Article
Fisheries
Chihoon Lee, Young-Don Lee
Summary: By using scanning electron microscopy, the morphological characteristics of micropyle and micropylar cone during fertilization were examined in two species of groupers, Epinephelus akaara and E. septemfasciatus. The results showed that these two species have similar micropylar size and structure, and the micropylar cone plays a role in preventing polyspermy.
AQUACULTURE RESEARCH
(2022)
Article
Virology
Si-Woo Kim, Soo-Jin Kim, Myung-Joo Oh
Summary: Immunization at low temperature with NNV in sevenband grouper resulted in increased resistance against NNV infection, leading to lower mortality rates and decreased disease symptoms.
Article
Fisheries
Xinxin Shan, Tengfei Xu, Zhiyuan Ma, Xinhui Zhang, Zhiqiang Ruan, Jieming Chen, Qiong Shi, Xinxin You
Summary: This study aimed to improve the ammonia tolerance of groupers through genomic selection, predicting the genomic estimated breeding values using genome-wide loci and single nucleotide polymorphisms. Results showed that the method based on GWAS-informative SNPs performed better in cost-efficiency for genomic selection.
Article
Fisheries
Weiwei Zhang, Shuyuan Fu, Xin Fan, Jie Huang, Yesong Liang, Xin Wen, Jian Luo
Summary: Through cold shock, it is possible to efficiently produce sterile triploid hybrids, and analysis shows that the abnormal development of these sterile fish's gonadal tissues may be due to disorders in gamete generation during meiosis.
Article
Biochemistry & Molecular Biology
Yue-Xuan Wang, Sui-Feng Xu, Ye-Wen Wang, Yun-Xiang Jiang, Qi-Wei Qin, Shi-Na Wei
Summary: This study aimed to investigate the protective effect of curcumin against Singapore grouper iridovirus-induced intestinal damage in orange-spotted groupers. The results showed that curcumin significantly alleviated intestine damage, repaired intestinal barrier dysfunction, suppressed intestinal cells apoptosis and inflammatory response, protected intestinal cells from oxidative injury, and restored the disruption of the intestinal microbiota caused by the virus. These findings provide a theoretical basis for the use of curcumin in aquaculture to prevent SGIV-induced intestinal injury.
Article
Fisheries
Pengfei Duan, Yongsheng Tian, Zhentong Li, Shuai Chen, Linlin Li, Xinyi Wang, Linna Wang, Yang Liu, Jieming Zhai, Wensheng Li, Qingbin Wang, Wenhui Ma, Zunfang Pang
Summary: This study conducted comparative transcriptome analyses of the liver, muscle, and brain tissues of Jinhu grouper and tiger grouper at different temperatures, revealing the molecular mechanisms and differences in temperature adaptation between the two species. The results suggest that Jinhu grouper may have better temperature tolerance and show higher expression of heat shock protein genes in brain tissue.
Article
Environmental Sciences
Xiangyu Zhu, Xinhai Zhang, Mingru Chen, Xiaomei Xu
Summary: Auditory brainstem response (ABR) technology was used to establish a fish auditory threshold detection system and study the auditory threshold of wild yellow grouper Epinephelus awoara in the Taiwan Strait. ABRs of 10 wild yellow groupers (TL150-200 mm) were recorded and analyzed in response to 12 different frequencies of sound stimulation ranging from 100 to 2000 Hz. The results showed that the auditory threshold of the fish increased with frequency. The fish was most sensitive to low frequency sound signals of 100-300Hz, with a threshold of 90 dB re 1 mu Pa.
FRONTIERS IN MARINE SCIENCE
(2023)
Article
Fisheries
Shuai Chen, Yongsheng Tian, Zhentong Li, Yang Liu, Ziqi Li, Pengfei Duan, Linlin Li, Xinyi Wang, Linna Wang, Xinlei He, Xia Zhao, Wensheng Li, Qingbin Wang
Summary: Hybridization is a widely used and effective method in grouper breeding. In this study, the growth characteristics of Jinhu grouper, Hulong grouper, and brown-marbled grouper were compared under industrial conditions. It was found that Jinhu grouper exhibited a rapid growth rate, high survival rate, and low temperature resistance.
