Article
Immunology
Yoonsung Hu, Nogi Park, Keun Seok Seo, Joo Youn Park, Radha P. Somarathne, Alicia K. Olivier, Nicholas C. Fitzkee, Justin A. Thornton
Summary: The study reveals that pneumococcal surface adhesin A interacts with host cellular receptor ANXA2, indicating the importance of ANXA2 in pneumococcal colonization.
Article
Immunology
Mengze Guo, Xiaonan Guo, Chenxing Zhang, Shidong Zhu, Yue Zhang, Tiejun Gu, Wei Kong, Yongge Wu
Summary: Pneumococcal disease is a serious global public health problem. Vaccination is an effective method to prevent and control the disease. A protein-polysaccharide conjugate vaccine was developed using biotin and streptavidin. The vaccine showed high efficacy in producing antibodies, inducing immune responses, and providing protection against the pathogen in mice.
INFECTION AND IMMUNITY
(2022)
Article
Biochemical Research Methods
Xiaonan Guo, Qing Sun, Hualong Xi, Yue Zhang, Mengze Guo, Chenxing Zhang, Shidong Zhu, Tiejun Gu, Wei Kong, Yongge Wu
Summary: This study demonstrated the successful expression and purification of the PsaA-PspA fusion protein using hydroxyapatite and two-step chromatography. The purified protein induced high levels of antibodies in mice after immunization. The results suggest that this purification method is effective in producing high-purity PsaA-PspA fusion protein with biological activity and chemical integrity.
PROTEIN EXPRESSION AND PURIFICATION
(2021)
Review
Immunology
Sha Li, Hangeri Liang, Shui-Hao Zhao, Xiao-Yan Yang, Zhong Guo
Summary: This review provides an overview of the advancements made in the development of protein-based pneumococcal vaccines. The key protein vaccine candidates and their vaccination results in animal studies are discussed, as well as the challenges and future directions in protein-based pneumococcal vaccine.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Rong Huang, Haichao Feng, Zhihui Xu, Nan Zhang, Yunpeng Liu, Jiahui Shao, Qirong Shen, Ruifu Zhang
Summary: This study investigates the adhesion of PGPR strain Bacillus velezensis SQR9 to both plant root surfaces and abiotic surfaces. The results show that adhesion is necessary for root colonization and that FliD and YhaN proteins play vital roles in root adhesion.
MOLECULAR PLANT-MICROBE INTERACTIONS
(2022)
Review
Microbiology
Ronnie G. Willaert, Yeseren Kayacan, Bart Devreese
Summary: This article discusses the adhesion of fungal pathogens to host tissue cells or abiotic surfaces during infections, with a focus on the roles of the Flo adhesin family. The authors reviewed the domain architectures, functions, and classifications of Flo adhesins, as well as detailed discussions on the structure, function, and adhesion mechanisms of those with solved structures. They also identified yeasts expressing Flo adhesins from the Pfam database and discussed their adhesion characteristics and involvement in infections.
Article
Biochemical Research Methods
Maruthi Prasanna, Ruben Varela Calvino, Annie Lambert, Maria Arista Romero, Sylvia Pujals, Francois Trottein, Emilie Camberlein, Cyrille Grandjean, Noemi Csaba
Summary: This study demonstrates the potential of chitosan-based nanovaccines for efficient delivery of glycoconjugate antigens. Encapsulated glycoconjugates showed enhanced uptake by cells and better immunostimulatory properties compared to naked antigens. The study also showed a 100-fold increase in IgG response with the encapsulated glycoconjugate compared to the nonencapsulated group in mice.
