Article
Multidisciplinary Sciences
Ketaki D. Belsare, Haifan Wu, Dibyendu Mondal, Annalise Bond, Erika Castillo, Jia Jin, Hyunil Jo, Addison E. Roush, Kala Bharath Pilla, Andrej Sali, Carlo Condello, William F. DeGrado
Summary: The soluble form of TREM2, sTREM2, was found to bind to fibrillar A1340 and A1342, enhancing their uptake into cells. sTREM2 inhibited the secondary nucleation step in A13 fibrillization, but had little effect on the primary nucleation pathway. The disease-associated mutant R47H showed reduced uptake and functional responses to fibrils. Molecular modeling revealed the binding mode of sTREM2 to A13 fibrils.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Neurosciences
Jacob M. Basak, Aura Ferreiro, Lucy S. Cohen, Patrick W. Sheehan, Collin J. Nadarajah, Michael F. Kanan, Kimberley V. Sukhum, Gautam Dantas, Erik S. Musiek
Summary: The study reveals that sepsis may exacerbate amyloid plaque deposition and plaque-related inflammation, potentially leading to increased dementia in older sepsis survivors.
NEUROBIOLOGY OF DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Willy Smeralda, Marc Since, Julien Cardin, Sophie Corvaisier, Sophie Lecomte, Christophe Cullin, Aurelie Malzert-Freon
Summary: The study focused on the molecular interactions between the amyloid beta peptide associated with Alzheimer's disease and biological membranes, developing simple liposomal formulations mimicking neuronal cell membranes. Characterization of interactions through a multiparametric procedure laid the methodological foundation for developing an original model describing interactions between A beta peptide and lipids.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Neurosciences
Abdullah Al Bashit, Prakash Nepal, Theresa Connors, Derek H. Oakley, Bradley T. Hyman, Lin Yang, Lee Makowski
Summary: Alzheimer's disease is a neurodegenerative disorder characterized by the formation and spread of fibrillar aggregates of A beta peptide and tau protein. By using scanning x-ray microdiffraction, researchers have successfully mapped the distribution of these aggregates in human brain tissue, providing insights into the molecular mechanisms underlying the disease.
FRONTIERS IN NEUROSCIENCE
(2022)
Article
Chemistry, Physical
Tran Thi Minh Thu, Mai Suan Li
Summary: The formation of fibrillar structure of amyloid proteins/peptides is associated with neurodegenerative diseases. Mechanical stability is found to be related to the rate of protein aggregation, with higher mechanical stability leading to faster fibril formation.
JOURNAL OF CHEMICAL PHYSICS
(2022)
Article
Chemistry, Multidisciplinary
Soumen Kuila, Sukantha Dey, Pijush Singh, Akash Shrivastava, Jayanta Nanda
Summary: This article summarizes the major findings regarding the formation of phenylalanine-based fibrils in aqueous and organic media and discusses the role of non-covalent interactions in the self-assembly process. It is found that phenylalanine-based fibrils are toxic and can induce other neurodegenerative peptides to form toxic fibrils. The article also discusses different approaches to inhibit fibril formation and reduce its toxicity.
CHEMICAL COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Oxana V. Galzitskaya, Sergei Y. Grishin, Anna V. Glyakina, Nikita V. Dovidchenko, Anastasiia V. Konstantinova, Sergey V. Kravchenko, Alexey K. Surin
Summary: In recent years, there has been an increase in the number of identified diseases associated with the accumulation of amyloid proteins, which are known to cause degenerative diseases in humans. Therefore, it is important to develop strategies for the search and development of effective inhibitors of amyloid formation. This review focuses on three amyloidogenic peptides and proteins - A beta, alpha-synuclein, and insulin - and analyzes existing and prospective strategies for the development of effective and non-toxic inhibitors of amyloid formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Siddhartha Banerjee, Mohtadin Hashemi, Karen Zagorski, Yuri L. Lyubchenko
Summary: The presence of cholesterol in lipid bilayers significantly enhances the aggregation process of Aβ42 at low concentrations, indicating that the lipid composition plays a crucial role in controlling the self-assembly of Aβ oligomers in cellular membranes.