Article
Fisheries
Jianpeng Huang, Eric Amenyogbe, Zhenwei Wen, Guanghai Ou, Yu Li, Xingtao Jiang, Ruitao Xie, Gang Chen
Summary: This study investigated the effect of Bacillus licheniformis supplementation on hybrid grouper's culture water quality, growth performance, and intestinal health, and found that it significantly improved water quality, enhanced growth performance, and positively influenced intestinal health.
AQUACULTURE REPORTS
(2023)
Article
Multidisciplinary Sciences
Xiaobo Yan, Junjiang Yang, Xiaohui Dong, Beiping Tan, Shuang Zhang, Shuyan Chi, Qihui Yang, Hongyu Liu, Yuanzhi Yang
Summary: The study aimed to explore the optimum protein requirement of juvenile grouper. Results showed that as the protein level increased, there were changes in growth, feed utilization, and nutrient metabolism. The optimum protein level for juvenile grouper was found to be 521.84 g/kg.
SCIENTIFIC REPORTS
(2021)
Article
Fisheries
Yaqiu Zhang, Yiqin Deng, Juan Feng, Zhixun Guo, Can Mao, Haoxiang Chen, Ziyang Lin, Jianmei Hu, Youlu Su
Summary: The deletion of cqsA in V. harveyi resulted in enhanced virulence, including increased survival and resistance abilities, and reduced interference with the host immune system.
Article
Biochemistry & Molecular Biology
Yumeng Zhang, Hang Zhou, Yu Liu, Lulu Zhu, Jiongting Fan, Huajing Huang, Wen Jiang, Junming Deng, Beiping Tan
Summary: An 8-week feeding experiment was conducted to investigate the effect of dietary histamine on the growth performance, digestive physiology function and muscle quality of hybrid grouper. The results showed that high levels of histamine significantly decreased the activities of digestive enzymes, damaged the gastric and intestinal mucosa, disrupted the intestinal tight junction structure, and affected the muscle quality of the grouper.
Article
Fisheries
Yuexuan Wang, Suifeng Xu, Yewen Wang, Xuchao Zhang, Jiatao Chen, Qiwei Qin, Shina Wei
Summary: This study found that curcumin, extracted from the traditional Chinese medicine turmeric, has various biological activities, including immunoenhancement, antioxidant, and antibacterial properties. The results showed that curcumin can enhance fish immunity, reduce mortality caused by viral infection, and repair liver injury caused by the virus. This provides a theoretical foundation for the functional development of curcumin in aquaculture.
Article
Biotechnology & Applied Microbiology
Linna Wang, Zhentong Li, Yang Liu, Shuai Chen, Linlin Li, Pengfei Duan, Xinyi Wang, Wensheng Li, Qingbin Wang, Jieming Zhai, Yongsheng Tian
Summary: In this study, the genome assembly of the potato grouper was successfully generated using multiple sequencing and capture technologies. Several genes associated with fast growth and stress resistance were identified. Additionally, a large number of potential antimicrobial peptide genes were discovered. This research provides an important reference for selective breeding and the development of marine drugs.
Article
Fisheries
Honglin Han, Liqun Wang, Suifeng Xu, Shaowen Wang, Min Yang, Chengzong Han, Qiwei Qin, Shina Wei
Summary: In this study, Ec-MARCO was identified and characterized in the orange-spotted grouper, and its important role in regulating the immune response against SGIV infection was demonstrated.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Review
Fisheries
Bo Zhang, Changwei Shao, Songlin Chen, Baolong Bao
Summary: Pleuronectiformes, also known as flatfishes, is an important species in mariculture with unique body asymmetry, making it valuable for scientific research. It also has great potential for industrial aquaculture due to its rapid growth, rich nutrition, delicious taste, and high-quality protein that are favored by consumers worldwide. However, the large-scale breeding and expanding production have led to the emergence of abnormal body color flatfishes, which greatly affect their commercial value. This review discusses the environmental factors influencing body color abnormalities, the possible genetic mechanisms, and proposes genomic screening measures to prevent abnormal body coloration.
