Article
Food Science & Technology
Kristen Clermont, Charles J. J. Graham, Steven W. W. Lloyd, Casey C. C. Grimm, Jennifer J. J. Randall, Christopher P. P. Mattison
Summary: Proteomic analyses were used to study the protein accumulation during pecan kernel development. Proteins were analyzed through gel-free and label-free mass spectrometry and 2-D gel electrophoresis. Specific proteins related to pecan allergens, histones, and different stages of development were identified. These findings provide a foundation for future research on identifying proteins associated with desirable traits in pecans.
Article
Biochemical Research Methods
Khushman Taunk, Debasish Paul, Raju Dabhi, Chanukuppa Venkatesh, Saikiran Jajula, Venkateshwarlu Naik, Anup Tamhankar, Tufan Naiya, Manas Kumar Santra, Srikanth Rapole
Summary: Proteins play a crucial role in modern biology, and the study of proteomics is important for understanding the protein content in biological samples. This study developed a simple single step method for efficient protein extraction, applicable to a wide range of biological samples.
Article
Biochemical Research Methods
Devon Kohler, Mateusz Staniak, Tsung-Heng Tsai, Ting Huang, Nicholas Shulman, Oliver M. Bernhardt, Brendan X. MacLean, Alexey I. Nesvizhskii, Lukas Reiter, Eduard Sabido, Meena Choi, Olga Vitek
Summary: The MSstats R-Bioconductor family of packages, particularly the new version MSstats v4.0, is widely used for statistical analyses of quantitative proteomic experiments. It provides improved usability, versatility, and accuracy of statistical methodology, as well as better memory use and computation speed. Empirical comparisons have demonstrated the stronger performance and better usability of MSstats v4.0 compared to existing methods.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Plant Sciences
Saroj Kumar Sah, Salah Jumaa, Jiaxu Li, K. Raja Reddy
Summary: This study investigates the proteomic response of rice leaves to UV-B stress and identifies several proteins involved in the adaptation of rice to UV-B stress. The findings provide valuable insights for the development of UV-B stress-resistant rice varieties in the future.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Maria Luisa Castrejon-Godinez, Efrain Tovar-Sanchez, Ma Laura Ortiz-Hernandez, Sergio Encarnacion-Guevara, Angel Gabriel Martinez-Batallar, Magdalena Hernandez-Ortiz, Enrique Sanchez-Salinas, Alexis Rodriguez, Patricia Mussali-Galante
Summary: Methyl parathion, a widely used pesticide, poses a significant threat to ecosystems and human health due to its high toxicity and persistence in the environment. Identifying microorganisms capable of degrading methyl parathion is crucial for bioremediation efforts at pesticide-polluted sites.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Chemistry, Analytical
Chao Wang, Yu Liang, Baofeng Zhao, Zhen Liang, Lihua Zhang, Yukui Zhang
Summary: This study successfully prepared ethane-bridged hybrid monoliths with well-defined large mesopores, which achieved high peak capacity and separation efficiency in top-down proteomic analysis.
ANALYTICAL CHEMISTRY
(2022)
Article
Cell Biology
Mengqi Luo, Tao Su, Shisheng Wang, Jianhai Chen, Tianhai Lin, Qingyuan Cheng, Younan Chen, Meng Gong, Hao Yang, Fuping Li, Yong Zhang
Summary: This study explores the proteomics of human spermatozoa exposed to various stressors and investigates different protein extraction methods. The results suggest that the use of urea buffer combined with ultrasonication provides the most comprehensive coverage of the sperm proteome. Furthermore, the study finds that antibiotics may have an impact on sperm quality.
