Article
Biochemistry & Molecular Biology
Yongbo Yang, Xiaoying Huang, Wanli Yuan, Yang Xiang, Xueqin Guo, Wei Wei, Mario Soberon, Alejandra Bravo, Kaiyu Liu
Summary: The study found that Cry toxins induce autophagy only in susceptible cell lines, depending on AMPK and JNK pathways. Inhibiting autophagy delays cell death triggered by Cry toxins.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Environmental Sciences
Nathalia Nocchi, Alejandro Gonzalez-Orive, Alberto Hernandez-Creus, Jacob Lorenzo-Morales, Adriana Rodriguez, Rodrigo Morchon, Ana R. Diaz-Marrero, Jose J. Fernandez
Summary: Millepora alcicornis, also known as fire coral, is a tropical hydrozoa species that produces thermolabile proteins, including phospholipase A2 (PLA2) and alciporin, a pore-forming protein (PFP). The venom contained in the nematocysts of this species displays hemolytic activity primarily through the action of alciporin, which targets sphingomyelin. Atomic force microscopy (AFM) was used to study the evolution of hemolytic damage at the nanoscale.
FRONTIERS IN MARINE SCIENCE
(2022)
Review
Food Science & Technology
Yajuan Li, Yuelong Li, Hylemariam Mihiretie Mengist, Cuixiao Shi, Caiying Zhang, Bo Wang, Tingting Li, Ying Huang, Yuanhong Xu, Tengchuan Jin
Summary: Pore-forming toxins (PFTs) are a major virulence factor of many pathogenic bacteria, with well-characterized structural basis and interactions with host cell membranes. Understanding the mechanisms of PFTs can contribute to the development of novel therapeutic strategies and combat bacterial antibiotic resistance.
Article
Food Science & Technology
Tipaporn Kumkoon, Chalongrat Noree, Panadda Boonserm
Summary: Breast cancer is a common cancer in women, and conventional chemotherapy has adverse side effects on healthy tissues. Therefore, combining pore-forming toxins with cell-targeting peptides is a promising strategy for selectively destroying cancer cells. In this study, a luteinizing hormone-releasing hormone (LHRH) peptide was fused to the BinB toxin to target MCF-7 breast cancer cells specifically. The results showed that LHRH-BinB inhibited MCF-7 cell proliferation while leaving normal cells unaffected, suggesting its potential as a cancer therapeutic agent.
Article
Microbiology
Mahendra Singh, N. Rupesh, Shashi Bhushan Pandit, Kausik Chattopadhyay
Summary: In this study, it was found that curcumin can neutralize the membrane-damaging activity of VCC, making it a potential inhibitor of this powerful bacterial toxin. Curcumin binds to VCC and reduces its ability to attack cell membranes, while the soluble extract of curcumin generated by heat treatment weakens the pore-forming activity of VCC.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Katharina Ernst, Marc Landenberger, Julian Nieland, Katharina Norgaard, Manfred Frick, Giorgio Fois, Roland Benz, Holger Barth
Summary: The study investigated the cytotoxic effect of CDTb on cells, revealing that CDTb impairs cell viability and leads to redistribution of F-actin without affecting tubulin structures in CaCo-2 cells. Pharmacological pore blockers were found to effectively inhibit the cytotoxicity induced by pore formation of CDTb in the cytoplasmic membrane.
Article
Biochemistry & Molecular Biology
Anish Kumar Mondal, Kusum Lata, Mahendra Singh, Shamaita Chatterjee, Aakanksha Chauhan, Sindhoora Puravankara, Kausik Chattopadhyay
Summary: This article discusses the mechanism of pore formation by pore-forming toxins (PFTs), with a particular focus on the mechanistic details contributed by cryo-EM-based structural studies.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2022)
Article
Biochemistry & Molecular Biology
Deepinder Kaur, Pratima Verma, Mahendra Singh, Arpita Sharma, Kusum Lata, Arunika Mukhopadhaya, Kausik Chattopadhyay
Summary: This study reveals that VCC can induce apoptotic cell death and cytotoxicity, even without its pore-forming ability. These findings have significant implications for understanding the pathogenicity and functionality of VCC.
