Article
Agriculture, Dairy & Animal Science
Sahar Abdalkarim Salih, Hossein Daghigh-Kia, Mahdieh Mehdipour, Abouzar Najafi
Summary: The study showed that supplementing the semen extender with 5 and 10 mu M of ergothioneine, and thawing the sperm at 60 degrees C for 5 seconds, can be an effective strategy to preserve the quality of cryopreserved rooster semen, as it resulted in better sperm motility, membrane integrity, and mitochondrial activity.
Article
Multidisciplinary Sciences
Martina Ahlberg, Sunjae Chung, Sheng Jiang, Andreas Frisk, Maha Khademi, Roman Khymyn, Ahmad A. Awad, Q. Tuan Le, Hamid Mazraati, Majid Mohseni, Markus Weigand, Iuliia Bykova, Felix Gross, Eberhard Goering, Gisela Schutz, Joachim Grafe, Johan Akerman
Summary: Magnetic droplets can be frozen into static nanobubbles by decreasing the applied magnetic field.
NATURE COMMUNICATIONS
(2022)
Article
Agriculture, Dairy & Animal Science
Margarida Fernandes, Pablo Rodriguez Hernandez, Joao Simoes, Joao Pedro Barbas
Summary: The study revealed that the egg yolk-based semen extender performed better in cryopreserving semen from Merino rams. However, there were significant interactions between different semen extenders, semen processing, and collection months on sperm parameters. Further research is needed to address the causes of these interaction effects and improve semen extenders.
Article
Agriculture, Dairy & Animal Science
Mahdi Nazari, Hossein Daghigh-Kia, Mahdieh Mehdipour, Abouzar Naja
Summary: This study investigated the effects of targeted mitochondrial antioxidant MitoQ and non-targeted antioxidant PTX on the quality of rooster sperm during cooling and cryopreservation. The results showed that the addition of MitoQ and lower concentrations of PTX significantly improved sperm motility and viability, while reducing oxidative stress damage.
Article
Geosciences, Multidisciplinary
Jun Bi, Zhijian Wu, Yu Lu, Haiyan Wen, Yingmin Zhang, Yunxia Shen, Tingting Wei, Guoxu Wang
Summary: The Soil Freezing Characteristic Curve (SFCC) describes the relationship between unfrozen water content and subzero temperature. This study investigated the impacts of initial water content, dry density, soil type, and desalination on SFCC during freezing-thawing process. Results showed that initial water content, soil type, and desalination greatly influenced SFCC, while dry density had little effect. Hysteresis behavior was observed in all soil samples, more obvious in the temperature range of -5°C < T < 0°C.
FRONTIERS IN EARTH SCIENCE
(2023)
Article
Engineering, Environmental
Xu Li, Xiaokang Li
Summary: In this study, a soil freezing-thawing model is proposed based on statistical thermodynamics, which can solve the thermo-hydraulic field problems in the freezing-thawing process. The model derives the phase equilibrium relation for the pore ice-water interface and defines the theoretical relationship between the freezing temperature and the potential energy of pore-water. By using this relationship, the thermo-hydraulic field in the soil freezing-thawing process can be solved. The proposed model is applicable to different types of soil and other porous media undergoing freezing-thawing processes.
COLD REGIONS SCIENCE AND TECHNOLOGY
(2023)
Article
Biology
Anne Hege Alm-Kristiansen
Summary: The cryopreservation of sperm cells is crucial for cattle breeding using artificial insemination. The analysis of sperm motility is important to assess sperm quality after freezing-thawing and before insemination. The study focused on the motile subpopulations of bull spermatozoa before and after cryopreservation and found that the subpopulation of rapid progressive sperm cells was the main indicator of sperm functionality and freezability.
Article
Agriculture, Multidisciplinary
Minhye Kim, Da Woon Jeong, Jun Won Oh, Hyun Jin Jeong, Young Jin Ko, Sung Eun Park, Sung Ok Han
Summary: This study presents a novel strategy for synthesizing L-Ergothioneine (EGT) in Corynebacterium glutamicum, which resulted in increased EGT production. The synthesized EGT exhibited high antioxidant activity, indicating its potential for commercialization.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Bagdat Teltayev, Cesare Oliviero Rossi, Koblanbek Aitbayev, Elena Suppes, Aidos Yelshibayev, Assel Nugmanova
Summary: This paper presents the experimental study of freezing and thawing patterns of highways in Kazakhstan. The study aims to develop a methodology for determining the depth of freezing of subgrade soils and understand the temperature and moisture changes. The results show that temperature fluctuation decreases with depth, and freezing rate varies. The thawing process is relatively uniform but with different rates. The asphalt-concrete layers and upper part of the subgrade experience a frozen state, and moisture changes abruptly at the beginning and end of the cold period.