BIOCONJUGATE CHEMISTRY
(2023)
Article
Biochemical Research Methods
Maruthi Prasanna, Ruben Varela Calvino, Annie Lambert, Maria Arista Romero, Sylvia Pujals, Francois Trottein, Emilie Camberlein, Cyrille Grandjean, Noemi Csaba
Summary: This study demonstrates the potential of chitosan-based nanovaccine for efficient delivery of glycoconjugate antigens. The encapsulation of the glycoconjugate using chitosan nanoparticles (CNPs) enhanced its immunogenicity and resulted in a significantly higher immune response compared to the nonencapsulated glycoconjugate. These findings suggest that CNPs could be a promising delivery system for pneumococcal conjugate vaccines.
BIOCONJUGATE CHEMISTRY
(2023)
Review
Immunology
Ayumi Morimura, Shigeto Hamaguchi, Yukihiro Akeda, Kazunori Tomono
Summary: Research on Streptococcus pneumoniae has shown that it is a commensal in the human upper respiratory tract as well as a pathogen causing various diseases. The lack of tractable animal models has hindered the full understanding of its transmission mechanism, but recent studies and novel animal models have helped shed light on this aspect.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Immunology
Yang Liu, Bowen Li, Yunwei Wei
Summary: This review focuses on the role of gut microbiota in the occurrence of anastomotic leak (AL) following colorectal surgery. It discusses the changes in gut microbiota profiles in patients with AL, the colonization and tissue breakdown effects of AL-specific pathogenic or collagenase bacteria at the anastomosis site, and the potential mechanisms of pathogen-induced impaired anastomotic healing.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Review
Pharmacology & Pharmacy
Claudine El-Beyrouty, Rebecca Buckler, Meghan Mitchell, Samantha Phillips, Sara Groome
Summary: Streptococcus pneumoniae remains a significant pathogen causing both mild and severe infections, and the development of vaccines and improved vaccination rates have greatly reduced mortality.
Article
Multidisciplinary Sciences
Maria Dolores Cima Cabal, Felipe Molina, Jose Ignacio Lopez-Sanchez, Efren Perez-Santin, Maria del Mar Garcia-Suarez
Summary: This systematic review evaluates pneumolysin (PLY) as a target for new treatments against pneumococcal infections. Forty-one articles were reviewed, and molecules that inhibit PLY were classified into three main groups: those exerting a direct effect, those acting indirectly, and those with unknown mechanisms. Some molecules have the potential for clinical implementation, while others require further research. These findings may contribute to the improvement of clinical management for patients with pneumococcal infections.
Review
Anesthesiology
Michael C. Ferraro, R. Andrew Moore, Amanda C. de C. Williams, Emma Fisher, Gavin Stewart, McKenzie C. Ferguson, Christopher Eccleston, Neil E. O'Connell
Summary: This study aimed to explore the extent and characteristics of retracted publications in pain research. The results showed a significant increase in the number of retracted pain articles over time, with the majority of retractions being due to misconduct. The median time from article publication to retraction was 2 years, and the time differed depending on the reason for retraction. Further investigations into the fate of retracted pain articles are necessary to determine the impact of unreliable data on pain research.
Review
Immunology
Kay Choong See
Summary: Pneumococcal disease is still a major cause of severe illness in both children and adults. Vaccines are available to prevent severe disease, but guidelines for pneumococcal vaccination in adults are limited and do not consider individual patients. This review identifies considerations for individualized decision-making, such as risk of severe disease, immunogenicity, efficacy, and other factors.
Article
Multidisciplinary Sciences
Fatemeh Ramezanalizadeh, Iraj Rasooli, Parviz Owlia, Shakiba Darvish Alipour Astaneh, Raziyeh Abdolhamidi
Summary: In this study, a combined vaccine using planktonic and biofilm antigens of Acinetobacter baumannii was evaluated for its immune response and protective efficacy against lethal doses of two Carbapenem-resistant high adherent A. baumannii strains in a murine sepsis model. The results showed that the combined vaccine elicited high antibody titers and achieved complete bacterial clearance in affected tissues, highlighting the importance of considering both planktonic and biofilm antigens in vaccine design.
SCIENTIFIC REPORTS
(2022)