Article
Chemistry, Analytical
Adela Cortes Gimenez-Coral, Pablo Rodriguez-Gonzalez, Adriana Gonzalez Gago, Eva Cernuda Morollon, Elena Lopez-Cancio, Belen Prieto Garcia, J. Ignacio Garcia Alonso
Summary: This study evaluates the use of one- and two-dimensional liquid chromatography for quantifying three stroke outcome predictors in plasma. The isotopically labelled analogues of l-arginine, asymmetric dimethylarginine, and symmetric dimethylarginine are utilized for the quantification. The results show that one-dimensional liquid chromatography, combined with a derivatization step, provides satisfactory results for quantification.
Article
Multidisciplinary Sciences
Ayako Okada, Takatoshi Murata, Khairul Matin, Meu Ariyoshi, Ryoko Otsuka, Mamiko Yamashita, Masayuki Suzuki, Rumi Wakiyama, Ken Tateno, Megumi Suzuki, Hitomi Aoyagi, Hiromi Uematsu, Akiko Imamura, Miki Kosaka, Tomoko Mizukaki, Tsutomu Sato, Hiroshi Kawahara, Nobuhiro Hanada
Summary: This randomized controlled clinical trial aimed to investigate the effect of advanced self-care on atherosclerotic cardiovascular disease-related vascular function markers in patients with early-stage periodontal disease. Results showed that a 3-month periodontal care did not provide better endothelial function in patients with early-stage periodontal disease, despite improvements in periodontal status.
Article
Multidisciplinary Sciences
Patrycja Molek, Pawel Zmudzki, Aleksandra Wlodarczyk, Jadwiga Nessler, Jaroslaw Zalewski
Summary: In patients with ST-segment elevation myocardial infarction, arginine metabolism shifted from nitric oxide synthase towards arginase during ischemia. Lower arginine concentrations at 6-month follow-up were associated with worse long-term outcomes.
SCIENTIFIC REPORTS
(2021)
Article
Pharmacology & Pharmacy
Priya Singh, Soniya Charles, Thirumurthy Madhavan, Ganesh Munusamy-Ramanujam, N. T. Saraswathi, Mariadhas Valan Arasu, Naif Abdullah Al-Dhabi, Aziz Arshad, Jesu Arockiaraj, Kanchana Mala
Summary: The study investigated the impact of TAM and ADOX on PAM in ER-positive breast cancer cells, finding that the two compounds independently trigger cell cycle arrest and downregulate PAM, with a synergistic effect on anti-cancer activity. However, the reduction in ADMA was not directly proportional to the downregulation of PRMT1 mRNA, suggesting a complex interplay with other methyltransferases. Additionally, silk degrees analysis showed that ADOX had a stronger binding affinity with PEMT or BHMT compared to TAM, indicating a potential mechanism for the protective function in breast cancer cells.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Oncology
Yi-Ling Chen, AKaychia T. Lowery, Samuel Lin, Ameae M. Walker, Kuan-Hui E. Chen
Summary: This study found that elevated levels of ADMA impair the proliferation and phagocytosis of macrophages, and can even lead to their death. ADMA also affects the phenotype of macrophages. However, the effects of ADMA on normal epithelial cells and breast cancer cells are relatively minor. Co-culturing tumor cells with primed splenocytes results in increased secretion of ADMA by tumor cells and alterations in arginine metabolism-related gene expression. These findings suggest that tumor cells manipulate macrophage polarization through ADMA secretion to promote tumor tolerance.
CANCER CELL INTERNATIONAL
(2022)
Article
Medicine, Research & Experimental
Yichen Zhao, Xiaoye Ma, Yuchen Zhou, Junchao Xie, Xueyuan Liu, Yanxin Zhao
Summary: Dimethylarginine dimethylamino hydrolase-1 (DDAH-1) plays a protective role in ischemic stroke by regulating ADMA levels and preventing tight junction protein degradation, thus protecting the blood-brain barrier. Supplementation of L-arginine helps restore DDAH-1 function.
LABORATORY INVESTIGATION
(2021)
Article
Biochemistry & Molecular Biology
Juliane Hannemann, Debra J. Skene, Benita Middleton, Edzard Schwedhelm, Anika Laing, Rainer Boeger
Summary: Asymmetric dimethylarginine (ADMA) and symmetric dimethylarginine (SDMA) interfere with nitric oxide (NO) formation from L-arginine via different mechanisms. The plasma concentrations of L-arginine-related amino acid metabolites show diurnal variation, with L-arginine and homoarginine concentrations having a 12-hour period. ADMA and SDMA show a 24-hour rhythmicity. Night shift work does not significantly affect the central suprachiasmatic nuclei clock timing, melatonin and cortisol levels, or blood pressure patterns.