Article
Food Science & Technology
Yu Larpin, Herve Besancon, Victoriia S. Babiychuk, Eduard B. Babiychuk, Rene Koffel
Summary: The research findings demonstrate that small pore-forming toxins, such as lysenin and aerolysin, have different effects on cells, with lysenin causing calcium influx and activating membrane repair mechanisms, while aerolysin does not. Cell resistance is influenced by factors such as binding efficiency, pore localization, and the activation of membrane repair mechanisms.
Article
Biochemistry & Molecular Biology
Jing Huang, Baohua Zhao, Tingting Liu, Lin Kang, Jiaxin Li, Zishuo Guo, Ming Chen, Shan Gao, Jing Wang, Yanwei Li, Jinglin Wang, Wenwen Xin
Summary: This study found that Zaragozic acid (ZA) significantly reduced the toxicity of Epsilon toxin (ETX) in cells. ZA did not affect the binding of ETX to cells, but disrupted the ability of ETX to form pores or oligomers, reducing its harm to cells. Additionally, ZA decreased phosphatidylserine exposure and increased calcium influx in cells. ZA also protected mice from ETX in vivo, suggesting its potential as a broad-spectrum medicine for multiple toxin-induced diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jaume Torres, Wahyu Surya, Panadda Boonserm
Summary: This study used Alphafold-2 to predict the structure of Bt toxins and proposed possible oligomeric intermediates in the membrane insertion process. The research provides important clues for revealing the mechanism of action of Bt toxins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Wei Yuen Yap, Jung Shan Hwang
Summary: HALT-4 differs from other actinoporins due to its N-terminal propart containing additional residues. Five truncated versions of HALT-4 were created to investigate the role of the N-terminal region and potential cleavage sites. Results showed that proHALT-4, tKK1, and tKK2 exhibited similar cytolytic activity, while tRK3, tKK4, and tKK5 failed to kill cells, suggesting cleavage did not enhance cytolytic activity but may aid in sorting to secretory pathway.
Article
Food Science & Technology
Vincent Deruelle, Alice Berry, Stephanie Bouillot, Viviana Job, Antoine P. Maillard, Sylvie Elsen, Philippe Huber
Summary: ExlA is a highly virulent pore-forming toxin secreted by outlier strains of Pseudomonas aeruginosa, accumulating at specific points of the outer membrane when intracellular levels of c-di-GMP are high. Research shows that ExlA is poorly secreted in liquid culture, but highly detectable in mice infected with an exlA+ strain.
Article
Environmental Sciences
Zdenka Svobodova, Oxana Skokova Habustova, Hana Sehadova
Summary: The uptake and potential intergenerational transfer of Cry1Ab insecticide from genetically engineered maize to the predator Harmonia axyridis were investigated. Cry1Ab concentration was significantly lower in the herbivore Rhopalosiphum padi and the predator H. axyridis compared to GE maize. Cry1Ab did not affect the performance or cause any pathological changes in H. axyridis, suggesting that the detected route of exposure is unlikely to impact the biological control of this predator in agroecosystems.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Pratima Verma, Kausik Chattopadhyay
Summary: Thermostable direct hemolysin (TDH) is the major virulence determinant of the gastroenteric bacterial pathogen Vibrio parahaemolyticus. TDH is a unique tetrameric pore-forming toxin with structural similarity to eukaryotic PFTs produced by sea anemones. The specific membrane pore structure and interaction mechanisms of TDH remain unclear.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Review
Plant Sciences
Amanda O. Rodrigues, Louise L. May L. De Mio, Carlos R. Soccol
Summary: Molecular studies have revealed Trichoderma's biocontrol mechanisms, making it an alternative to pesticides for disease control. Trichoderma-treated plants benefit from disease control and growth promotion through gene expression modulation. The commercialization and industrialization of Trichoderma technologies are increasing worldwide.
Article
Food Science & Technology
Alexander da Silva Vale, Gabriel Balla, Luiz Roberto Saldanha Rodrigues, Dao Pedro de Carvalho Neto, Carlos Ricardo Soccol, Gilberto Vinicius de Melo Pereira
Summary: This study investigated the microbial community structure and chemical changes in different layers of a static coffee beans fermentation tank over different time periods (24, 48, and 72 h). The results showed that extending the fermentation time resulted in increased production of key metabolites and diversification of sensory profiles of coffee beverages.