APPLIED SCIENCES-BASEL
(2022)
Article
Veterinary Sciences
Belen Pequeno, Cristina Castano, Manuel Alvarez-Rodriguez, Paula Boveda, Maria Gemma Millan de la Blanca, Adolfo Toledano-Diaz, Diego Andres Galarza, Heriberto Rodriguez-Martinez, Belen Martinez-Madrid, Julian Santiago-Moreno
Summary: The study aimed to investigate the influence of osmotic changes on the location of aquaporins (AQPs) in spermatozoa during freeze-thawing process. Individual variability in terms of freezeability was observed among rams and their ejaculates. AQP3 expression in the mid- and principal piece of spermatozoa was found to be higher in frozen-thawed samples compared to fresh specimens in the good freezability group, but such differences were not detected in the poor freezability group. AQP3 expression is associated with sperm cryotolerance and can be used as a biomarker.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Food Science & Technology
Tobi Fadiji, Seyed-Hassan Miraei Ashtiani, Daniel Onwude, Zhiguo Li, Umezuruike Linus Opara
Summary: Freezing of food products is a complex process involving heat and mass transfer, which can affect the quality of the frozen product. Numerical modeling, particularly Finite Element Modeling (FEM), is a widely applied tool in the food industry to monitor and control quality changes during freezing and thawing processes. This technique provides accurate predictions and visual information for improved quality control and advancements in cold storage and transport technologies.
Article
Construction & Building Technology
Youneng Liu, Antonin Fabbri, Henry K. K. Wong, Lassana Bakary Traore, Xin Li, Benoit Pardoen
Summary: This paper investigates the damage mechanism of compacted earthen materials subjected to freezing-thawing cycles. The study finds that with an increase in freezing-thawing cycles, the Young's modulus of the material increases in the frozen state and decreases in the thawed state. This can be attributed to the increase in macro-porosity, accumulation of residual deformation, and higher freezing and thawing temperatures. The applied confining pressure provides resistance against permanent deformation.
CONSTRUCTION AND BUILDING MATERIALS
(2023)
Article
Ecology
Annelein Meisner, Basten L. Snoek, Joseph Nesme, Elizabeth Dent, Samuel Jacquiod, Aimee T. Classen, Anders Prieme
Summary: Climate change alters frequencies and intensities of soil drying-rewetting and freezing-thawing cycles, impacting soil water availability crucial for microbial activity. Experiments show that drying-rewetting cycles have stronger effects on soil microbial communities and CO2 production than freezing-thawing cycles.
Article
Agriculture, Dairy & Animal Science
Amer Salman, Estela Fernandez-Alegre, Ruben Francisco-Vazquez, Juan C. Dominguez, Manuel Alvarez-Rodriguez, J. Nestor Caamano, Felipe Martinez-Pastor, Ruben Gomez-Martin, Alejandro Fernandez-Fernandez, Hector Arean-Dablanca
Summary: Artificial insemination is critical for breeding in the dairy industry, and using density gradient centrifugation can improve sperm parameters post-thawing. Single-layer centrifugation is quicker and more economical than double-layer centrifugation, and provides better post-thawing results for low-freezability semen from high-merit bulls.
ANIMAL REPRODUCTION SCIENCE
(2023)
Article
Environmental Sciences
Chunyu Tang, Kui Cheng, Bailiang Liu, Markus Antonietti, Fan Yang
Summary: The combination of liquid artificial humic acid (LA-HA) and freezing-thawing events can enhance carbon sequestration in soils and promote the formation of high fertility black soil. LA-HA applications increase persistent carbon reservoirs and accelerate the formation of resistant carbon. Additionally, LA-HA optimizes the community structure of carbon sequestration bacteria and reduces soil carbon loss after thawing.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biology
Charles A. Elder, Jensen S. Smith, Mustafa Almosawi, Ethan Mills, Brett R. Janis, Jonathan A. Kopechek, Willem F. Wolkers, Michael A. Menze
Summary: By using a biomimetics approach and trehalose as a cryoprotective agent, red blood cells can be successfully preserved for long-term storage without the need for time-consuming washing steps.