期刊
JOURNAL OF NEUROCHEMISTRY
卷 136, 期 2, 页码 276-284出版社
WILEY
DOI: 10.1111/jnc.13407
关键词
elongation; mass spectrometry; microtubules; primary neurons; SILAC; translational control
资金
- ERANET systems biology (SYNSYS) ISF [1003/12]
- BBSRC [BB/I004483/1]
- Wellcome Trust [086688]
- Canadian Institutes of Health Research [MOP-77688]
- Biotechnology and Biological Sciences Research Council [BB/I004483/1]
- Biotechnology and Biological Sciences Research Council [BB/I004483/1] Funding Source: researchfish
- BBSRC [BB/I004483/1] Funding Source: UKRI
Modulation of the elongation phase of protein synthesis is important for numerous physiological processes in both neurons and other cell types. Elongation is primarily regulated via eukaryotic elongation factor 2 kinase (eEF2K). However, the consequence of altering eEF2K activity on the synthesis of specific proteins is largely unknown. Using both pharmacological and genetic manipulations of eEF2K combined with two protein-labeling techniques, stable isotope labeling of amino acids in cell culture and bio-orthogonal non-canonical amino acid tagging, we identified a subset of proteins whose synthesis is sensitive to inhibition of eEF2K in murine primary cortical neurons. Gene ontology (GO) analyses indicated that processes related to microtubules are particularly sensitive to eEF2K inhibition. Our findings suggest that eEF2K likely contributes to neuronal function by regulating the synthesis of microtubule-related proteins.
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