Article
Agricultural Engineering
Walter J. Martinez-Burgos, Luciana Porto de Souza Vandenberghe, Susan Grace Karp, Ariane Fatima Murawski de Mello, Vanete Thomaz Soccol, Carlos Ricardo Soccol
Summary: Soybean hulls, a lignocellulosic residue from soybean processing, can be valorized as a carbon source for biolipid production. This study optimized the process for producing lipids from soybean hulls using Lipomyces starkeyi LPB 53 strain. The maximum cell biomass concentration reached was 26.5 g/L with 42.5% lipids, and a significant portion of xylose was consumed. The obtained oil was mainly composed of oleic, palmitic, palmitoleic, linoleic, and stearic fatty acids.
BIORESOURCE TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Beatriz A. Braz, Manuel Hospinal-Santiani, Gustavo Martins, Jeferson L. Gogola, Marcia G. P. Valenga, Breno C. B. Beirao, Marcio F. Bergamini, Luiz H. Marcolino-Junior, Vanete Thomaz-Soccol, Carlos R. Soccol
Summary: Electrochemical immunosensors with synthetic peptides and nanomaterials such as gold nanoparticles have been used to detect SARS-CoV-2 Anti-S antibodies. A solid-binding peptide was used as the recognition site, capable of recognizing antibodies of the spike protein. The immunosensor showed good selectivity and sensitivity for antibody detection in human serum samples.
Article
Green & Sustainable Science & Technology
Diego Alejandro Nieto-Monteros, Rafaela de Oliveira Penha, Carlos Ricardo Soccol
Summary: Plants can only absorb a limited amount of nutrients from the soil, so foliar fertilization using sulfuric bone meal hydrolysate has been proven to be a good alternative. In this study, the effects of foliar application of neutralized sulfuric bone meal hydrolysate on soybean growth parameters and nutrient concentrations were evaluated. Results showed that soybean plants treated with 1% NSBMH had significantly improved growth and nutrient concentrations compared to the control group. Therefore, recycling cattle bone waste to obtain alternative phosphorus through neutralized sulfuric bone meal hydrolysate is a sustainable and environmentally-friendly option.
Review
Biotechnology & Applied Microbiology
Julio Cesar de Carvalho, Luciana Porto de Souza Vandenberghe, Eduardo Bittencourt Sydney, Susan Grace Karp, Antonio Irineudo Magalhaes Jr, Walter Jose Martinez-Burgos, Adriane Bianchi Pedroni Medeiros, Vanete Thomaz-Soccol, Sabrina Vieira, Luiz Alberto Junior Letti, Cristine Rodrigues, Adenise Lorenci Woiciechowski, Carlos Ricardo Soccol
Summary: Sugarcane ethanol production generates a large amount of vinasse, which can be used as a suitable substrate for anaerobic digestion to improve energy output. This review discusses the current technology for ethanol production from sugarcane and describes the state of the art in vinasse anaerobic digestion.
FERMENTATION-BASEL
(2023)
Review
Biotechnology & Applied Microbiology
Alexander da Silva Vale, Gilberto Vinicius de Melo Pereira, Ana Caroline de Oliveira, Dao Pedro de Carvalho Neto, Leonardo Wedderhoff Herrmann, Susan Grace Karp, Vanete Thomaz Soccol, Carlos Ricardo Soccol
Summary: The skin microbiome, consisting of bacteria, fungi, and viruses, plays a critical role in preventing the overgrowth of harmful microorganisms. Probiotic-derived metabolites, known as postbiotics, have emerged as promising bioactive compounds for skin health. Postbiotics, produced by lactic acid bacteria and yeast, possess antimicrobial, immunomodulatory, antioxidant, and anti-inflammatory activities. They also offer advantages in terms of stability and safety compared to probiotics. This article reviews the large-scale production of postbiotics and their applications in cosmetic formulations, as well as discussing their efficacy in treating various skin conditions. Technological advancements in the field of postbiotics are also highlighted through a survey of patents.