ACS CHEMICAL NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Lei Gu, Zhefeng Guo
Summary: Formation of amyloid oligomers and fibrils, underlying neurodegenerative diseases like Alzheimer's, involves interactions with cellular membranes. The conversion of Aβ42 globulomers to fibrils in the presence of DOPC liposomes suggests a dynamic nature of interactions between Aβ oligomers and membranes. Lipid membranes can reduce membrane-disrupting activities caused by Aβ oligomers by converting them to fibrils.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Medicine, General & Internal
Giulia Bivona, Matilda Iemmolo, Tommaso Piccoli, Luisa Agnello, Bruna Lo Sasso, Marcello Ciaccio, Giulio Ghersi
Summary: Alzheimer's disease (AD) is the most common form of cognitive decline, characterized by aggregates of A beta and tau protein. In addition to A beta deposition, inflammation and microglia activation in the brain may also play a role in the pathogenesis of AD.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Mohtadin Hashemi, Siddhartha Banerjee, Yuri L. Lyubchenko
Summary: The effects of membranes on the early-stage aggregation of amyloid beta (A beta) have been studied, showing that direct A beta-membrane interactions dramatically enhance the aggregation process. Cholesterol in membranes significantly enhances the aggregation kinetics and accelerates the formation and dissociation of aggregates. Cholesterol binds A beta monomers and changes their conformation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Clinical Neurology
Ling-Zhi Ma, Hao Hu, Zuo-Teng Wang, Ya-Nan Ou, Qiang Dong, Lan Tan, Jin-Tai Yu
Summary: The study found that early accumulation of A beta protein in Alzheimer's disease has an independent effect on cognitive decline in normal controls, but has a tau and neurodegeneration-dependent effect on subsequent cognitive decline in patients with mild cognitive impairment.
ALZHEIMERS RESEARCH & THERAPY
(2021)
Article
Multidisciplinary Sciences
Dorin Sade Yazdi, Dana Laor Bar-Yosef, Hanaa Adsi, Topaz Kreiser, Shahaf Sigal, Santu Bera, Dor Zaguri, Shira Shaham-Niv, Damilola S. Oluwatoba, Davide Levy, Myra Gartner, Thanh D. Do, Dan Frenkel, Ehud Gazit
Summary: High levels of homocysteine have been linked to Alzheimer's disease. Metabolites may play a significant role in the pathogenesis of neurodegenerative diseases. Experimental evidence from yeast and mouse models suggests a molecular basis for the association between homocysteine and Alzheimer's disease pathology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Kelly Ceyzeriat, Thomas Zilli, Philippe Millet, Nikolaos Koutsouvelis, Giovanna Dipasquale, Christine Fossey, Thomas Cailly, Frederic Fabis, Giovanni B. B. Frisoni, Valentina Garibotto, Benjamin B. B. Tournier
Summary: Low-dose brain radiation therapy has been shown to reduce neuroinflammation, lower amyloid levels, and improve cognitive performances in animal models of Alzheimer's disease.
JOURNAL OF NEUROINFLAMMATION
(2022)
Review
Biochemistry & Molecular Biology
Giuseppe Di Natale, Giuseppina Sabatino, Michele Francesco Maria Sciacca, Rita Tosto, Danilo Milardi, Giuseppe Pappalardo
Summary: In the last two decades, the amyloid hypothesis has been the mainstream concept in Alzheimer's Disease research. However, recent evidence suggests that tau accumulation is more closely related to cognitive decline and AD development. Failed clinical trials of amyloid-targeting drug candidates imply the need for significant amendments to the amyloid hypothesis. This article discusses the potential dangerous relationships between A beta oligomeric species and tau protein and explores the molecular determinants underlying their cross interactions.
