Article
Chemistry, Multidisciplinary
Jie Li, Weize Yuan, Shao-Xiong Lennon Luo, Mate J. Bezdek, Alexander Peraire-Bueno, Timothy M. Swager
Summary: We report a wireless lateral flow device that converts enzyme-catalyzed reactions into changes in radio frequency identification (RFID) circuit resonance for detecting biological signals. This method enables rapid, portable, and quantitative detection of biological targets.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Chemistry, Multidisciplinary
Qiu-Yi Duan, Ya-Xuan Zhu, Bai-Hui Shan, Yuxin Guo, Ke-Fei Xu, Hao-Ran Jia, Fu-Gen Wu
Summary: Researchers have developed a metabolism nanoregulator to reallocate cell respiration substrates for cancer therapy. By inhibiting mitochondrial oxidative phosphorylation and regulating mitochondrial function, this nanoregulator reduces oxygen consumption and glucose metabolism, effectively killing cancer cells.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Jiangjiang Zhang, Zhentao Huang, Yangzhouyun Xie, Xingyu Jiang
Summary: Ligand modification is a powerful strategy to enhance the catalytic activity of nanozymes, with amine-rich ligands favoring higher activity of gold nanoparticles. Critical guidelines for surface modification of nanozymes were established in this study.
Article
Plant Sciences
Andrej Pavlovic, Ondrej Kocab
Summary: The pitcher traps of Nepenthes species contain a high amount of AOX enzyme and a low amount of COX II enzyme, but have lower abundance of photosynthesis-related proteins. This finding reveals the important role of AOX in the traps.
Article
Engineering, Environmental
Zhuoying Wu, Duc Nguyen, Shilva Shrestha, Lutgarde Raskin, Samir Kumar Khanal, Po-Heng Lee
Summary: We introduced a concept called "nanaerobic digestion", which refers to the enhanced methane production in animal rumen and engineered digesters that is associated with the co-occurrence of methanogens and nanaerobes in a unique ecological niche with very low levels of dissolved oxygen. We analyzed meta-omic datasets and found that animal rumen shares similarities with oxygenated digesters, suggesting that trace levels of oxygen play a role in efficient digestion in ruminants. Prevotellaceae, a bacterial family abundant in rumen, contains many nanaerobes that contribute to efficient methane generation by performing nanaerobic respiration using cytochrome bd oxidase.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Xiaotong Zhao, Dawei Ma, Guoyuan Ju, Bo Gui, Zhangyu Yao, Jia Zhang, Yanbin Zhao, Fangzhou Liu, Xiao Zhang
Summary: This study reveals a new mechanism of cisplatin resistance related to energy metabolism, showing enhanced synthesis of key enzymes, particularly cytochrome oxidase (CCO), in drug-resistant cancer cells. Inhibiting CCO activity can reverse drug resistance in cancer cells.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2023)
Article
Plant Sciences
Alejandro Torrado, Macarena Iniesta-Pallares, Adrian Velazquez-Campoy, Consolacion Alvarez, Vicente Mariscal, Fernando P. Molina-Heredia
Summary: This study investigates the function and classification of cytochrome c(6)-like proteins in photosynthetic cyanobacteria, and provides new data on the phylogeny, genome localization, and functional properties of these proteins. The findings suggest that cytochrome c(6)-like proteins may interact with the cytochrome b(6)f complex in a similar way as cytochrome c(6), and cytochrome c(6)D can react with the specific copper-oxidase in heterocysts.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Biochemical Research Methods
Xiaohuan Huang, Ying Han, Junrong Li, Mingliang Tang, Guangyan Qing
Summary: Increasing evidence supports the critical role of saccharides in tumor progression, and their identification and recognition have paved the way for targeted drug preparations. Achieving fluorescence sensing of saccharides has been the focus of extensive research, leading to the design of molecular probes and nanoparticles made of different materials. This paper discusses the current status of carbohydrate sensing based on fluorescence sensing mechanisms, including phenylboronic acid-based and enzyme-based sensing platforms, and highlights efforts to improve recognition mechanisms and sensing properties.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Ankita J. Sachla, Yuanchan Luo, John D. Helmann
Summary: Cell physiology heavily relies on metalloenzymes, which can be disrupted by imbalances in metal ion pools. Bacillus subtilis requires manganese for growth and has mechanisms for import and efflux to maintain homeostasis. Dysfunctional cytochrome aa(3)-type quinol oxidase plays a central role in metal-induced intoxication.