FERMENTATION-BASEL
(2023)
Article
Chemistry, Analytical
Beatriz A. Braz, Manuel Hospinal-Santiani, Gustavo Martins, Breno C. B. Beirao, Marcio F. Bergamini, Luiz H. Marcolino-Junior, Carlos R. Soccol, Vanete Thomaz-Soccol
Summary: Neglected tropical diseases, which are endemic in low-income populations, lack research investment and have unacceptable indicators. Electrochemical immunosensors show promise for diagnosis in isolated regions. In this study, different peptides were investigated for detecting Leishmania antibodies with outstanding performance achieved using a graphene affinity peptide as a recognition site.
Review
Food Science & Technology
Kim Kley Valladares-Diestra, Luciana Porto de Souza Vandenberghe, Sabrina Vieira, Luis Daniel Goyzueta-Mamani, Patricia Beatriz Gruening de Mattos, Maria Clara Manzoki, Vanete Thomaz Soccol, Carlos Ricardo Soccol
Summary: Concerns about a good-quality diet have risen in recent years, leading to an increased interest in food supplements like prebiotics that offer great nutritional and health benefits. Xylooligosaccharides (XOs) show high potential among various prebiotics for preventing systemic disorders. While XOs can be found in natural sources, their production is limited. Lignocellulosic biomasses have been identified as a promising source for XOs production, using agro-industrial by-products. However, physicochemical pretreatments are required to obtain XOs, and different methods are discussed to increase their production and limit the production of toxic compounds.
Article
Biotechnology & Applied Microbiology
Priscilla Zwiercheczewski de Oliveira, Luciana Porto de Souza Vandenberghe, Carlos Ricardo Soccol
Summary: This study presents the production of lactic acid from sugarcane juice and explores the use of ion-exchange resins for its purification. The fermentation kinetics showed the highest lactic acid production with a productivity of 1.18 g LA/L center dot h. The combined use of Amberlite IR120 and IRA-67 resins in a stirred tank resulted in a final lactic acid concentration of 189.11 g/L with 95% mass recovery. This simplified approach demonstrates the viability of using ion-exchange resins in a controlled environment and for scale-up processes.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Veronica Sayuri Nishida, Adenise Lorenci Woiciechowski, Kim Kley Valladares-Diestra, Luis Alberto Zevallos Torres, Luciana Porto de Souza Vandenberghe, Arion Zandona Filho, Carlos Ricardo Soccol
Summary: Soybean hulls, which make up 5-8% of the whole grain, are the primary industrial waste from soybean processing. Imidazole was used to hydrothermally pretreat soybean hulls and produce bioethanol. Different pretreatment temperatures and times were tested, and lignin removal and glucose yield were affected by temperature. Enzymatic hydrolysis of imidazole-treated soybean hulls resulted in significant glucose and xylose yields. Fermentation of the enzymatic hydrolysate by Saccharomyces cerevisiae achieved a high bioethanol yield. Imidazole shows potential as an alternative pretreatment method for lignocellulosic wastes like soybean hulls to produce second-generation biofuels and other biomolecules.
FERMENTATION-BASEL
(2023)
Article
Parasitology
Felipe Dutra Rego, Eduardo Sergio da Silva, Valeriana Valadares Lopes, Rafael Goncalves Teixeira-Neto, Vinicius Silva Belo, Antonio Augusto Fonseca Jr, Diego Andrade Pereira, Heber Paulino Pena, Marcia Dalastra Laurenti, Gabriela Araujo, Vania Lucia Ribeiro da Matta, Islam Hussein Chouman, Thaina Bergantin Burrin, Carmen M. Sandoval, Stella Maria Barrouin-Melo, Flaviane Alves de Pinho, Helida Monteiro de Andrade, Ramon Vieira Nunes, Celia Maria Ferreira Gontijo, Vanete Thomaz Soccol, Donnamae Klocek, Danyil Grybchuk, Diego Henrique Macedo, Rubens Lima do Monte-Neto, Vyacheslav Yurchenko, Rodrigo Pedro Soares
Summary: This study is the first to detect the presence of LRV2 in the New World, which is significant for understanding the transmission and impact of this virus.
MEMORIAS DO INSTITUTO OSWALDO CRUZ
(2023)