Article
Biophysics
Jiri Smejkal, Petr Maly, Milan Kuchar, Natalya Panova, Alena Semeradtova, Petr Aubrecht, Marcel Stofik, Jan Maly
Summary: Cell immunocapture microfluidic devices can efficiently capture cells by utilizing small recombinant protein binders (PBs) that bind to cell surface receptors, showing potential applications in medical diagnostics. The study demonstrates the effectiveness of PBs in capturing cells with high affinity and selectivity, providing a flexible and cost-effective method for rare cell immunocapture.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biochemistry & Molecular Biology
Elena Alvarez-Marimon, Hiram Castillo-Michel, Juan Reyes-Herrera, Jofre Seira, Ester Aso, Margarita Carmona, Isidre Ferrer, Josep Cladera, Nuria Benseny-Cases
Summary: Amyloid plaques in the cortex of patients with advanced Alzheimer's disease have been characterized into two types: fibrillary plaques with iron accumulation mainly in Fe2+ form, and nonfibrillary plaques with a mixture of beta-sheet and unordered structures where Fe3+ predominates over Fe2+. This distinction in plaque types and metal composition could play a key role in understanding the progression of Alzheimer's disease.
ACS CHEMICAL NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Monika Mullerova, Dina Maciel, Nadia Nunes, Dominika Wrobel, Marcel Stofik, Lucie Cervenkova St Astna, Alena Krupkova, Petra Curinova, Katerina Novakova, Matej Bozik, Marek Maly, Jan Maly, Joao Rodrigues, Tomas Strasak
Summary: This study presents a stable glycodendrimer (glyco-DDM) series with excellent biocompatibility and potential applications in drug delivery, particularly showing promising results in encapsulating anticancer agents. The glyco-DDM/DOX complexes exhibit gradual and pH-dependent release profiles, preferential internalization in cancer cells, and promising cytotoxic effects on cancer cell lines. The results suggest that novel glycoDDMs and their drug complexes hold great promise in drug delivery and related applications.
Article
Biochemistry & Molecular Biology
Antonin Edr, Dominika Wrobel, Alena Krupkova, Lucie Cervenkova Stastna, Petra Curinova, Ales Novak, Jan Maly, Jitka Kalasova, Marek Maly, Tomas Strasak
Summary: This report focuses on the development of versatile synthetic protocols for amphiphilic carbosilane dendrons (amp-CS-DDNs) that can self-assemble into regular micelles and other supramolecular objects. The presented strategy allows for fine modification of the amphiphilic structure and easy connection of desired functionalities. Experimental results demonstrate correlations between structural parameters of amp-CS-DDNs and the size of formed nanoparticles, while computer simulation models provide detailed information about the organization and spatial distribution of amp-CS-DDNs assemblies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Olga Janouskova, Regina Herma, Alena Semeradtova, David Poustka, Michaela Liegertova, Hana Auer Malinska, Jan Maly
Summary: This review provides a brief summary of conventional and nonconventional sources of extracellular vesicles (EVs), storage conditions, isolation methods, and their significance in biomedical applications, especially in diagnostics, therapy, aging, and COVID-19-related issues.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Review
Physiology
Michaela Kocholata, Jan Maly, Jan Martinec, Hana A. U. E. R. Malinska
Summary: Extracellular vesicles are small membrane particles released by organisms and can play a role in communication and regulation of gene expression and physiological processes.
PHYSIOLOGICAL RESEARCH
(2022)
Article
Multidisciplinary Sciences
Michaela Liegertova, Alena Semeradtova, Michaela Kocholata, Michaela Prusova, Lenka Nemcova, Marcel Stofik, Sylvie Krizenecka, Jan Maly, Olga Janouskova
Summary: This study focused on the isolation and characterization of extracellular vesicles, specifically exosomes and exosome-like vesicles, from slug secretions. The isolated vesicles carry abundant proteins and short RNAs, as well as adhesion molecules similar to mammalian galectins. They are internalized by plant cells and human cancer cells in vitro and can be loaded with bioactive compounds, making them an interesting tool for biotechnological applications.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Michaela Kocholata, Michaela Prusova, Hana Auer Malinska, Jan Maly, Olga Janouskova
Summary: This study focuses on the isolation of plant extracellular vesicles (pEVs) from tobacco, using two different methods. The results show that pEVs isolated by different methods have different properties and are capable of entering both plant and mammalian cells.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Mariapompea Cutroneo, Vladimir Hnatowicz, Anna Mackova, Petr Malinsky, Romana Miksova, Giovanni Ceccio, Jan Maly, Jiri Smejkal, Marcel Stofik, Vladimir Havranek
Summary: This study focuses on using low-energy ions to fabricate membranes with higher surface density and negligible overlapping pores. The results have potential applications in fields such as microfluidics and organ-on-chip microsystems.
