期刊
CONSERVATION GENETICS
卷 10, 期 3, 页码 577-580出版社
SPRINGER
DOI: 10.1007/s10592-008-9573-6
关键词
Caretta caretta; Loggerhead turtle; Microsatellites; Cross-amplification
资金
- Georgia Department of Natural Resources
- Daniel B. Warnell School of Forestry and Natural Resources
- Florida Fish and Wildlife Conservation Commission [135]
- UGA Institutional Animal Care and Use Committee [A2006-10002-0]
The loggerhead sea turtle (Caretta caretta) is a federally threatened species and listed as endangered by the World Conservation Union (IUCN). We describe primers and polymerase chain reaction (PCR) conditions to amplify 11 novel tetranucleotide microsatellite loci from the loggerhead sea turtle. We tested primers using samples from 22 females that nested at Melbourne Beach, Florida (USA). Primer pairs yielded an average of 11.2 alleles per locus (range of 4-24), an average observed heterozygosity of 0.83 (range 0.59-0.96), and an average polymorphic information content of 0.80 (range 0.62-0.94). We also demonstrate the utility of these primers, in addition to primers for 15 loci previously described, for amplifying microsatellite loci in four additional species representing the two extant marine turtle families: olive ridley (Lepidochelys olivacea), hawksbill (Eretmochelys imbricata), green turtle (Chelonia mydas), and leatherback (Dermochelys coriacea).
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