as an indicator. The system showed good linear correlation and a limit of detection (LOD) of 0.53 fM for miRNA-21, and was successfully applied to detect miRNA-21 in the urine of acute kidney injury (AKI) patients.
Article
Clinical Neurology
Katarzyna Kotulska, David J. Kwiatkowski, Paolo Curatolo, Bernhard Weschke, Kate Riney, Floor Jansen, Martha Feucht, Pavel Krsek, Rima Nabbout, Anna C. Jansen, Konrad Wojdan, Kamil Sijko, Jagoda Glowacka-Walas, Julita Borkowska, Krzysztof Sadowski, Dorota Domanska-Pakiela, Romina Moavero, Christoph Hertzberg, Hanna Hulshof, Theresa Scholl, Barbora Benova, Eleonora Aronica, Jessie de Ridder, Lieven Lagae, Sergiusz Jozwiak
Summary: The EPISTOP study compared preventive and conventional antiepileptic treatment in TSC infants, finding that preventive treatment with vigabatrin was safe and effective in modifying the natural history of seizures, reducing the risk and severity of epilepsy in these infants.
ANNALS OF NEUROLOGY
(2021)
Article
Endocrinology & Metabolism
Mesut Savas, Vincent L. Wester, Bibian van der Voorn, Anand M. Iyer, Jan W. Koper, Erica L. T. van den Akker, Elisabeth F. C. van Rossum
Summary: This study found that genetic polymorphisms associated with increased glucocorticoid receptor (GR) sensitivity were related to higher risks of obesity, increased waist circumference, and metabolic syndrome compared to individuals with normal GR genes. Furthermore, this association was more pronounced in users of inhaled corticosteroids.
NEUROENDOCRINOLOGY
(2021)
Article
Pediatrics
Hanna M. Hulshof, Emma M. H. Slot, Maarten Lequin, Delphine Breuillard, Nathalie Boddaert, Sergiusz Jozwiak, Katarzyna Kotulska, Kate Riney, Martha Feucht, Sharon Samueli, Theresa Scholl, Pavel Krsek, Barbora Benova, Kees P. J. Braun, Floor E. Jansen, Rima Nabbout
Summary: Fetal cerebral lesion scores are significantly associated with neurodevelopment and autism spectrum disorder (ASD) at 2 years in children with TSC, but not with epilepsy characteristics.
JOURNAL OF PEDIATRICS
(2021)
Article
Clinical Neurology
Jessie De Ridder, Birgit Verhelle, Jan Vervisch, Katrien Lemmens, Katarzyna Kotulska, Romina Moavero, Paolo Curatolo, Bernhard Weschke, Kate Riney, Martha Feucht, Pavel Krsek, Rima Nabbout, Anna C. Jansen, Konrad Wojdan, Dorota Domanska-Pakiela, Magdalena Kaczorowska-Frontczak, Christoph Hertzberg, Cyrille H. Ferrier, Sharon Samueli, Barbora Benova, Eleonora Aronica, David J. Kwiatkowski, Floor E. Jansen, Sergiusz Jozwiak, Lieven Lagae
Summary: The timing and characteristics of the first electroencephalography (EEG) with epileptiform discharges (ED-EEG) are associated with epilepsy and neurodevelopment at 24 months in infants with tuberous sclerosis complex (TSC). Early recording of ED-EEG can help identify TSC infants at risk of severe epilepsy and those who may benefit from preventive treatment with vigabatrin.
Article
Endocrinology & Metabolism
Anela Blazevic, Anand M. Iyer, Marie-Louise F. Van Velthuysen, Johannes Hofland, Lindsey Oudijk, Wouter W. de Herder, Leo J. Hofland, Richard A. Feelders
Summary: Small-intestinal neuroendocrine tumors (SI-NETs) have a higher prevalence and worse prognosis in male patients. This study found that mesenteric metastasis and fibrosis were more common in men than women. The risk of mesenteric metastasis in women increased around menopause. The expression of estrogen receptor alpha and androgen receptor was observed in primary tumors and mesenteric metastases.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Review
Endocrinology & Metabolism
Eline van der Valk, Ozair Abawi, Mostafa Mohseni, Amir Abdelmoumen, Vincent Wester, Bibian van der Voorn, Anand Iyer, Erica van den Akker, Sanne Hoeks, Sjoerd van den Berg, Yolanda de Rijke, Tobias Stalder, Elisabeth van Rossum
Summary: The study found a positive association between glucocorticoids measured in hair and anthropometric measurements, with the relation between hair cortisone and waist circumference being the most prominent. These findings suggest an altered setpoint of the hypothalamic-pituitary-adrenal axis with increasing central adiposity.