Article
Nutrition & Dietetics
Tian Tian, Jinming Liu, Xinchen Lu, Xinghua Qiu, Jing Wei, Chengrong Wang, Mengyuan Liu, Shengju Yin, Lei Jin, Linlin Wang, Zhiwen Li, Tong Zhu, Aiguo Ren
Summary: This study explores the association between selected essential trace elements (ETEs) and metabolic pathway markers in maternal serum and the likelihood of Neural Tube Defects (NTDs). The results suggest that higher concentrations of selenium (Se) are associated with a decreased chance of NTDs, while certain metabolic pathway markers are associated with an increased risk of NTDs. The study also suggests that the arginine pathway may mediate the association between Se and NTD occurrence.
CLINICAL NUTRITION
(2022)
Article
Chemistry, Medicinal
Assaf Buch, Tali Ganz, Julio Wainstein, Suzan Gilad, Rona Limor, Gabi Shefer, Mona Boaz, Naftali Stern
Summary: Low serum l-arginine and high asymmetric dimethylarginine can predict microvascular complications in type 2 diabetes mellitus. This study investigated the effects of diet and physical activity on overweight/obese and T2DM subjects. The results showed that alcohol lowers l-arginine levels, while protein intake increases l-arginine and asymmetric dimethylarginine levels. Lifestyle modification only reduces asymmetric dimethylarginine levels in subjects who achieve weight loss >5%.
JOURNAL OF MEDICINAL FOOD
(2022)
Article
Environmental Sciences
Servet Birgin Iritas, Aybike Dip, Meside Gunduzoz, Lutfiye Tutkun, Vugar Ali Turksoy, Serdar Deniz, Gulsum Tekin, Ozgur Oztan, Ali Unlu
Summary: The study revealed that long-term exposure to TCE might be an important risk factor for cardiovascular diseases by increasing methylated arginine levels.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL HEALTH RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Maciej Bladowski, Ewa Szahidewicz-Krupska, Jerzy Wisniewski, Paulina Fortuna, Justyna Chojdak-Lukasiewicz, Slawomir Budrewicz, Mariusz Fleszar, Adrian Doroszko
Summary: This study aimed to analyze the differences in the expression of the nitric oxide metabolic pathway in platelets and plasma between ischemic stroke patients and non-stroke individuals. It also investigated the relationship between these parameters and platelet aggregation. The results showed that stroke patients had higher levels of NOS inhibitors in platelets, and stroke management increased the bioavailability of nitric oxide in platelets.
Article
Medicine, General & Internal
Juliane Hannemann, Elin H. Thorarinnsdottir, Andre F. S. Amaral, Edzard Schwedhelm, Lena Schmidt-Hutten, Heike Stang, Bryndis Benediktsdottir, Ingibjoerg Gunnarsdottir, Thorarinn Gislason, Rainer Boeger
Summary: This study found that plasma concentrations of ADMA and SDMA were elevated in patients with the overlap of COPD and OSA, which may lead to reduced production of nitric oxide, increased pulmonary vasoconstriction, and disease progression.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Peripheral Vascular Disease
Vasthi Lopez, Elena Uribe, Fernando A. Moraga
Summary: This study aimed to investigate the mechanisms of high altitude-induced hypertension, revealing that hypoxia-induced hypertensive rats had lower nitric oxide levels and higher concentrations of homocysteine and ADMA in their plasma, potentially due to the altered activity of arginase II.
HYPERTENSION RESEARCH
(2021)
Article
Multidisciplinary Sciences
Miyuki Yokoro, Naoto Otaki, Megumu Yano, Tomomi Imamura, Norikazu Tanino, Keisuke Fukuo
Summary: Plasma ADMA levels are significantly associated with sarcopenia in community-dwelling older women.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Forough Jahandideh, Sareh Panahi, Ronan M. N. Noble, Ferrante S. Gragasin, Rachel G. Khadaroo, Kimberly F. Macala, Stephane L. Bourque
Summary: Sepsis is associated with circulatory dysfunction and organ injury, and the loss of vasoregulatory mechanisms contributes to decreased organ perfusion. This study investigated the development of vascular dysfunction as sepsis progressed to septic shock and found that the mesenteric vasculature exhibited dysregulation before the renal and carotid arteries.
Article
Pathology
Emma J. de Ruiter, Frans J. Mulder, Bregje M. Koomen, Ernst-Jan Speel, Mari F. C. M. van den Hout, Reinout H. de Roest, Elisabeth Bloemena, Lot A. Devriese, Stefan M. Willems
Summary: This study compared the performance of two PD-L1 standardized assays and one laboratory-developed test in head and neck squamous cell carcinoma, revealing moderate concordance between different staining assays and considerable differences in PD-L1 positivity when using clinically relevant cutoffs. These findings suggest caution is needed when using PD-L1 expression to guide clinical practice.