REVIEWS IN AQUACULTURE
(2022)
Article
Genetics & Heredity
Shuo Li, Kaiqiang Liu, Aijun Cui, Xiancai Hao, Bin Wang, Hong-Yan Wang, Yan Jiang, Qian Wang, Bo Feng, Yongjiang Xu, Changwei Shao, Xuezhou Liu
Summary: Yellowtail kingfish is a valuable species for open ocean aquaculture. The assembly of its high-precision genome provides important insights for genetic breeding research and large-scale aquaculture.
FRONTIERS IN GENETICS
(2022)
Article
Fisheries
Daiqiang Zhang, Bao Shi, Peng Shao, Changwei Shao, Chongnv Wang, Jing Li, Xuezhou Liu, Xiaodong Ma, Xinyu Zhao
Summary: This study comprehensively analyzed the transcriptomic modulation of the brain-pituitary-gonad (BPG)-liver axis related to ovary maturation in Cynoglossus semilaevis. Through miRNA sequencing, a large number of miRNAs were identified, some of which were found to simultaneously regulate multiple mRNAs and participate in various biological functions. Important mRNAs related to reproduction were screened based on target gene relationships. KEGG pathway analysis revealed signaling pathways related to steroid hormone biosynthesis, oocyte meiosis, and other reproductive processes. RT-qPCR confirmed the expression patterns of differentially expressed miRNAs and validated the reliability of the sequencing data. Furthermore, the binding site between miR-186-x and igf2r was validated for the first time.
Article
Multidisciplinary Sciences
He Zhou, Qian Wang, Zi-Yu Zhou, Xin Li, Yu-Qing Sun, Gu Shan, Xin-Yi Zheng, Qi Chen, Hai-Jin Liu, Wei Wang, Chang-Wei Shao
Summary: Whole genome bisulfite sequencing was used to investigate DNA methylation profiles between haploid and diploid tiger pufferfish, revealing differential methylation related to abnormal development and early embryonic death in haploids. The identified differentially methylated genes were mainly associated with genomic stability maintenance and cell cycle regulation.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Lucas B. Doretto, Arno J. Butzge, Rafael T. Nakajima, Emanuel R. M. Martinez, Beatriz Marques de Souza, Maira da Silva Rodrigues, Ivana F. Rosa, Juliana M. B. Ricci, Aldo Tovo-Neto, Daniel F. Costa, Guilherme Malafaia, Changwei Shao, Rafael H. Nobrega
Summary: In this study, the roles of the Gdnf-Gfr alpha 1 signaling pathway in zebrafish testes were investigated using in vivo, in silico, and ex vivo approaches. The findings showed that Gdnf promotes the creation of new available niches and inhibits late spermatogonial differentiation in zebrafish.
Article
Agriculture, Dairy & Animal Science
Xianghui Zhang, Xiancai Hao, Wenxiu Ma, Tengfei Zhu, Zhihua Zhang, Qian Wang, Kaiqiang Liu, Changwei Shao, Hong-Yan Wang
Summary: This study analyzed the gene expression changes in the kidney of Chinese tongue sole after Vibrio harveyi infection and found rapid up-regulation of immune-related pathways in the early stage, as well as a close connection between the immune system and neuroendocrine system.
Article
Cell Biology
Xiang Liu, Yingyi Huang, Fujian Tan, Hong-Yan Wang, Jian-Yang Chen, Xianghui Zhang, Xiaona Zhao, Kaiqiang Liu, Qian Wang, Shanshan Liu, Francesc Piferrer, Guangyi Fan, Changwei Shao
Summary: This study used single-cell RNA sequencing to analyze the molecular characteristics of ovarian cells in adult Chinese tongue sole, providing insights into the cell-type-specific mechanisms underlying fish oogenesis. The study identified the cellular composition of fish follicle walls and proposed important transcription factors involved in cell identity regulation. The findings also revealed the extensive bidirectional communication between niche cells and germ cells during fish oogenesis and highlighted the coordinated sequential processes across different granulosa cells during fish ovulation. Comparative analysis with macaques showed conserved biological processes in germ cells and granulosa cells across vertebrates.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Lili Tang, Fei Huang, Wuxin You, Ansgar Poetsch, Rafael Henrique Nobrega, Deborah Mary Power, Tengfei Zhu, Kaiqiang Liu, Hong-Yan Wang, Qian Wang, Xiwen Xu, Bo Feng, Manfred Schartl, Changwei Shao
Summary: Research has revealed the involvement of ncRNAs through ceRNA crosstalk in the regulation of sexual development in fish. In the testis of Chinese tongue sole, an up-regulated circular RNA and an lncRNA named AMSDT share the same miRNA response elements with a sex-determining gene, leading to the down-regulation of female sex-related genes and promoting testis differentiation.
