Article
Chemistry, Multidisciplinary
Kumar Saurav, Alessia Caso, Petra Urajova, Pavel Hrouzek, Germana Esposito, Katerina Delawska, Marketa Macho, Jan Hajek, Jose Cheel, Subhasish Saha, Petra Divoka, Sila Arsin, Kaarina Sivonen, David P. Fewer, Valeria Costantino
Summary: PUW and MIN are structurally homologous cyclic lipopeptides with antifungal and cytotoxic activities, where the variation in fatty acid substitutions influences the cytotoxicity of the compounds. Different substitutions on the fatty acid moiety of PUW/MIN variants lead to varying degrees of cytotoxicity. A 63 kb puwainaphycin biosynthetic gene cluster was identified in the N. harveyana strain UHCC-0300, indicating the presence of specific lipoinitiation mechanisms and enzymes for modifying the fatty acid moiety.
Article
Plant Sciences
Joon Seong Park, Gwang Rok Ryu, Beom Ryong Kang
Summary: Bacillus subtilis KB21 was found to inhibit the growth and reproduction of plant pathogenic fungus Colletotrichum acutatum by producing antifungal lipopeptides, suggesting its potential as a biological control agent.
Article
Microbiology
Yuka Munakata, Egon Heuson, Theo Daboudet, Barbara Deracinois, Matthieu Duban, Alain Hehn, Francois Coutte, Sophie Slezack-Deschaumes
Summary: The study found that endophytic bacteria affiliated with Bacillus, Janthinobacterium, Yokenella, Enterobacter, Pseudomonas, Serratia, and Microbacterium have antifungal and antibacterial activities, and can produce lipopeptides which have potential pharmaceutical and agricultural value.
Article
Biochemistry & Molecular Biology
Edgar Guevara-Avendano, Mayra Lizette Perez-Molina, Juan Luis Monribot-Villanueva, Elvis Marian Cortazar-Murillo, Monica Ramirez-Vazquez, Frederique Reverchon, Jose Antonio Guerrero-Analco
Summary: Antifungal metabolites produced by avocado rhizobacteria were found to have inhibitory effects on the causal agent of Fusarium dieback. Primary fractions with high inhibitory activity were isolated through bioassay-guided fractionation. The presence of cyclic lipopeptides in the extracts and fractions was confirmed, and the treated fungus showed deformations. These findings highlight the potential of natural products from Bacillus in controlling phytopathogenic fungi.
CHEMISTRY & BIODIVERSITY
(2022)
Article
Biochemistry & Molecular Biology
Damian Neubauer, Maciej Jaskiewicz, Marta Bauer, Agata Olejniczak-Keder, Emilia Sikorska, Karol Sikora, Wojciech Kamysz
Summary: This study found that adding branches fatty acid chains and N-terminal hydrophobic amino acid residues in gemini cationic surfactants can enhance their selectivity towards pathogens, with relatively low hemolytic and cytotoxic activities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Paiboon Tunsagool, Sekkarin Ploypetch, Janthima Jaresitthikunchai, Sittiruk Roytrakul, Kiattawee Choowongkomon, Jatuporn Rattanasrisomporn
Summary: The study demonstrated that cyclic lipopeptides obtained from Bacillus subtilis showed significant antifungal activity against M. canis, a zoonotic pathogen causing ringworms in cats. This suggests an alternative approach for controlling M. canis growth using substances derived from B. subtilis as a biomedicine with antifungal properties.
Article
Biochemistry & Molecular Biology
Paulina Czechowicz, Joanna Nowicka, Damian Neubauer, Grzegorz Chodaczek, Pawel Krzyzek, Grazyna Gosciniak
Summary: In recent years, there has been a growing interest in fungal infections, including vulvovaginal candidiasis (VVC). However, the etiology of this infection remains unexplained, and the role of asymptomatic vaginal Candida colonization is still not clear. Standard methods of determining drug susceptibility may not accurately predict clinical effectiveness, leading to treatment failures and relapse rates. This study aimed to verify the previous in vitro results using a newly developed ex vivo model and to determine the effectiveness of selected ultrashort cyclic lipopeptides (USCLs) and their combinations with fluconazole against Candida biofilms on the vaginal epithelium. The results showed that the tested substances were ineffective in eradicating biofilms ex vivo, but combination therapy showed promising results. The compatibility between different testing methods needs further investigation to improve the understanding and treatment of VVC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Wan-Qi Qin, Dan Fei, Lei Zhou, Yu-Jia Guo, Shun An, Ou-Hang Gong, Yun-Yang Wu, Jin-Feng Liu, Shi-Zhong Yang, Bo-Zhong Mu
Summary: A new member of the surfactin family, surfactin-C-17, has been isolated from Bacillus subtilis TD7 and its primary chemical structure has been determined. Surfatin-C-17 showed a very low CMC and surface tension, as well as strong antibacterial and hemolytic activities. This study suggests that increasing the hydrophobic chain length of surfactin can lead to improved surfactant properties with potential applications in various industries.