Article
Otorhinolaryngology
Rik J. L. van der Lans, Michelle S. D. Engel, Johannes A. Rijken, Erik F. Hensen, Elisabeth Bloemena, Marein van der Torn, Charles R. Leemans, Conrad F. G. M. Smit
Summary: Middle ear adenomatous neuroendocrine tumors (MEANTs) are rare temporal bone tumors with uncertain clinical behavior and prognosis. Complete surgical resection is the preferred treatment, but recurrence rates are high, and there are currently no effective prognostic biomarkers.
HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
(2021)
Article
Oncology
Irene H. Nauta, Danielle A. M. Heideman, Arjen Brink, Berdine van der Steen, Elisabeth Bloemena, Senada Koljenovic, Robert J. Baatenburg de Jong, C. Rene Leemans, Ruud H. Brakenhoff
Summary: The prevalence of HPV in oral cavity squamous cell carcinoma is low, and neither HPV status nor p16 status affects outcome, making it seem irrelevant for clinical management to determine HPV status in OCSCC.
INTERNATIONAL JOURNAL OF CANCER
(2021)
Article
Medicine, General & Internal
Floris F. van den Brand, Romee J. A. L. M. Snijders, Ynto S. de Boer, Bart J. Verwer, Carin M. J. van Nieuwkerk, Elisabeth Bloemena, Sjoerd D. Kuiken, Joost P. H. Drenth, Gerd Bouma
Summary: In patients with autoimmune hepatitis (AIH) who achieve biochemical remission for at least 2 years, histological normalization may serve as a predictor for successful treatment withdrawal. Two-thirds of patients with histological normalization remained in remission during the follow-up period, while those with persistent histological inflammatory activity were unable to withdraw medication. More research is needed to identify predictors of relapse in these patients.
EUROPEAN JOURNAL OF INTERNAL MEDICINE
(2021)
Article
Oncology
Rutger Mahieu, Inne J. den Toom, Koos Boeve, Daphne Lobeek, Elisabeth Bloemena, Maarten L. Donswijk, Bart de Keizer, W. Martin C. Klop, C. Rene Leemans, Stefan M. Willems, Robert P. Takes, Max J. H. Witjes, Remco de Bree
Summary: Comparative study on the management strategies for early-stage oral squamous cell carcinoma patients showed that sentinel lymph node biopsy (SLNB) is more effective in controlling the contralateral neck than elective neck dissection (END). The incidence and prognosis of CRR differ between patients detected by SLNB or bilateral END, highlighting the importance of proper staging of the contralateral clinically negative neck.
FRONTIERS IN ONCOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
Hannah Dekker, Engelbert A. J. M. Schulten, Christiaan M. ten Bruggenkate, Elisabeth Bloemena, Leo J. van Ruijven, Nathalie Bravenboer
Summary: This study assessed mineralization and trabecular microarchitecture in atrophic edentulous mandibles using microcomputed tomography. Results showed lower bone volume fraction and trabecular number in molar regions, and higher trabecular separation, degree of anisotropy, and cortical BMD. Mandibular height was negatively correlated with trabecular number and connectivity density in premolar regions.
ARCHIVES OF ORAL BIOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Ilkay Evren, Elisabeth R. Brouns, Jos B. Poell, Leon J. Wils, Ruud H. Brakenhoff, Elisabeth Bloemena, Jan G. A. M. de Visscher
Summary: This study aimed to investigate the associations between patients' demographics and habits and the clinical aspect and histopathological characteristics of oral leukoplakia (OL) at patients' first visit. The results showed that different types of OL were associated with smoking, location, and size. However, clinical characteristics cannot substitute microscopic examination of biopsies.
Article
Oncology
Stijn van Weert, Matthijs Valstar, Birgit Lissenberg-Witte, Elisabeth Bloemena, Laura Smit, Jacqueline van der Wal, Marije Vergeer, Ludi Smeele, C. Rene Leemans
Summary: The aim of this study was to analyze prognosticators in acinic cell carcinoma (AciCC) in two head and neck referral centers in Amsterdam. The majority of AciCC cases were T1-T2, and the most affected organ was the parotid gland. The study found that AciCC of the head and neck, excluding high-grade transformation, had an excellent prognosis and acceptable long-term results.