Article
Biophysics
Jiri Smejkal, Petr Aubrecht, Alena Semeradtova, Marcel Stofik, Michaela Liegertova, Jan Maly
Summary: Cell immunocapture microsystems are an emerging field with potential medical diagnostic applications for isolating and quantifying circulating rare cells (CRCs). A simple and robust stop-flow microsystem based on planar antibody-coated surfaces was designed and achieved up to 90% immunocapture efficiency of MCF-7 cells in whole blood. The results can contribute to the design of microsystems for the isolation and identification of rare cells from blood.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Multidisciplinary Sciences
Kamila Novotna Floriancicova, Athanasios Baltzis, Jiri Smejkal, Michaela Czernekova, Lukasz Kaczmarek, Jan Maly, Cedric Notredame, Stanislav Vinopal
Summary: Tardigrades, also known as water bears, are able to survive extreme environmental conditions through reversible morphological changes and cryptobiosis. In this study, the molecular composition of tardigrade tubulins, which are important components of the microtubule cytoskeleton, was analyzed and characterized. The results support the phylogenetic placement of tardigrades within the Panarthropoda clade.
SCIENTIFIC REPORTS
(2023)
Article
Materials Science, Multidisciplinary
Mariapompea Cutroneo, Lorenzo Torrisi, Letteria Silipigni, Vladimir Havranek, Anna Mackova, Petr Malinsky, Romana Miksova, Jan Maly, Marcel Stofik, Petr Aubrecht, Dominik Fajstavr, Petr Slepicka
Summary: A cyclic olefin copolymer (COC) is a thermoplastic polymer with glass-like optical transparency, unique biocompatibility, low autofluorescence, good chemical stability, and excellent water vapor barriers. COC shows great potential for optics, medical devices, nanotechnology, and microelectronics. The modification of COC through techniques such as plasma treatment and lithography determines its applicability.
Article
Materials Science, Multidisciplinary
M. Cutroneo, L. Silipigni, A. Torrisi, A. Mackova, P. Malinsky, R. Miksova, J. Maly, M. Stofik, P. Slepicka, D. Fajstavr, V. Holy, M. Dopita, L. Torrisi
Summary: Measurements of nitrogen and argon diffusion coefficient in cyclic olefin copolymer (COC) were conducted at different temperatures. Optical transparent COC foils were prepared and characterized by SEM and AFM. The measured diffusion coefficients at room temperature were 6.56 x 10-3 cm2/s for N2 and 6.30 x 10-3 cm2/s for Ar, which showed a significant increase with temperature. The high diffusion coefficients were attributed to the amorphous nature, nano-porous structure, and damage suffered by the COC foils under high applied pressure. The experimental setup, results, COC microstructure, comparison with literature, and potential biomedical applications were presented and discussed.
Article
Chemistry, Multidisciplinary
Petr Panuska, Zuzana Nejedla, Jiri Smejkal, Petr Aubrecht, Michaela Liegertova, Marcel Stofik, Jaromir Havlica, Jan Maly
Summary: This study presented a novel millifluidic chip fabricated by 3D printing technology for long-term zebrafish embryo cultivations and toxicity testing, which can be further developed for automated assays. The chip design includes unique functionality for individual embryo removal, allowing for detailed studies of selected embryos by additional methods. Long-term perfusion cultivation experiments showed normal development of zebrafish embryos in the chip, with increased toxic effects observed with ethanol as a teratogen compared to standard FET assays.
Article
Chemistry, Multidisciplinary
Zuzana Nejedla, David Poustka, Regina Herma, Michaela Liegertova, Marcel Stofik, Jiri Smejkal, Vaclav Sicha, Pavel Kaule, Jan Maly
Summary: This study tested the toxicity of E-Shell 300 photopolymer in in vivo and in vitro biological models, revealing negative impacts and the potential for elimination through post-processing. The results highlight the importance of biocompatibility testing for 3D printing photopolymer materials and the potential for broader applications in developing complex microfluidic devices.