Review
Endocrinology & Metabolism
Robin Lengton, Anand M. Iyer, Eline S. van der Valk, Ellen K. Hoogeveen, Onno C. Meijer, Bibian van der Voorn, Elisabeth F. C. van Rossum
Summary: Increasing glucocorticoid exposure is linked to weight gain, with individuals with obesity often showing high cortisol values. Individual differences in glucocorticoid sensitivity, influenced by genetic and acquired factors, play a crucial role in response to glucocorticoids and personalized treatment options targeting the glucocorticoid receptor.
Article
Endocrinology & Metabolism
Anela Blazevic, Anand M. Iyer, Marie-Louise F. van Velthuysen, Johannes Hofland, Peter M. van Koestveld, Gaston J. H. Franssen, Richard A. Feelders, Marina Zajec, Theo M. Luider, Wouter W. de Herder, Leo J. Hofland
Summary: This study found a decreased expression of tryptophan and serotonin-metabolizing enzymes in the stroma of patients with MF. This may explain the increased profibrotic potential of serotonin and the variability in the development of fibrotic complications associated with SI-NETs.
ENDOCRINE CONNECTIONS
(2022)
Article
Biochemistry & Molecular Biology
Mirte Scheper, Alessia Romagnolo, Zein Mersini Besharat, Anand M. Iyer, Romina Moavero, Christoph Hertzberg, Bernhard Weschke, Kate Riney, Martha Feucht, Theresa Scholl, Borivoj Petrak, Alice Maulisova, Rima Nabbout, Anna C. Jansen, Floor E. Jansen, Lieven Lagae, Malgorzata Urbanska, Elisabetta Ferretti, Aleksandra Tempes, Magdalena Blazejczyk, Jacek Jaworski, David J. Kwiatkowski, Sergiusz Jozwiak, Katarzyna Kotulska, Krzysztof Sadowski, Julita Borkowska, Paolo Curatolo, James D. Mills, Eleonora Aronica
Summary: This study used RNA sequencing analysis to define the expression patterns of miRNAs and isomiRs in the serum of patients with TSC, and identified potential circulating molecular biomarkers using ROC curve analysis to discriminate the development of neuropsychiatric comorbidity in TSC patients.
Review
Biochemistry & Molecular Biology
Jody M. de Jong, Diede W. M. Broekaart, Anika Bongaarts, Angelika Muhlebner, James D. Mills, Erwin A. van Vliet, Eleonora Aronica
Summary: Seizures are a common symptom of brain tumors, especially in paediatric-type tumors. The extracellular matrix plays a role in epileptogenesis and tumorigenesis. Understanding the epileptogenic properties of brain tumors is crucial for improving health outcomes and developing novel therapies.
Article
Biochemistry & Molecular Biology
Diede W. M. Broekaart, Till S. Zimmer, Sophie T. Cohen, Rianne Tessers, Jasper J. Anink, Helga E. de Vries, Jan A. Gorter, Roger Prades, Eleonora Aronica, Erwin A. van Vliet
Summary: The gelatinase inhibitor ACT-03 has shown disease-modifying effects in epilepsy models by reducing neuroinflammation and preserving blood-brain barrier integrity. Further evaluation of its effects in clinical trials is warranted.
Article
Biology
Gabriele Ruffolo, Alessandro Gaeta, Beatrice Cannata, Camilla Pinzaglia, Eleonora Aronica, Alessandra Morano, Pierangelo Cifelli, Eleonora Palma
Summary: The potential use of phytocannabinoids (pCBs), specifically cannabidiol (CBD), to treat pathological conditions has gained attention in the scientific community. CBD has shown the ability to modulate GABAergic neurotransmission and may be effective in treating pharmaco-resistant epilepsy.
Review
Medicine, General & Internal
Tessa N. A. Slagboom, Dirk Jan Stenvers, Elsmarieke van de Giessen, Stefan D. D. Roosendaal, Maartje M. L. de Win, Joseph C. J. Bot, Eleonora Aronica, Rene Post, Jantien Hoogmoed, Madeleine L. L. Drent, Alberto M. M. Pereira
Summary: The study provides a structured analysis of the diagnostic accuracy and clinical use of molecular imaging in patients with ACTH-dependent Cushing's syndrome, as well as the role of multidisciplinary counseling in decision making. It proposes a complementary diagnostic algorithm for both de novo and recurrent or persistent Cushing's disease. The use of molecular imaging modalities is of added and complementary value in the detection of pituitary microadenoma in ACTH-dependent Cushing's syndrome.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Cell Biology
Valentina La Cognata, Agata Grazia D'Amico, Grazia Maugeri, Giovanna Morello, Maria Guarnaccia, Benedetta Magri, Eleonora Aronica, Velia D'Agata, Sebastiano Cavallaro
Summary: Amyotrophic lateral sclerosis (ALS) is characterized by motor neuron (MN) depletion. CXCR2 has been implicated in ALS pathology and its expression is increased in cortical neurons of sporadic ALS patients. Inhibition of CXCR2 prevents MN degeneration in vitro and improves neuromuscular function in SOD1-G93A mice.