Article
Oncology
Rosanne C. Schoonbeek, Dominique V. C. de Jel, Boukje A. C. van Dijk, Stefan M. Willems, Elisabeth Bloemena, Frank J. P. Hoebers, Esther van Meerten, Berit M. Verbist, Ludi E. Smeele, Gyorgy B. Halmos, Matthias A. W. Merkx, Sabine Siesling, Remco De Bree, Robert P. Takes
Summary: The incidence of head and neck cancer during the first COVID-19 wave in the Netherlands was significantly lower than expected, and the expected increase in incidence during the remainder of 2020 was not observed. Despite the overloaded healthcare system, standard treatment for head and neck cancer patients could be delivered within a shorter time interval.
RADIOTHERAPY AND ONCOLOGY
(2022)
Article
Oncology
Rogier Butter, Liesbeth M. Hondelink, Lisette van Elswijk, Johannes L. G. Blaauwgeers, Elisabeth Bloemena, Rieneke Britstra, Nicole Bulkmans, Anna Lena van Gulik, Kim Monkhorst, Mathilda J. de Rooij, Ivana Slavujevic-Letic, Vincent T. H. B. M. Smit, Ernst-Jan M. Speel, Erik Thunnissen, Jan H. von der Thusen, Wim Timens, Marc J. van de Vijver, David C. Y. Yick, Aeilko H. Zwinderman, Danielle Cohen, Nils A. 't Hart, Teodora Radonic
Summary: This study demonstrates the impact of a pathologist's personality on the interobserver variability and diagnostic accuracy of immunostaining in the context of PD-L1 in NSCLC.
Article
Oncology
Jos B. Poell, Leon J. Wils, Arjen Brink, Ralf Dietrich, Christine Krieg, Eunike Velleuer, Ilkay Evren, Elisabeth R. Brouns, Jan G. de Visscher, Elisabeth Bloemena, Bauke Ylstra, Ruud H. Brakenhoff
Summary: This study developed a noninvasive genetic assay to detect genetically altered fields in the oral cavity. The assay demonstrated high accuracy and predicted the development of oral squamous cell carcinoma in high-risk individuals. This method can be used for cancer screening in high-risk populations and to map the extent of lesions beyond what is visible.
INTERNATIONAL JOURNAL OF CANCER
(2023)
Article
Oncology
Leon J. Wils, Jos B. Poell, Arjen Brink, Ilkay Evren, Elisabeth R. Brouns, Jan G. A. M. de Visscher, Elisabeth Bloemena, Ruud H. Brakenhoff
Summary: Oral leukoplakia is a common potentially malignant oral disorder, with a 1% to 5% annual malignant transformation rate. Although no definitive risk stratification model exists, next-generation sequencing can be used to predict the risk for malignant transformation. Based on genetic alterations and dysplasia, we developed a prediction model for malignant transformation in oral leukoplakia.
CLINICAL CANCER RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Bina Kashyap, Konsta Naumanen, Jopi Mikkonen, Hannah Dekker, Engelbert Schulten, Elisabeth Bloemena, Sanna Pasonen-Seppanen, Arja Kullaa
Summary: This study aimed to investigate the immunohistochemical expression of MUC1, CD44, and HA in irradiated oral mucosa and their role in long-term effects after radiotherapy. The results showed significantly elevated staining intensities of MUC1, CD44, and HA in irradiated tissue compared to controls.
Article
Dentistry, Oral Surgery & Medicine
Leon J. Wils, Jos B. Poell, Laura A. N. Peferoen, Ilkay Evren, Elisabeth R. Brouns, Jan G. A. M. de Visscher, Erik H. van Der Meij, Ruud H. Brakenhoff, Elisabeth Bloemena
Summary: Oral leukoplakia is a common potentially malignant disorder, and classic epithelial dysplasia and differentiated dysplasia are important predictors of its malignant transformation.
JOURNAL OF ORAL PATHOLOGY & MEDICINE
(2023)
Article
Dentistry, Oral Surgery & Medicine
Ilkay Evren, Ahmad M. Najim, Jos B. Poell, Elisabeth R. Brouns, Leon J. Wils, Laura A. N. Peferoen, Ruud H. Brakenhoff, Elisabeth Bloemena, Erik H. van Der Meij, Jan G. A. M. de Visscher
Summary: This study evaluated whether regular follow-up of oral leukoplakia resulted in early detection of malignant transformation. The results showed that patients followed up by their dentist had smaller leukoplakia size, more hyperkeratosis, and less moderate/severe dysplasia. Malignant transformation occurred most frequently in patients followed up at the hospital, least frequently in patients followed up by their dentist, and intermediate in patients lost to follow-up. Regular follow-up allowed for early detection of malignant transformation, and follow-up by the dentist was found to be feasible.