Article
Biochemical Research Methods
Joana Pissarra, Juli Pagniez, Elodie Petitdidier, Martial Seveno, Oana Vigy, Rachel Bras-Goncalves, Jean-Loup Lemesre, Philippe Holzmuller
Summary: Leishmaniasis is a significant parasitic disease that threatens the lives of 1 billion people every year. In this study, the total protein content of the secretomes produced by seven different Leishmania species was analyzed, revealing the common core secretome and highlighting the importance of secreted proteins in the pathogenesis of the disease.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Jose Renato S. Passos, Denise D. Guerreiro, Kamila S. Otavio, Pedro C. dos Santos-Neto, Marcela Souza-Neves, Federico Cuadro, Richard Nunez-Olivera, Martina Crispo, Maria Julia B. Bezerra, Renato F. Silva, Laritza F. Lima, Jose Ricardo Figueiredo, Ivan C. Bustamante-Filho, Alejo Menchaca, Arlindo A. Moura
Summary: The present study characterized the major proteome of preimplantation ovine embryos grown in vitro. The analysis identified proteins that are involved in translation, protein transport, and stabilization. Functional cluster analysis revealed differences between the in vitro and in vivo proteomes of sheep embryos, indicating that in vitro derived blastocysts have distinct protein profiles compared to those produced in vivo.
REPRODUCTION IN DOMESTIC ANIMALS
(2022)
Article
Biochemical Research Methods
Jing Gao, Yuqiu Wang, Shu Zhang, Zhicheng Yang, Hongwen Zhu, Xuanyang Luo, Dayun Lu, Qiang Gao, Hu Zhou
Summary: A one-dimensional online alkaline-pH reverse phase nanoelectrospray-tandem mass spectrometry (alkaline-pH-MS/MS) method was developed for global proteomic analysis. Compared to conventional low-pH-MS/MS, alkaline-pH-MS/MS showed better enrichment for amino acid variants and protein groups.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemical Research Methods
Jianyu Fang, Songlin Qiao, Keling Wang, Rui Li, Lei Wang, Haili Li, Gaiping Zhang
Summary: This study identified 3731 lysine acetylation sites on 1421 cellular proteins in PRRSV-infected PAMs, indicating the involvement of these acetylated proteins in host immune responses and energy metabolism. These findings will help in understanding PRRSV pathogenesis and identifying new cellular targets for anti-PRRSV therapeutics.
Article
Immunology
Pengfei Li, Zhifang Hao, Jingyu Wu, Chen Ma, Yintai Xu, Jun Li, Rongxia Lan, Bojing Zhu, Pengyu Ren, Daidi Fan, Shisheng Sun
Summary: This study systematically investigated the protein expression profiles of M1 and M2 phenotypes in human THP-1 and mouse RAW264.7 macrophages, revealing significant divergences between the two cell lines while also identifying some common up-regulated proteins in M1 macrophages. These data serve as important resources for further studies of macrophage-associated diseases in experimental pathology using human and mouse cell line models.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Engineering, Biomedical
Hebatullah Hussein, Anil Kishen
Summary: This study found that engineered bioactive chitosan-based nanoparticles can modulate the protein and cytokine profiles of macrophages, reducing inflammation caused by apical periodontitis and promoting the migration of periodontal ligament fibroblasts.
BIOACTIVE MATERIALS
(2022)
Article
Agriculture, Dairy & Animal Science
Andrzej Gawor, Anna Ruszczynska, Anna Konopka, Grzegorz Wryk, Marian Czauderna, Ewa Bulska
Summary: This study investigated the effects of different dietary supplements, including selenium, on protein expression in animal tissues using global differential proteomic experiments and protein-protein interaction network analysis. Selenium was found to play a significant role in regulating important biological functions such as cell adhesion and heart development.
Article
Food Science & Technology
Priscila R. dos Santos-Donado, Carlos M. Donado-Pestana, Rebeca Kawahara, Livia Rosa-Fernandes, Giuseppe Palmisano, Flavio Finardi-Filho
Summary: Biofortification has led to the development of quality protein maize (QPM) with essential amino acids. Study comparing QPM cultivars with a non-QPM hybrid in Brazil showed compositional equivalence with differential expression of proteins, indicating superior nutritional value and susceptibility to pathogens.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)