MOLECULAR MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Henglong Xiang, Sijun Huang, Dongwei Zhu, Liqiang Yu, Rui Liu, Yi Guo, Li Xu
Summary: This study confirmed that gold nanoparticles (AuNPs) can accelerate the reaction between [Ag(NH3)(2)](+) and glucose under alkaline conditions, known as the Tollens' reaction, and proposed a possible mechanism. [Ag(NH3)(2)](+) acts as an electron acceptor during glucose oxidation catalyzed by AuNPs, instead of O-2, accompanied by hydrogen transfer. The synthesized silver nanoparticles (AgNPs) can also catalyze this process through a unique cascading catalysis mechanism in the Tollens' reaction. A simple and heatless glucose colorimetric assay is established based on the plasmonic band of AgNPs, with a linear range of 0.6-22.2 μM and a limit of detection of 0.32 μM.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Andrej Pavlovic, Jana Jaksova, Martin Hrivnacky, Lubomir Adamec
Summary: Many carnivorous plants, including Utricularia reflexa, have a high abundance of alternative oxidase (AOX) in their traps, which are metabolically active and have high respiratory rates. This is in contrast to normal plants, which primarily use the cytochrome c oxidative pathway. The exact role of AOX in carnivorous plant traps remains to be investigated.
PLANT SIGNALING & BEHAVIOR
(2022)
Article
Biochemistry & Molecular Biology
Raul Covian, Lanelle O. Edwards, Bertrand M. Lucotte, Robert S. Balaban
Summary: The catalytic cycle of cytochrome c oxidase (COX) involves multiple intermediate states of the heme a3-CuB binuclear center. The fully reduced species of hemes a or a3, Peroxy (P), and Ferryl (F) intermediates are observed in respiration, while the R form is only detectable under nearly anoxic conditions. The reactions involving P and F species are dependent on transmembrane potential and slower than the oxidation of the R intermediate.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2023)
Article
Chemistry, Multidisciplinary
Jesus Cases Diaz, Monica Gimenez-Marques
Summary: Alternative synthetic procedures enable the formation of protein@HKUST-1 biocomposites, overcoming the challenge of mineralization in water for hydrolytically unstable MOFs.
CHEMICAL COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Sibiao Yue, Lei Wang, George N. DeMartino, FangZhou Zhao, Yi Liu, Matthew H. Sieber
Summary: The regulation of cellular proteostasis is connected to the metabolic state of quiescent cells in vivo. The authors demonstrate that shifts in the ubiquitin-proteome system are linked to mitochondrial metabolic changes and respiratory quiescence. Cellular proteostasis defects and mitochondrial dysfunction play a role in infertility, cancer, and age-related diseases. Through a multi-organism approach, it is shown that dynamic shifts in the ubiquitin proteasome system drive mitochondrial remodeling during cellular quiescence.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Amaravadhi Harikishore, Sherilyn Shi Min Chong, Priya Ragunathan, Roderick W. Bates, Gerhard Gruber
Summary: Mycobacteria demonstrate a high level of adaptation to survive and persist in harsh conditions or under drug exposure. They can adjust their metabolic activity, enzyme composition of the electron transport chain, and levels of survival factors to evade the effects of drug treatments. This study targeted specific residues in the mycobacteria-specific oxidase to inhibit ATP synthesis, showing potential for new drug development against drug-resistant mycobacterial strains.
MOLECULAR DIVERSITY
(2021)