NEW JOURNAL OF CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Danielle Santos-Lima, Cristina de Castro Spadari, Vinicius de Morais Barroso, Juliana C. S. Carvalho, Larissa Costa de Almeida, Felipe Santiago Chambergo Alcalde, Marcelo Jose Pena Ferreira, Miriam Sannomiya, Kelly Ishida
Summary: This study isolated and identified a lipopeptide-producing bacterium from a bacterial strain, which showed significant antifungal effects and potential for pharmaceutical development. The lipopeptides exhibited excellent performance in inhibiting fungal growth, disrupting fungal biofilms, and inducing morphological changes, making them promising antifungal agents.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Stephen Olusanmi Akintayo, Behnoush Hosseini, Maliheh Vahidinasab, Marc Messmer, Jens Pfannstiel, Ute Bertsche, Philipp Hubel, Marius Henkel, Rudolf Hausmann, Ralf T. Voegele, Lars Lilge
Summary: This study evaluated the biocontrol ability of two new B. velezensis strains, ES1-02 and EFSO2-04, against fungal phytopathogens Diaporthe spp. and compared with reference strains QST713 and FZB42. The results showed that all strains were effective against the plant pathogens, with the new strains showing comparable antifungal activity to QST713 and slightly lower activity than FZB42.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Nutrition & Dietetics
FuTian Yu, YuanYuan Shen, YaLi Qin, YiYang Pang, HeLiang Fan, JingJing Peng, XiaoDong Pei, XiaoLing Liu
Summary: This study suggests that the lipopeptide extracts (YA215LE) from Bacillus velezensis YA215 isolated from the mangrove area in Beibu Gulf, Guangxi, China, exhibit wide spectrum antibacterial and antifungal activities, with BVYA1 showing significant antibacterial activity and potential as a bactericide.
FRONTIERS IN NUTRITION
(2022)
Article
Microbiology
B. N. Harish, S. N. Nagesha, B. N. Ramesh, S. Shyamalamma, M. S. Nagaraj, H. C. Girish, C. Pradeep, K. S. Shiva Kumar, K. S. Tharun Kumar, S. N. Pavan, V. Kavan Kumar
Summary: This study investigated the antifungal activities of lipopeptides from different B. subtilis strains against Alternaria alternata, and found that the T6 strain showed the highest antifungal activity with a suppression rate of 85.88%.
Article
Food Science & Technology
Shengjie Li, Xingang Xu, Tianyuan Zhao, Jianing Ma, Luning Zhao, Qi Song, Weihong Sun
Summary: This study isolated an antagonist strain of Bacillus velezensis with obvious antifungal activity against A. flavus from the surface of healthy rice. The lipopeptides (LPs) produced by this strain could inhibit spore germination and hyphal growth of A. flavus, and down-regulated key genes involved in biosynthesis pathways.
Article
Biotechnology & Applied Microbiology
Zhen Li, Kleinberg X. Fernandez, John C. Vederas, Michael G. Ganzle
Summary: This study aims to investigate the interaction between Bacillus species and fungi in daqu models. It was found that B. velezensis FUA2155 displayed strong antifungal activity in the daqu model and was the only strain that produced multiple iturin A congeners in situ.
Article
Biochemistry & Molecular Biology
Fei-Hua Yao, Xiao Liang, Xia Cheng, Juan Ling, Jun-De Dong, Shu-Hua Qi
Summary: Three new cyclic lipopeptides and four new linear peptides were isolated from a marine fungus, showing significant antifungal activity. The beta-amino fatty acid chain was found to significantly affect the antifungal activity of the cyclic lipopeptides.
Article
Biochemistry & Molecular Biology
Daniel Tello, Laura Natalia Gonzalez-Garcia, Jorge Gomez, Juan Camilo Zuluaga-Monares, Rogelio Garcia, Ricardo Angel, Daniel Mahecha, Erick Duarte, Maria del Rosario Leon, Fernando Reyes, Camilo Escobar-Velasquez, Mario Linares-Vasquez, Nicolas Cardozo, Jorge Duitama
Summary: The article introduces the latest functionalities implemented in NGSEP 4 for identifying orthogroups and conducting whole genome alignments. NGSEP 4 also includes a new graphical user interface based on JavaFX technology. These new developments are expected to be very useful for studies in evolutionary biology and population genomics.
