Isolation and characterization of twenty novel microsatellite markers for half-smooth tongue sole (Cynoglossus semilaevis)

Twenty novel microsatellite markers for half-smooth tongue sole (Cynoglossus semilaevis) were isolated and characterized from a simple sequence repeat-enriched library constructed with (GA)(15) and (CA)(15). The polymorphism was assessed using 32 individuals collected from one locality along the Chinese coast. The result showed the number of alleles ranged from 2 to 14, with an average of 8.7 alleles/locus. The values of observed and expected heterozygosities ranged from 0.2609 to 1.0000 and from 0.2367 to 0.9716, respectively. Six microsatellite loci departed significantly from Hardy-Weinberg equilibrium. No linkage disequilibrium was found. These markers will be useful for the conservation studies and population structure assessment for this species.