Article
Biochemistry & Molecular Biology
Xiaona Zhao, Bo Feng, Qian Wang, Lili Tang, Qian Liu, Wenxiu Ma, Chenghua Li, Changwei Shao
Summary: This study identified the org gene and its associated lncRNA ORG-AS in Chinese tongue sole. The results showed that org is involved in early embryonic development and oogenesis, and is negatively regulated by lncRNA ORG-AS. These findings expand the understanding of the functions of MEGs in bony fish embryonic development and oogenesis, and highlight the importance of lncRNAs in regulating org.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Qian Wang, Qian Liu, Wenxiu Ma, Rui Wang, Shuo Li, Zhongdian Dong, Changwei Shao
Summary: This study identified and analyzed the eef1a1 gene in Chinese tongue sole and investigated its role in gonadal high-temperature perception. The results showed sexually dimorphic expression of eef1a1 with higher expression in the ovary than in the testis. Under high-temperature conditions, eef1a1 was down-regulated in males but not in females. These findings suggest that eef1a1 may have a dual role in external temperature perception and sex differentiation regulation.
Article
Developmental Biology
Qian Qi, Zhongdian Dong, Ning Zhang, Changwei Shao, Wenteng Xu
Summary: In this study, a homologous gene to ptch1, csptch1 x1, was identified in Cynoglossus semilaevis. Expression pattern analysis and in situ hybridization revealed that csptch1 x1 plays an important role in female differentiation and ovarian development, distinct from the function of csptch1 in spermatogenesis.
GENE EXPRESSION PATTERNS
(2022)
Article
Immunology
Tengfei Zhu, Ming Kong, Chen Li, Changwei Shao
Summary: This study investigated the role of exosomal miRNAs in Chinese tongue sole's response to Vibrio harveyi infection. The results demonstrated the important regulatory roles of exosomal miRNAs in the inflammatory and immune responses, and their potential as biomarkers for the prognosis and diagnosis of infection in teleost fish.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Qian Liu, Yue Wang, Leilei Tan, Wenxiu Ma, Xiaona Zhao, Changwei Shao, Qian Wang
Summary: Fish sex determination can be influenced by environmental temperature, and the heat shock proteins (HSPs) play important roles in this process. In this study, we investigated the role of hsc70 and hsc70-like genes in high temperature response and sex determination/differentiation using Chinese tongue sole as model. Our results showed that hsc70 and hsc70-like are key regulators linking high temperature signals with sex differentiation by regulating the expression of sex-related genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Lucie A. Bergeron, Soren Besenbacher, Jiao Zheng, Panyi Li, Mads Frost Bertelsen, Benoit Quintard, Joseph I. Hoffman, Zhipeng Li, Judy St Leger, Changwei Shao, Josefin Stiller, M. Thomas P. Gilbert, Mikkel H. Schierup, Guojie Zhang
Summary: We sequenced and compared high-coverage genomes of 151 parent-offspring trios from 68 species of vertebrates and found that the per-generation mutation rate varies greatly among species, with males having higher rates than females in mammals and birds. We identified generation time, age at maturity, and species-level fecundity as key factors influencing this variation. Species with larger long-term effective population sizes tend to have lower mutation rates, supporting the drift barrier hypothesis. Domesticated animals with shorter generation times display exceptionally high yearly mutation rates, emphasizing the importance of generation time in mutation rate evolution.
Article
Agriculture, Dairy & Animal Science
Leilei Tan, Qian Liu, Yangbin He, Jingjing Zhang, Jilun Hou, Yuqin Ren, Wenxiu Ma, Qian Wang, Changwei Shao
Summary: In this study, a spermatogenic stem cell line was established from tiger puffer fish, which showed stable growth and the ability to differentiate into other types of cells. This research provides an important research model and foundation for sex determination and breeding control in fish.