MOLECULAR ECOLOGY RESOURCES
(2023)
Article
Microbiology
Giuliano Gattoni, Rafael R. de la Haba, Jesus Martin, Fernando Reyes, Cristina Sanchez-Porro, Antonia Feola, Candida Zuchegna, Shaday Guerrero-Flores, Mario Varcamonti, Ezio Ricca, Nelly Selem-Mojica, Antonio Ventosa, Paulina Corral
Summary: The low-abundance microbiota in the marine environment has the potential to produce new bioactive molecules with pharmaceutical applications. This study isolated a new marine bacterium, Leeuwenhoekiella parthenopeia sp. nov. Mr9(T), from the rare biosphere using seasonal and diel sampling approaches. The bacterium showed potential for synthesizing novel bioactive compounds, and its lipidome exhibited activity against prostatic and brain tumor cells. The study emphasizes the importance of ecological sampling strategies and the use of bacterial membrane constituents in developing novel therapeutics.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Frederick Boye Annang, Guiomar Perez-Moreno, Cristina Bosch-Navarrete, Victor Gonzalez-Menendez, Jesus Martin, Thomas A. A. Mackenzie, Maria C. C. Ramos, Luis M. M. Ruiz-Perez, Olga Genilloud, Dolores Gonzalez-Pacanowska, Francisca Vicente, Fernando Reyes
Summary: Memnoniella is a fungal genus that produces a variety of biologically active compounds. A specific extract from Memnoniella dichroa CF-080171 showed potent activity against Plasmodium falciparum 3D7 and Trypanosoma cruzi Tulahuen parasites. Eight new meroterpenoids with varying potencies were isolated from the extract, and compounds 1, 2, and 4 exhibited strong antiparasitic activity against the mentioned parasites and cytotoxic activity against HepG2 cells.
Article
Microbiology
Marina Sanchez-Hidalgo, Maria Jesus Garcia, Ignacio Gonzalez, Daniel Oves-Costales, Olga Genilloud
Summary: In this study, a strain of Kribbella sp. CA-293567 was found to produce sandramycin and kribbellichelins A-B, which are new secondary metabolites. The complete genome sequencing and in silico identification of biosynthetic gene clusters were performed, focusing on the pathways encoding these compounds. A comparative analysis of the biosynthetic potential of 38 publicly available genomes from Kribbella strains was also presented.
Article
Biochemistry & Molecular Biology
Suhui Ye, Brian Molloy, Ignacio Perez-Victoria, Ignacio Montero, Alfredo F. Brana, Carlos Olano, Sonia Arca, Jesus Martin, Fernando Reyes, Jose A. Salas, Carmen Mendez
Summary: Genome mining using bioinformatics tools allowed for uncovering hidden biosynthesis gene clusters, while an alternative approach involving the analysis of Streptomyces Antibiotic Regulatory Proteins (SARP) encoding genes was used to identify cryptic gene clusters. This strategy led to the discovery of the new ahb cluster in Streptomyces argillaceus and the identification of ahbamycins as a new family of metabolites with antibiotic and antitumor activity. Further investigation revealed a hybrid compound, AHB18, produced through metabolic crosstalk between the ahb and argimycin P pathways. This finding opens up possibilities for generating new compounds through combinatorial biosynthesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Medicinal
Susana P. Gaudencio, Engin Bayram, Lada Lukic Bilela, Mercedes Cueto, Ana R. Diaz-Marrero, Berat Z. Haznedaroglu, Carlos Jimenez, Manolis Mandalakis, Florbela Pereira, Fernando Reyes, Deniz Tasdemir
Summary: Natural Products (NP) play a crucial role in the discovery of novel drugs and biotechnological applications. However, the process of NP discovery is expensive and time-consuming, mainly due to the challenges of dereplication and structure elucidation, especially for metabolites with stereogenic centers. This review highlights recent technological and instrumental advances that address these obstacles and accelerate NP discovery for biotechnological applications. It emphasizes innovative high-throughput tools and methods for bioactivity screening, NP chemical analysis, dereplication, metabolite profiling, metabolomics, genome sequencing and/or genomics approaches, databases, bioinformatics, chemoinformatics, and three-dimensional NP structure elucidation.
Article
Chemistry, Organic
Francisco Javier Ortiz-Lopez, Daniel Oves-Costales, Daniel Carretero-Molina, Jesus Martin, Caridad Diaz, Mercedes de la Cruz, Fernando Roman-Hurtado, Maria Alvarez-Arevalo, Tue Sparholt Jorgensen, Fernando Reyes, Tilmann Weber, Olga Genilloud
Summary: Crosiellidines are intriguing pyrazine-alkylguanidine metabolites isolated from the minor actinomycete genus Crossiella. They possess a pyrazine scaffold and uncommon guanidine prenylations, including an exotic O-prenylated N-hydroxyguanidine moiety. The novel substitution pattern of the 2-methoxypyrazine core inaugurates a new class of naturally occurring pyrazine compounds, with potential biosynthetic implications. Isotopic feeding and genome analysis have led to the proposal of a biosynthetic pathway from arginine. The crossiellidines exhibit remarkable, broad-spectrum antibacterial activity.
Article
Multidisciplinary Sciences
Julia P. Deisinger, Melina Arts, Ioli Kotsogianni, Jan-Samuel Puls, Fabian Grein, Francisco Javier Ortiz-Lopez, Nathaniel I. Martin, Anna Mueller, Olga Genilloud, Tanja Schneider
Summary: Antibiotic resistance is a major concern, and new antibiotics with novel chemistry and mechanisms of action are needed. The recently discovered antibiotic cacaoidin is the first class V lanthipeptide, combining unique structural features and D-amino acid content. It shows antimicrobial activity against gram-positive pathogens and interferes with peptidoglycan biosynthesis. Further studies have demonstrated that cacaoidin exhibits a dual mode of action by binding to lipid IIPGN and inhibiting cell wall transglycosylases.
Article
Biotechnology & Applied Microbiology
Daniel Oves-Costales, Tetiana Gren, Eva Baggesgaard Sterndorff, Jesus Martin, Francisco Javier Ortiz-Lopez, Tue S. Jorgensen, Xinglin Jiang, Fernando Roman-Hurtado, Fernando Reyes, Olga Genilloud, Tilmann Weber
Summary: This study successfully identified the biosynthetic gene cluster of Globomycin using genome mining, and demonstrated the close correlation between the gene cluster and Globomycin production. This discovery paves the way for the synthesis of novel Globomycin derivatives with improved pharmacological properties.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ramon I. Santamaria, Ana Martinez-Carrasco, Jose R. Tormo, Jesus Martin, Olga Genilloud, Fernando Reyes, Margarita Diaz
Summary: The co-culturing of microorganisms is a well-known strategy to study microbial interactions in the laboratory. In this study, the effects of the interaction between nine Streptomyces species and predator bacteria Myxococcus xanthus were investigated. It was found that certain Streptomyces species induce mound formation of M. xanthus on complex media. Moreover, the study identified siderophores produced by S. griseus and molecules produced by S. peucetius that affect the behavior of M. xanthus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Jose Diogo Neves dos Santos, Dominika Klimek, Magdalena Calusinska, Alexandre Lobo-da-Cunha, Jose Catita, Hugo Goncalves, Ignacio Gonzalez, Fernando Reyes, Olga Maria Lage
Summary: A novel actinomycetal strain, designated MTZ3.1T, was isolated from the Tagus estuary in Alcochete, Portugal, and subjected to a polyphasic taxonomic study. Based on the morphology and phylogeny, MTZ3.1T was identified as a new species, Streptomyces meridianus. It showed unique characteristics in terms of growth conditions, fatty acid composition, and antimicrobial activity against Staphylococcus aureus.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Wei Huang, Janneth Rodrigues, Etienne Bilgo, Jose R. Tormo, Joseph D. Challenger, Cristina De Cozar-Gallardo, Ignacio Perez-Victoria, Fernando Reyes, Pablo Castaneda-Casado, Edounou Jacques Gnambani, Domonbabele Francois de Sales Hien, Maurice Konkobo, Beatriz Urones, Isabelle Coppens, Alfonso Mendoza-Losana, Lluis Ballell, Abdoulaye Diabate, Thomas S. Churcher, Marcelo Jacobs-Lorena
Summary: The article describes the properties of a symbiotic bacterium, Delftia tsuruhatensis TC1, which inhibits early stages of Plasmodium development and transmission. The bacterium secretes a small-molecule inhibitor called harmane, which penetrates the mosquito's cuticle and inhibits Plasmodium development. Field studies in Burkina Faso and modeling show that D. tsuruhatensis TC1 has the potential to complement mosquito-targeted malaria transmission control.
Article
Microbiology
Rachel Serrano, Victor Gonzalez-Menendez, Jose R. Tormo, Olga Genilloud
Summary: This paper aims to develop and validate a high-throughput screening platform for evaluating the antifungal potential of chemicals and natural products against four important plant pathogens. The optimal assay conditions were determined through several experiments, and the platform was validated using known antifungal agents and microbial natural product extracts.